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109253 Papanicolaou's solution 1a Harris' hematoxylin solution

109253
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1092530507
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      Plastic bottle 500 ml
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      1092531007
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          Plastic bottle 1 l
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          1092532507
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              Plastic bottle 2.5 l
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              Description
              Catalogue Number109253
              SynonymsHematoxylin solution acc. to Harris, Colour solution acc. to Papanicolaou, Harris' Hematoxylin solution
              DescriptionPapanicolaou’s solution 1a Harris hematoxylin solution
              OverviewPapanicolaou’s solution 1a Harris hematoxylin solution - for cytology, is a ready-to-use solution used for human-medical cell diagnosis and serve the purpose of the cytological investigation of sample material of human origin, for example, gynecological and clinico-cytological specimen materials. Papanicolaou’s technique is the most used staining procedure for cytological specimen is and is intended for the the staining of exfoliative cells in cytological specimens. In the first step, the cell nuclei are stained either progressively or regressively with a hematoxylin solution, (Hematoxylin solution modified acc.to Gill II, (Cat.no. 105175), Papanicolaou’s solution 1a Harris hematoxylin solution, (Cat.no. 109253) or Papanicolaou’s solution 1b Hematoxylin solution S, (Cat.no. 109254)). Nuclei are stained blue to dark violet. In the progressive hematoxylin staining method, staining is carried out to the endpoint, after which the slide is blued in tapwater. With the regressive method the material is over-stained and the excess of staining solution is removed by acid rinsing steps, followed by the bluing step. The structures of nuclei are more differentiated and better visible by the regressive method. The second staining step is cytoplasmic staining by (Papanicolaou’s solution 2b Orange II solution, (Cat. no. 1106887) or Papanicolaou’s solution 2a Orange G solution (OG6), (Cat. no. 106888)), especially for demonstration of mature and keratinized cells. The target structures are stained orange in different intensities. In the third staining step is used the so-called polychrome solution, a mixture of eosin, light green SF and Bismarck brown, Papanicolaou’s solution 3a polychromatic solution EA 31, (Cat.no 109271), Papanicolaou’s solution 3b polychromatic solution EA 50, (Cat.no. 109272), Papanicolaou’s solution 3c polychromatic solution EA 65,(Cat.no. 109270), or Papanicolaou’s solution 3d polychromatic solution EA 65, (Cat.no.109269). The polychrome solution is used for demonstration of differentiation of squamous cells. Both Papanicolaou’s solution 1a Harris hematoxylin solution(Cat.no. 109253) and Papanicolaou’s solution 1b Hematoxylin solution S (Cat.no. 109254) give a blue to dark violet staining result with clinical specimen material.
              A 500 ml bottle of either solution is sufficient for 1500 -2500 stainings. It is registered as IVD and CE certified product and can be used for clinical diagnostics. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
              References
              Product Information
              HS Code3212 90 00
              Quality LevelMQ400
              Applications
              Applicationfor cytology
              Biological Information
              Physicochemical Information
              Boiling point100 °C (1013 hPa)
              Density1.04 g/cm3 (20 °C)
              pH value2.5 - 3.0 (H₂O, 20 °C)
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              Hazard Pictogram(s) Hazard Pictogram(s)
              Hazard Statement(s)H318: Causes serious eye damage.
              EUH210: Safety data sheet available on request.
              Precautionary Statement(s)P280: Wear eye protection/ face protection.
              P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
              Signal WordDanger
              Storage class10 - 13 Other liquids and solids
              WGKWGK 1 slightly hazardous to water
              Disposal28
              Aqueous solutions: Container D.
              Safety Information
              Product Usage Statements
              Storage and Shipping Information
              StorageStore at +15°C to +25°C.
              Packaging Information
              Transport Information
              Supplemental Information
              Specifications
              Suitability for microscopy (Vaginal smear)passes test
              Nucleiblue to dark violet
              Cyanophilic Cytoplasm (basophilic)blue-green
              Eosinophilic Cytoplasm (acidophilic)pink

              Documentation

              Papanicolaou's solution 1a Harris' hematoxylin solution SDS

              Title

              Safety Data Sheet (SDS) 

              Papanicolaou's solution 1a Harris' hematoxylin solution Certificates of Analysis

              Product Number PackagingSpecificationLot Number
              1092530507 Plastic bottlePDF Specification Document
              1092531007 Plastic bottlePDF Specification Document
              1092532507 Plastic bottlePDF Specification Document

              Instructions for Use

              Title
              109253 Papanicolaou's solution 1a Harris hematoxylin solution for cytology

              Related Products & Applications

              Accessories (Primary)

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              Accessories (Secondary)

              Catalog Number Description  
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              105175 Hematoxylin solution modified acc. to Gill II Show Pricing & Availability
              106887 Papanicolaou’s solution 2b Orange II solution Show Pricing & Availability
              106888 Papanicolaou's solution 2a Orange G solution (OG 6) Show Pricing & Availability
              109254 Papanicolaou's solution 1b Hematoxylin solution S Show Pricing & Availability
              109271 Papanicolaou's solution 3a polychromatic solution EA 31 Show Pricing & Availability

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              Categories

              IVD/OEM Materials & Reagents > Microscopy > Cytology > Cytological Staining Reagents