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481409 PTEN, His•Tag® and S•Tag™, Human Recombinant, S. frugiperda

481409
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481409-10UG
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      Plastic ampoule 10 μg
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      Description
      OverviewFull-length, recombinant, human PTEN (GenBank target symbol = PTEN, ACC No. BC_005821) fused at N-terminus to a His•Tag® and S•Tag sequences and expressed in S. frugiperda insect cells. PTEN is a tumor suppressor that is frequently found mutated in human cancers. It serves as a lipid phosphatase and negative regulator of the PI-3K/Akt pathway. Absence of functional PTEN results in constitutive activation of Akt and mTOR kinases.
      Catalogue Number481409
      Brand Family Calbiochem®
      SynonymsPhosphatase and Tensin Homolog Deleted on Chromosome 10
      References
      ReferencesHarrington, L.S., et al. 2005. Trends Biochem. Sci. 30, 35.
      Osaki, M., et al. 2004. Apoptosis 9, 667.
      Lennon, G., et al. 1996. Genomics 33, 151.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enzyme required to liberate 1 nmol phosphate from PIP₃ per min at 37°C.
      FormLiquid
      FormulationIn 150 mM NaCl, 50 mM Tris-HCl, 5 mM DTT, 1 mM EDTA, 50% glycerol, pH 7.5.
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥80% by SDS-PAGE
      Specific Activity≥75 U/mg protein
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      PTEN, His•Tag® and S•Tag™, Human Recombinant, S. frugiperda SDS

      Title

      Safety Data Sheet (SDS) 

      PTEN, His•Tag® and S•Tag™, Human Recombinant, S. frugiperda Certificates of Analysis

      TitleLot Number
      481409

      References

      Reference overview
      Harrington, L.S., et al. 2005. Trends Biochem. Sci. 30, 35.
      Osaki, M., et al. 2004. Apoptosis 9, 667.
      Lennon, G., et al. 1996. Genomics 33, 151.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision12-June-2008 RFH
      SynonymsPhosphatase and Tensin Homolog Deleted on Chromosome 10
      DescriptionFull-length, recombinant, human PTEN (GenBank target symbol = PTEN, ACC No. BC_005821) fused at N-terminus to a His•Tag® and S•Tag sequences and expressed in S. frugiperda insect cells. PTEN is a tumor suppressor that is frequently found mutated in human cancers. It serves as a lipid phosphatase and negative regulator of the PI-3K/Akt pathway. Absence of functional PTEN results in constitutive activation of Akt and mTOR kinases.
      FormLiquid
      FormulationIn 150 mM NaCl, 50 mM Tris-HCl, 5 mM DTT, 1 mM EDTA, 50% glycerol, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Recommended reaction conditions100 mM Tris-HCl, 10 mM DTT, pH 8.0, 25 µg/ml PTEN, 37°C.
      Purity≥80% by SDS-PAGE
      Specific activity≥75 U/mg protein
      Unit definitionOne unit is defined as the amount of enzyme required to liberate 1 nmol phosphate from PIP₃ per min at 37°C.
      Storage ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesHarrington, L.S., et al. 2005. Trends Biochem. Sci. 30, 35.
      Osaki, M., et al. 2004. Apoptosis 9, 667.
      Lennon, G., et al. 1996. Genomics 33, 151.