506075 Novel Inducer of Death-1, NID-1 - Calbiochem

506075
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Overview

Replacement Information

Key Specifications Table

Empirical Formula
C₁₅H₁₄N₂O₃S

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      Glass bottle 25 mg
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      Description
      OverviewA cell-permeable, nitrothiophenylpropenamide compound that induces active, energy-dependent, and rapid non-apoptotic cell death (EC50 = 1.65 µM) in several transformed (DRD, RKO, H1299) and non-transformed (BJeH ) human cells, in a variety of cancer cell lines, and in mouse embryonic fibroblasts. The cell death appears to be non-necrotic, non-oxidative and is independent of DNA damage or cell proliferation. NID-1 induced cell death, although distinct from macroautophagy, does show PI 3-kinase suppression, ATG5-dependence, p62 degradation, and higher LC3 levels. Also, NID-1-induced death is independent of caspases, Bax and Bak and requires lysosomal activity and cytosolic vacuolization. Rescues mutant-huntingtin-induced cell death in a cell-based model of Huntington’s disease. Shown to increase cathepsin L levels, but does not affect cathepsins B, C, and H.
      Catalogue Number506075
      Brand Family Calbiochem®
      Synonyms(E)-N-(2-ethylphenyl)-3-(5-nitrothiophen-2-yl)acrylamide
      References
      ReferencesVarma, H., et al. 2013. Exp. Cell Res.319, 1759.
      Product Information
      FormYellow solid
      Hill FormulaC₁₅H₁₄N₂O₃S
      Chemical formulaC₁₅H₁₄N₂O₃S
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥98% by HPLC
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Novel Inducer of Death-1, NID-1 - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overview
      Varma, H., et al. 2013. Exp. Cell Res.319, 1759.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision31-October-2013 JSW
      Synonyms(E)-N-(2-ethylphenyl)-3-(5-nitrothiophen-2-yl)acrylamide
      DescriptionA cell-permeable, nitrothiophenylpropenamide compound that induces active, energy-dependent, and rapid non-apoptotic cell death (EC50 = 1.65 µM) in several transformed (DRD, RKO, H1299) and non-transformed (BJeH ) human cells, in a variety of cancer cell lines, and in mouse embryonic fibroblasts. The cell death appears to be non-necrotic, non-oxidative and is independent of DNA damage or cell proliferation. NID-1 induced cell death, although distinct from macroautophagy, does show PI 3-kinase suppression, ATG5-dependence, p62 degradation, and higher LC3 levels. Also, NID-1-induced death is independent of caspases, Bax and Bak and requires lysosomal activity and cytosolic vacuolization. Rescues mutant-huntingtin-induced cell death in a cell-based model of Huntington′s disease. Shown to increase cathepsin L levels, but does not affect cathepsins B, C, and H.
      FormYellow solid
      Intert gas (Yes/No) Packaged under inert gas
      Chemical formulaC₁₅H₁₄N₂O₃S
      Structure formulaStructure formula
      Purity≥98% by HPLC
      SolubilityDMSO (50 mg/ml)
      Storage +2°C to +8°C
      Protect from light
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Toxicity Standard Handling
      ReferencesVarma, H., et al. 2013. Exp. Cell Res.319, 1759.