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105226 New fuchsin (C.I. 42520)

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      Glass bottle 100 g
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      Catalogue Number105226
      DescriptionNew fuchsin (C.I. 42520)
      OverviewThe dry dye New fuchsin (C.I. 42520) for microscopy Certistain®, is used for the preparation of a staining solution that can be used for staining of histological tissue sections or other samples of human origin according to Ziehl-Neelsen, Gram staining as well as in combination with Schiff’s reagent. As a CERTISTAIN® dye it is chemically quality-certified according to highest standards and strict specifications. New fuchsin belongs to the group of triaminotriphenylmethane dyes.
      The dye is used in Ziehl-Neelsen and Gram staining as well as for methods with Schiff’s reagent.
      The cell wall of acid-fast bacteria (AFB) has a high proportion of wax and lipids and hence absorbs dyes only very slowly. As a measure to enhance the absorption of the fuchsin dye and thus the formation of the mycolate-fuchsin complex in the cell wall, the carbolfuchsin solution applied to the specimen is normally heated to evaporation point. If the Kinyoun carbolfuchsin solution is used, the heating of the solution is dispensable. Consequently, the release of hazardous phenolic vapors will be avoided.
      Once the AFB have absorbed the fuchsin dye, it is virtually impossible to decolorize them again, even when they are intensively treated with a decolorizing solution such as e. g. hydrochloric acid in ethanol. Accordingly, AFB are termed as acid- and alcohol-fast for staining, and are stained red in the microscopic visualization. Correspondingly, all non-acid-fast microorganisms are counterstained with an appropriate dye.
      Pretreatment of the specimens with Sputofluol® dissolves the bacteria from the surrounding viscid sputum and cell material. Sputofluol® also has a disinfectant effect, with the result that any microorganisms that are present are killed off. It is registered as IVD and certified as CE product. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
      Product Information
      CAS number3248-91-7
      EC number221-831-7
      Hill FormulaC₂₂H₂₄ClN₃
      Molar Mass365.91 g/mol
      HS Code3212 90 00
      Structure formula ImageStructure formula Image
      Quality LevelMQ400
      Applicationfor microscopy Certistain®
      Biological Information
      Physicochemical Information
      pH value3 - 4 (10 g/l, H₂O, 20 °C)
      Bulk density450 kg/m3
      Solubility10 g/l
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Hazard Pictogram(s) Hazard Pictogram(s)
      Hazard Pictogram(s)
      Hazard Pictogram(s)
      Hazard Statement(s)H315: Causes skin irritation.
      H319: Causes serious eye irritation.
      H335: May cause respiratory irritation.
      H350: May cause cancer.
      H410: Very toxic to aquatic life with long lasting effects.
      Precautionary Statement(s)P201: Obtain special instructions before use.
      P202: Do not handle until all safety precautions have been read and understood.
      P273: Avoid release to the environment.
      P302 + P352: IF ON SKIN: Wash with plenty of water.
      P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
      P308 + P313: IF exposed or concerned: Get medical advice/ attention.
      Signal WordDanger
      Storage class6.1C Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
      WGKWGK 3 highly hazardous to water
      Carcinogenic compounds and flammable compounds labelled "Highly toxic" or "Toxic": container F. Alkyl sulfates are carcinogenic; Take particular care to avoid inhalation and skin contact. To neutralize alkyl sulfates, add dropwise (from a dropping funnel) to concentrated ice-cool Ammonia solution (Cat.No. 105426) with vigorous stirring. Before placing in container D, check the pH with pH Universal indicator strips (Cat.No. 109535).
      Safety Information
      Categories of dangercarcinogenic
      Product Usage Statements
      Storage and Shipping Information
      StorageStore at +2°C to +30°C.
      Packaging Information
      Transport Information
      Declaration (railroad and road) ADR, RIDUN 3077 , 9, III
      Declaration (transport by air) IATA-DGRUN 3077 , 9, III
      Declaration (transport by sea) IMDG-CodeUN 3077 , 9, III, Marine Pollutant: P
      Supplemental Information
      Dye content (spectrophotometrically)≥ 75 %
      Identity (UV/VIS-Spectrum)passes test
      Absorption maximum λ max (ethanol 50 %)553 - 556 nm
      Spec. Absorptivity A 1%/1cm (λmax; 0.003 g/l; ethanol 50 %)1810 - 2430
      TLC-Testpasses test
      Loss on drying (110 °C)≤ 10 %
      Suitability for microscopypasses test