533196 Myogenic miRNA Inhibitor, Cpd1 - Calbiochem

533196
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Overview

Replacement Information

Key Specifications Table

Empirical Formula
C₃₃H₂₇NO₆

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      Glass bottle 10 mg
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      Description
      OverviewA cell-permeable tetrahydroquinolinedione[b]cyclobutene based compound that acts as a specific inhibitor of microRNA (miRNA)-221/222-myoD regulatory pathway. Inhibits the translation of myoD in C2C12 cells without changing the expression level of myoD mRNA, but reduces myoD protein expression levels. Shown to selectively inhibit miR-1 (IC50 = 2.5 µM), miR-133a and miR-206 (IC50 = 5 µM) in C2C12 cells. However, it does not affect the levels of non-myogenic miR-9, miR-25, miR-150, miR-25, and miR-214. Shown to inhibit the differentiation of C2C12 cells as indicated by the down-regulation of muscle creatine kinase, myosin heavy chain, and myoGenin levels in these cells.

      Please note that the molecular weight for this compound is batch-specific due to variable water content. Please refer to the vial label or the certificate of analysis for the batch-specific molecular weight. The molecular weight provided represents the baseline molecular weight without water.
      Catalogue Number533196
      Brand Family Calbiochem®
      Synonymstert-Butyl (5-(8a-methoxy-3,8-dioxo-2-phenyl-2a,3,8,8a-tetrahydrocyclobuta[b]naphthalen-1-yl)quinolin-8-yl) carbonate
      References
      ReferencesTan, S., et al. 2014. Chem. & Biol. 21, 1265.
      Product Information
      FormPale yellow solid
      Hill FormulaC₃₃H₂₇NO₆
      Chemical formulaC₃₃H₂₇NO₆
      Quality LevelMQ100
      Applications
      Biological Information
      Primary TargetmiR-221/22
      Primary Target IC<sub>50</sub>2.5 µ
      Purity≥98% by HPLC
      Physicochemical Information
      Cell permeableY
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage -20°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Myogenic miRNA Inhibitor, Cpd1 - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overview
      Tan, S., et al. 2014. Chem. & Biol. 21, 1265.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision26-June-2017 JSW
      Synonymstert-Butyl (5-(8a-methoxy-3,8-dioxo-2-phenyl-2a,3,8,8a-tetrahydrocyclobuta[b]naphthalen-1-yl)quinolin-8-yl) carbonate
      DescriptionA cell-permeable tetrahydroquinolinedione[b]cyclobutene based compound that acts as a specific inhibitor of microRNA (miRNA)-221/222-myoD regulatory pathway. Inhibits the translation of myoD in C2C12 cells without changing the expression level of myoD mRNA, but reduces myoD protein expression levels. Shown to selectively inhibit miR-1 (IC50 = 2.5 µM), miR-133a and miR-206 (IC50 = 5 µM) in C2C12 cells. However, it does not affect the levels of non-myogenic miR-9, miR-25, miR-150, miR-25, and miR-214. Shown to inhibit the differentiation of C2C12 cells as indicated by the down-regulation of muscle creatine kinase, myosin heavy chain, and myoGenin levels in these cells.
      FormPale yellow solid
      Intert gas (Yes/No) Packaged under inert gas
      Chemical formulaC₃₃H₂₇NO₆
      Purity≥98% by HPLC
      SolubilityDMSO (50 mg/ml)
      Storage -20°C
      Protect from light
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Toxicity Standard Handling
      ReferencesTan, S., et al. 2014. Chem. & Biol. 21, 1265.