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69831 Mouse Ig-Primer Set

69831
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      Description
      OverviewThe Ig-Primer Sets are designed for amplification of immunoglobulin (Ig) variable region cDNAs from human and mouse sources. Mammalian light- and heavy-chain Igs contain conserved regions adjacent to the hypervariable complementarity defining regions (CDRs). Appropriately designed oligonucleotide primer sets enable these regions to be specifically amplified using PCR (Larrick 1989, Jones 1991), after which the products may be sequenced directly or cloned. This powerful method enables rapid, precise analysis of hybridomas, myeloma cell progression and heterogeneity, idiotype networks, B-cell clonal ontogeny, and phylogenetic relationships.

      In practice, total RNA is isolated from a B-cell source, such as peripheral blood, lymph node(s), spleen, or hybridoma, and first-strand cDNA is prepared using reverse transcriptase. The primer sets are then used in PCR to specifically amplify the variable regions of light- and heavy-chain cDNAs.

      Please call Technical Service if you need more information regarding individual Ig-Primer Sets.

      Catalogue Number69831
      Brand Family Novagen®
      References
      ReferencesLarrick, J.W., et al. 1989. Biochem. Biophys. Res.
      Comm.
      160, 1250. Jones, S.T. and Bendig, M.M. 1991.
      Biotechnology 9, 88.
      Product Information
      500 pmolMu IgM VH 3ʹ-1 Primer
      500 pmolMu IgG VH 3ʹ-2 Primer
      500 pmolMu Ig VH 5ʹ-A Primer
      500 pmolMu Ig VH 5ʹ-B Primer
      100 pmolMu Ig VH 5ʹ-C Primer
      100 pmolMu Ig VH 5ʹ-D Primer
      100 pmolMu Ig VH 5ʹ-E Primer
      100 pmolMu Ig VH 5ʹ-F Primer
      500 pmolMu Igκ VL 3ʹ-1 Primer
      500 pmolMu Igκ VL 5ʹ-A Primer
      500 pmolMu Igκ VL 5ʹ-B Primer
      100 pmolMu Igκ VL 5ʹ-C Primer
      100 pmolMu Igκ VL 5ʹ-D Primer
      100 pmolMu Igκ VL 5ʹ-E Primer
      100 pmolMu Igκ VL 5ʹ-F Primer
      100 pmolMu Igκ VL 5ʹ-G Primer
      500 pmolMu Igλ VL 3ʹ-1 Primer
      500 pmolMu IgMλ VL 5ʹ-A Primer
      10 µlMouse Positive Control PCR Template
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Mouse Ig-Primer Set Certificates of Analysis

      TitleLot Number
      69831

      References

      Reference overview
      Larrick, J.W., et al. 1989. Biochem. Biophys. Res.
      Comm.
      160, 1250. Jones, S.T. and Bendig, M.M. 1991.
      Biotechnology 9, 88.

      Citations

      Title
    • Emi Hifumi, et al. (2008) Catalytic features and eradication ability of antibody light-chain UA15-L against Helicobacter pylori. Journal of Biological Chemistry 283, 899-907.
    • Anna S. Gardberg, et al. (2007) Molecular basis for passive immunotherapy of Alzheimer's disease. Procedings of the National Academy of Science 104, 15659-15664.
    • Kazuyo Toyoda, et al. (2007) Protein-bound 4-hydroxy-2-nonenal: an endogenous triggering antigen of anti-DNA response. Journal of Biological Chemistry 282, 25769-25778.
    • Manisha Goel, et al. (2004) Plasticity within the antigen-combining site may manifest as molecular mimicry in the humoral immune response. Journal of Immunology 173, 7358-7367.
    • Eric A. Grovender, et al. (2004) Single-chain antibody fragment-based Adsorbent for the extracorporeal removal of β2-microglobulin. Kidney International 65, 310-322.
    • Michael G. Rosenblum, et al. (2003) Design, expression, purification, and characterization, in vitro and in vivo, of an antimelanoma single-chain Fv antibody fused to the toxin gelonin. Cancer Research 63, 3995-4002.
    • Lingxun Duan, et al. (1994) Potent inhibition of human immunodeficiency virus type 1 replication by an intracellular anti-Rev single-chain antibody. Procedings of the National Academy of Science 91, 5075-5079.
    • User Protocols

      Title
      TB326 IgG Primer Sets

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      Categories

      Life Science Research > Genomic Analysis > DNA Preparation & Cloning > Primers