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530542 Mitosis Inhibitor II, Dosabulin Enantiomers Set - Calbiochem

A cell-permeable bridged bicycle heptene derived compound that can arrest mitosis (EC₅₀ = 1.23 µM) by inducing tubulin depolymerization. A negative control is included.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Mitosis Inhibitor II, (S)-Dosabulin and Negative Control Set, (1R,4S,5S)-methyl 4-(2-(benzo[b]thiophen-2-yl)phenyl)bicyclo[3.2.1]oct-2-ene-2-carboxylate

Primary Target: tubulin
530542
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Overview

Replacement Information

Key Specifications Table

Empirical Formula
C₂₄H₂₂O₂S

Pricing & Availability

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      Description
      OverviewA cell-permeable bridged bicycle heptene derived compound that can arrest mitosis (EC50 = 1.23 µM) by inducing tubulin depolymerization. Inhibits the growth of U2OS osteosarcoma cells (IC50 = 810 nM) and cause apoptotic cell death. Does neither affect the binding of vinblastine to tubulin nor does it displace colchicine, but is suggested to bind to a site that is vicinal or allosteric to it, which results in a reduced binding affinity for tubulin. The R-enantiomer of this compound lacks the ability to depolymemrize tubulin (>10 µM) and is included as a negative control.

      Please note that the molecular weight for this compound is batch-specific due to variable water content.
      Catalogue Number530542
      Brand Family Calbiochem®
      SynonymsMitosis Inhibitor II, (S)-Dosabulin and Negative Control Set, (1R,4S,5S)-methyl 4-(2-(benzo[b]thiophen-2-yl)phenyl)bicyclo[3.2.1]oct-2-ene-2-carboxylate
      References
      ReferencesIbbeson, B. M., et al. 2014. Nature Comm. 5, 3155.
      Product Information
      FormOff-white solid
      Formulation1 set contains 2 mg (S)-Dosabulin (+) (Cat. No. 5.30543.0001) and 2 mg (R)-Dosabulin Negative Control (-) (Cat. No. 5.30544.0001).
      Hill FormulaC₂₄H₂₂O₂S
      Chemical formulaC₂₄H₂₂O₂S
      ReversibleY
      Quality LevelMQ100
      Applications
      Biological Information
      Primary Targettubulin
      Purity≥98% by HPLC
      Physicochemical Information
      Cell permeableY
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage -20°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      References

      Reference overview
      Ibbeson, B. M., et al. 2014. Nature Comm. 5, 3155.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision27-March-2015 JSW
      SynonymsMitosis Inhibitor II, (S)-Dosabulin and Negative Control Set, (1R,4S,5S)-methyl 4-(2-(benzo[b]thiophen-2-yl)phenyl)bicyclo[3.2.1]oct-2-ene-2-carboxylate
      DescriptionA cell-permeable bridged bicycle heptene derived compound that can arrest mitosis (EC50 = 1.23 µM) by inducing tubulin depolymerization. Inhibits the growth of U2OS osteosarcoma cells (IC50 = 810 nM) and cause apoptotic cell death. Does neither affect the binding of vinblastine to tubulin nor does it displace colchicine, but is suggested to bind to a site that is vicinal or allosteric to it, which results in a reduced binding affinity for tubulin. The R-enantiomer of this compound lacks the ability to depolymemrize tubulin (>10 µM) and is included as a negative control.
      FormOff-white solid
      Formulation1 set contains 2 mg (S)-Dosabulin (+) (Cat. No. 5.30543.0001) and 2 mg (R)-Dosabulin Negative Control (-) (Cat. No. 5.30544.0001).
      Intert gas (Yes/No) Packaged under inert gas
      Chemical formulaC₂₄H₂₂O₂S
      Purity≥98% by HPLC
      SolubilityDMSO (50 mg/ml)
      Storage Protect from light
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Toxicity Standard Handling
      ReferencesIbbeson, B. M., et al. 2014. Nature Comm. 5, 3155.