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530390 Mitosis Inhibitor, AK301 - Calbiochem

530390
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Overview

Replacement Information

Key Specifications Table

Empirical Formula
C₁₉H₂₁ClN₂O₂

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      Glass bottle 10 mg
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      Description
      OverviewA cell permeable piperazine derivative that induces irreversible mitotic arrest in colon cancer cells (IC50 = 115 nM in HT29 cells) and increases their sensitivity to TNF-induced apoptosis (EC50 = 172 nM). Also shown to enhance FasL and TRAIL-induced caspase-3 activity in colon cancer cells. Binds to the chochicine-binding domain on b-tubulin with high affinity, although in a different orientation than cholchicine, and reduces tubulin polymerization with formation of multiple microtubule organizing centers with Aurora kinase A and g-tubulin. Induces G2/M arrest in both HT29 colon cancer cells and WI38 lung fibroblasts, however, this effect is more pronounced in cancer cells.
      Catalogue Number530390
      Brand Family Calbiochem®
      SynonymsTNF sensitizing agent
      References
      ReferencesChopra, A. S., et al. 2013. J. Bio. Chem. 289, 2978.
      Product Information
      FormWhite solid
      Hill FormulaC₁₉H₂₁ClN₂O₂
      Chemical formulaC₁₉H₂₁ClN₂O₂
      ReversibleY
      Quality LevelMQ100
      Applications
      Biological Information
      Primary Targettubulin
      Primary Target IC<sub>50</sub>115 nM
      Purity≥98% by HPLC
      Physicochemical Information
      Cell permeableY
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      References

      Reference overview
      Chopra, A. S., et al. 2013. J. Bio. Chem. 289, 2978.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision09-May-2014 JSW
      SynonymsTNF sensitizing agent
      DescriptionA cell permeable piperazine derivative that induces irreversible mitotic arrest in colon cancer cells (IC50 = 115 nM in HT29 cells) and increases their sensitivity to TNF-induced apoptosis (EC50 = 172 nM). Also shown to enhance FasL and TRAIL-induced caspase-3 activity in colon cancer cells. Binds to the chochicine-binding domain on b-tubulin with high affinity, although in a different orientation than cholchicine, and reduces tubulin polymerization with formation of multiple microtubule organizing centers with Aurora kinase A and g-tubulin. Induces G2/M arrest in both HT29 colon cancer cells and WI38 lung fibroblasts, however, this effect is more pronounced in cancer cells.
      FormWhite solid
      Intert gas (Yes/No) Packaged under inert gas
      Chemical formulaC₁₉H₂₁ClN₂O₂
      Purity≥98% by HPLC
      SolubilityDMSO (25 mg/ml)
      Storage +2°C to +8°C
      Protect from light
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Toxicity Standard Handling
      ReferencesChopra, A. S., et al. 2013. J. Bio. Chem. 289, 2978.