Millicell® Cell Culture Inserts

Optimized cell growth, attachment and differentiation

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Overview

Specifications

Ordering Information

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Millicell Standing Inserts Clear Sorting & Filtering
Catalog Numbericon Mediaicon Chemistryicon Pore Sizeicon Filtration Areaicon Device Configurationicon Pack Sizeicon
PI8P01250Isopore® Polycarbonate (PC) 8.0 µm 0.6 cm² 24-well plate 50 Show Pricing & Availability
PICM01250Biopore® Hydrophilic Polytetrafluoroethylene (PTFE) 0.4 µm 0.6 cm² 24-well plate 50 Show Pricing & Availability
PICM03050Biopore® Hydrophilic Polytetrafluoroethylene (PTFE) 0.4 µm 4.2 cm² 6-well plate 50 Show Pricing & Availability
PICM0RG50Biopore® Hydrophilic Polytetrafluoroethylene (PTFE) 0.4 µm 4.2 cm² 6-well plate 50 Show Pricing & Availability
PIHA01250MF-Millipore Mixed Cellulose Esters (MCE) 0.45 µm 0.6 cm² 24-well plate 50 Show Pricing & Availability
PIHA03050MF-Millipore Mixed Cellulose Esters (MCE) 0.45 µm 4.2 cm² 6-well plate 50 Show Pricing & Availability
PIHP01250Isopore® Polycarbonate (PC) 0.4 µm 0.6 cm² 24-well plate 50 Show Pricing & Availability
PIHP03050Isopore® Polycarbonate (PC) 0.4 µm 4.2 cm² 6-well plate 50 Show Pricing & Availability
PITP01250Isopore® Polycarbonate (PC) 3.0 µm 0.6 cm² 24-well plate 50 Show Pricing & Availability
PIXP01250Isopore® Polycarbonate (PC) 12.0 µm 0.6 cm² 24-well plate 50 Show Pricing & Availability

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Millicell Hanging Inserts Clear Sorting & Filtering
Catalog Numbericon Chemistryicon Pore Sizeicon Filtration Areaicon Device Configurationicon Pack Sizeicon
MCEP06H48Polyethylene Terephthalate (PET) 8.0 µm 4.5 cm² 6-well plate 48 Show Pricing & Availability
MCEP12H48Polyethylene Terephthalate (PET) 8.0 µm 1.1 cm² 12-well plate 48 Show Pricing & Availability
MCEP24H48Polyethylene Terephthalate (PET) 8.0 µm 0.3 cm² 24-well plate 48 Show Pricing & Availability
MCHT06H48Polyethylene Terephthalate (PET) 0.4 µm 4.5 cm² 6-well plate 48 Show Pricing & Availability
MCHT12H48Polyethylene Terephthalate (PET) 0.4 µm 1.1 cm² 12-well plate 48 Show Pricing & Availability
MCHT24H48Polyethylene Terephthalate (PET) 0.4 µm 0.3 cm² 24-well plate 48 Show Pricing & Availability
MCMP06H48Polyethylene Terephthalate (PET) 5.0 µm 4.5 cm² 6-well plate 48 Show Pricing & Availability
MCMP12H48Polyethylene Terephthalate (PET) 5.0 µm 1.1 cm² 12-well plate 48 Show Pricing & Availability
MCMP24H48Polyethylene Terephthalate (PET) 5.0 µm 0.3 cm² 24-well plate 48 Show Pricing & Availability
MCRP06H48Polyethylene Terephthalate (PET) 1.0 µm 4.5 cm² 6-well plate 48 Show Pricing & Availability
MCRP12H48Polyethylene Terephthalate (PET) 1.0 µm 1.1 cm² 12-well plate 48 Show Pricing & Availability
MCRP24H48Polyethylene Terephthalate (PET) 1.0 µm 0.3 cm² 24-well plate 48 Show Pricing & Availability
MCSP06H48Polyethylene Terephthalate (PET) 3.0 µm 4.5 cm² 6-well plate 48 Show Pricing & Availability
MCSP12H48Polyethylene Terephthalate (PET) 3.0 µm 1.1 cm² 12-well plate 48 Show Pricing & Availability
MCSP24H48Polyethylene Terephthalate (PET) 3.0 µm 0.3 cm² 24-well plate 48 Show Pricing & Availability

* For attachment-dependent cells, this membrane needs to be coated with an extra cellular matrix.

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Accessories Clear Sorting & Filtering
Catalog Numbericon Descriptionicon Pack Sizeicon
MERS00002Millicell ERS-2 Voltohmmeter 1 Show Pricing & Availability
MERSSTX01Replacement Electrodes, 1 pair 1 Show Pricing & Availability

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    Documentation

    Technical Info

    Title
    Millicell Organotypic Cell Culture Procedure

    References

    Reference overviewApplication
    Propagation of human embryonic stem cells in a microporous membrane-based indirect co-culture system
    Kelsey Albert, Steven Sheridan, Louise Laurent, Igor Ulitsky, Ron Shamir, Jeanne Loring, & Raj R. Rao
    Biochemical and Biophysical Research Communications  2010

    Astrocyte growth effects of vascular endothelial growth factor (VEGF) application to perinatal neocortical explants: Receptor mediation and signal transduction pathways
    Nina Mani, Alfia Khaibullina, Janette Krum and Jeffrey Rosenstein
    Experimental Neurology 192 (2005); 394-406  2005

    Cell Culture
    Subcellular localisation of recombinant a and g-synuclein
    Christian Specht, Cezar Tigaret, George Rast, Agnea Thalhammer, York Rudhard and Ralf Schoepfer
    Mol. Cell. Neurosci., 28 (2005); 326-334  2005

    Cell Culture
    Establishment of the organotypic model of amyotrophic lateral sclerosis from the SD rats' spinal cord
    Diao ZY, et. al,Beijing Da Xue Xue Bao. 2005 Apr 18;37(2):134-8. Chinese.
    Beijing Da Xue Xue Bao. 2005 Apr 18;37(2):134-8. Chinese.  2005

    Cell Culture
    Development of an in vitro blood-brain barrier model-cytotoxicity of mercury and aluminum.
    Toimela, T et. al.,Toxicol Appl Pharmacol. 2004 Feb 15;195(1):73-82.
    Toxicol Appl Pharmacol. 2004 Feb 15;195(1):73-82.  2004

    Morphological differentiation of bone marrow stromal cells into neuron-like cells after co-culture with hippocampal slice
    Abouelfetouh, Ayman, et al
    Brain Research (2004), Volume 1029, Issue 1 pp 114-119  2004

    Cell Culture
    Effect of sodium bicarbonate on extracellular pH, matrix accumulation, and morphology of cultured articular chondrocytes.
    Waldman SD, Couto DC, Omelon SJ, Kandel RA
    Tissue Engineering (2004), Nov-Dec;10(11-12):1633-40  2004

    Neural stem cells protect against glutamate-induced excitotoxicitiy and promote survival of injured motor neurons through the secretion of neurotrophic factors
    Jeronia Llado, Christine Haenggeli, Nicholas Maragakis, Evan Snyder and Jeffrey Rothstein
    Mol. Cell. Neurosci. , 27 (2004); 322-331  2004

    Cell Culture
    FGF-10 plays an essential role in the growth of the fetal prostate.
    Annemarie A. Donjacour, Axel A. Thomson and Gerald R. Cunha
    Developmental Biology 261 (1): 39-54  2003

    Cell Culture
    Changes in lymphokine receptor expression and fatty acid composition of phospholipids and triacylglycerols in rat adipocytes associated with lymph nodes following a transient immune challenge.
    J. D. Priddle, C. A. Mattacks, D. A. Sadler, H. A. MacQueen and C. M. Pond
    Cell Biology International 27 (1): 23-29  2003

    Cell Differentiation

    FAQ

    QuestionAnswer
    How long do the electrodes last?The electrodes will last at least 6 months with normal use and care, barring any physical damage. To prolong life of the electrodes, clean after every use and store dry.
    How should I store electrodes when not in use?When storing electrodes for long periods of time, wash the electrodes with Milli-Q water or equivalent to remove salts and proteins, then store dry. For short term storage electrodes can be stored in a buffered solution. Make sure the electrode cable plug is connected to the electrode port on the Millicell ERS meter so that the system is internally short-circuited and electrode symmetry is maintained.
    I am coating the Millicell CM with Rat Tail collagen according to your instructions and sometimes I am seeing an empty ring at the periphery of the insert where the collagen has not filled in and an empty "hole" at the center where there is no coating either. How can I avoid this from happening in the future?If you are using Type 1 collagen, it should be filter sterilized in non-denaturing alcohol prior to use in the Millicell. Also, try looking at the insert while you are coating it and tap/rotate it to enhance uniform liquid coating. The entire membrane should go clear which indicates uniform wetting/coverage of the Millicell. Also, be sure to use at least minimum volumes of 50ul for the 12mm and 350 ul per 30 mm.
    I have seeded cells on the Millicell and they are detaching from the membrane, what do I do to correct this?Make sure that the medium on the outside of the insert is lower than the level of medium on the inside of the insert. Also, if it is important to remember that certain cells lines, i.e. attachment dependent cells, often require pretreatment of the Millicell membrane in order to enhance attachment. This is usually accomplished through ECM coating...especially in the case of Millicell CM where coating is always required. Too much feeding of unconditioned media can also cause detachment in some cell types, e.g. caco-2.
    Is it necessary to treat the Millicell CM with an ECM coating if I am doing organotypic culture ?In general, it is not required that you treat the Millicell CM with an ECM coating when doing organotypic culture but this is dependent on the type of tissue being cultured and the procedure being followed. In Tech Note TN062 , we outline a procedure for organotypic culture that uses a pretreatment of the Millicell CM with poly-ornithine ( an attachment factor). Other references cited in this paper do not use this precoating procedure. When evaluating whether or not to use an attachment factor in your experiment, it is best to use as many resources as possible and decide based upon the specific experimental parameters in your situation.
    What cell types can be used with the Millicell ERS?The MERS will only give meaningful readings on a cell line that is know to form tight junctions e.g. epithelial cells. We strongly recommend using the ERS for those cell lines where published values exist for the expected resistance. Many cell types do not have a large enough resistance to use this technique.
    What is an ECM and why should it be used?ECM is extracellular matrix material and is used to coat certain Millicells ( e.g. Millicell CM) to enhance cellular attachment. Normally, attachment dependent cells cannot readily bind to the Biopore membrane so coating the Millicell with collagen or other ECM material will render the filter surface suitable for cell attachment. Please note: the Millicell PCF is tissue culture treated and should not require additional coating. In general, the Millicell HA does not require coating with an ECM and will support direct cell attachment.
    What Volume of media should be used on the inside and outside of the 12 mm and 30mm Millicell cell culture insert devices?Inside and outside liquid heights are critical and must be adjusted until equal. The suggested volumes below can vary due to plate well volume variations allowed by different cell culture plate manufacturers.

    12 mm inserts:
    Inside: 0.4 mL
    Outside: 0.6 mL
    Why do the electrodes need to be replaced every six months?In general, electrodes have a 6 month lifetime if they are used continuously. The electrodes have a silver-silver chloride pellete that is depleted everytime it is used. It is the silver silver chloride pellete that creates the potential.
    What voltage value can I expect for my cell type?We DO NOT recommend using this function on the instrument. The Millicell-ERS will make voltage readings on only a few kinds of high-voltage generating cells (for example, it will not work on MDCK cells). We do have information on epithelial cells which typically have voltage values of 0-30mV.

    User Guides

    Title
    Millicell 24 and Millicell 96
    Millicell Culture Plate Inserts
    Millicell Hanging Cell Culture Inserts
    Millicell-CM Low Height Culture Plate Inserts User Guide
    Millicell-ERS User Guide
    Millicell® Hanging Cell Culture Inserts