Millicell® Cell Culture Inserts
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Overview
Specifications
Ordering Information
Documentation
Technical Info
| Title |
|---|
| Millicell Organotypic Cell Culture Procedure |
References
| Reference overview | Application |
|---|---|
| Propagation of human embryonic stem cells in a microporous membrane-based indirect co-culture system Kelsey Albert, Steven Sheridan, Louise Laurent, Igor Ulitsky, Ron Shamir, Jeanne Loring, & Raj R. Rao Biochemical and Biophysical Research Communications 2010 | |
| Astrocyte growth effects of vascular endothelial growth factor (VEGF) application to perinatal neocortical explants: Receptor mediation and signal transduction pathways Nina Mani, Alfia Khaibullina, Janette Krum and Jeffrey Rosenstein Experimental Neurology 192 (2005); 394-406 2005 | Cell Culture |
| Subcellular localisation of recombinant a and g-synuclein Christian Specht, Cezar Tigaret, George Rast, Agnea Thalhammer, York Rudhard and Ralf Schoepfer Mol. Cell. Neurosci., 28 (2005); 326-334 2005 | Cell Culture |
| Establishment of the organotypic model of amyotrophic lateral sclerosis from the SD rats' spinal cord Diao ZY, et. al,Beijing Da Xue Xue Bao. 2005 Apr 18;37(2):134-8. Chinese. Beijing Da Xue Xue Bao. 2005 Apr 18;37(2):134-8. Chinese. 2005 | Cell Culture |
| Development of an in vitro blood-brain barrier model-cytotoxicity of mercury and aluminum. Toimela, T et. al.,Toxicol Appl Pharmacol. 2004 Feb 15;195(1):73-82. Toxicol Appl Pharmacol. 2004 Feb 15;195(1):73-82. 2004 | |
| Morphological differentiation of bone marrow stromal cells into neuron-like cells after co-culture with hippocampal slice Abouelfetouh, Ayman, et al Brain Research (2004), Volume 1029, Issue 1 pp 114-119 2004 | Cell Culture |
| Effect of sodium bicarbonate on extracellular pH, matrix accumulation, and morphology of cultured articular chondrocytes. Waldman SD, Couto DC, Omelon SJ, Kandel RA Tissue Engineering (2004), Nov-Dec;10(11-12):1633-40 2004 | |
| Neural stem cells protect against glutamate-induced excitotoxicitiy and promote survival of injured motor neurons through the secretion of neurotrophic factors Jeronia Llado, Christine Haenggeli, Nicholas Maragakis, Evan Snyder and Jeffrey Rothstein Mol. Cell. Neurosci. , 27 (2004); 322-331 2004 | Cell Culture |
| FGF-10 plays an essential role in the growth of the fetal prostate. Annemarie A. Donjacour, Axel A. Thomson and Gerald R. Cunha Developmental Biology 261 (1): 39-54 2003 | Cell Culture |
| Changes in lymphokine receptor expression and fatty acid composition of phospholipids and triacylglycerols in rat adipocytes associated with lymph nodes following a transient immune challenge. J. D. Priddle, C. A. Mattacks, D. A. Sadler, H. A. MacQueen and C. M. Pond Cell Biology International 27 (1): 23-29 2003 | Cell Differentiation |
FAQ
| Question | Answer |
|---|---|
| Why do the electrodes need to be replaced every six months? | In general, electrodes have a 6 month lifetime if they are used continuously. The electrodes have a silver-silver chloride pellete that is depleted everytime it is used. It is the silver silver chloride pellete that creates the potential. |
| What voltage value can I expect for my cell type? | We DO NOT recommend using this function on the instrument. The Millicell-ERS will make voltage readings on only a few kinds of high-voltage generating cells (for example, it will not work on MDCK cells). We do have information on epithelial cells which typically have voltage values of 0-30mV. |
| What is the porosity of the Millicell Culture Plate Inserts? | The porosity of the Millicell Insert membranes are as follows: HATF - approx 80% CM - approx 80% PC and PCF - approx 10% |
| What is the thickness of the Millicell-CM membrane? | The thickness of the Millicell-CM memnbrane is approximately 50 um. |
| What is the difference between Millicell PC and PCF? | Both Millicells have polycarbonate membranes and are frequently used in chemotaxis and transport studies. PC's are recommended ONLY for suspension cell culture. PCF's are tissue culture treated and therefore recommended for attachment dependent cell culture. |
| What is the advantage of using Millicell for cell culture applications ? | Studies have shown that cells actually grow better on microporous membranes than on the artificially smooth surfaces of standard cell culture plasticware. We have found that cells grown on Millicell Cell Culture Plate Inserts produce higher cell densities and greater differentiation than cells grown in standard setups. Cells can be constantly bathed and fed on both their apical and basolateral surfaces and ions and molecules can pass freely across the membrane. Millicells also offer the researcher a more humane approach to animal testing since cell culturing with the units can offer alternatives to live animal studies for many toxicological studies. |
| Are the Millicell PCF inserts tissue culture treated on both sides of the membrane? | Yes, they are tissue culture treated on both sides of the membrane. This allows for attachment- dependent cell culture on both sides of the membrane. |
| Are the Millicell units sterile and, if so, how are they sterilized? | The Millicell units are sold sterile in individual blister packs. The HA and CM units are ETO sterilized and the PC and PCF are Gamma Irradiated. |
| Can cells grown on Isopore (polycarbonate) membrane be viewed under a microscope? | Yes, we can observe the cells seeding on the membrane of pore size 1 um. |
| Can I make membrane potential measurements with the Millicell-ERS? | Yes, membrane potential measurements can be made with the ERS. Short circuit potential, however, cannot be done. |