|Propagation of human embryonic stem cells in a microporous membrane-based indirect co-culture system|
Kelsey Albert, Steven Sheridan, Louise Laurent, Igor Ulitsky, Ron Shamir, Jeanne Loring, & Raj R. Rao
Biochemical and Biophysical Research Communications 2010
|Effects of cimetidine on the biological behaviors of human gastric cancer cells|
Jiang CG, Liu FR, Xu HM, Wu T, Gao J.
Zhonghua Yi Xue Za Zhi. 2006 Jul 11;86(26):1813-6 2006
|Overexpression of TIMP-1 mediated by recombinant adenovirus in hepatocellular carcinoma cells inhibits proliferation and invasion in vitro|
Xia D, Yan LN, Xie JG, Tong Y, Yan ML, Wang XP, Zhang MM, Zhao LY.
Hepatobiliary Pancreat Dis Int. 2006 Aug;5(3):409-15. 2006
|Glucose utilization by the retinal pigment epithelium: evidence for rapid uptake and storage in glycogen, followed by glycogen utilization.|
Senanayake P, Calabro A, Hu JG, Bonilha VL, Darr A, Bok D, Hollyfield JG.
Exp Eye Res. 2006 Aug;83(2):235-46. Epub 2006 May 11. 2006
|A comparison of polarized and non-polarized human endometrial monolayer culture systems on murine embryo development.|
Baghaban Eslami Nejad MR, Rezazadeh Valojerdi M, Kazemi Ashtiani S.
J Exp Clin Assist Reprod. 2005 Apr 19;2(1):7. 2005
|Millicell 24 and Millicell 96|
|Millicell Culture Plate Inserts|
Millicell cell culture insert plates are a patented device designed to support cell growth and differentiation of endothelial and epithelial cell lines, including CaCo-2.
The Millicell device is available with a 0.4 µm PCF and a variety of PET membrane pore sizes. The plate is designed for maximum user convenience and incorporates features such as apical assist for easier pipetting and basolateral access. To reduce the risk of monolayer contamination, the membrane plates have feet to elevate the plate above the work surface when disassembled from the feeder tray. Tear drop-shaped receiver wells eliminate air bubbles as plates are assembled.
Millicell plate are optimized to grow and sustain high integrity cell monolayers. Cells grown on Millicell plate membranes grow better than on plastic because the cells are nourished from both the apical and basolateral sides. Cell growth and function more closely mimic in vivo. Millicell plates are designed for analysis as well as cell growth and can be used manually or with automated cell seeding, feeding and washing systems
With this complete system, you can grow, feed, and analyze cells in one membrane-bottom plate. The plate can be used with either a single-well or a multi-well feeding tray. At the time of transport analysis, the plate is simply transferred to a multi-well transport tray for analysis.
This streamlined design enhances functionality with:
- Seed-and-feed systems
- Automated liquid handling systems and span pipettes
- Basolateral and apical access to cells
- Transepithelial electrical resistance (TEER) measurement systems
In-plate differentiation of embryonic stem cells and phenotype analysis
Neuron differentiation of embryonic stem cells in Millicell 24 1 µm PET filter plates. Murine embryonic stem cells were formed into suspended embryoid bodies (EBs) then transferred to 1 µm PET Millicell 24 for attachment and differentiation. Neural differentiation after retinoic acid treatment of attached EBs was confirmed by anti-neurofilament immunofluoresence. (Insert: Inverted phase contrast imaging through membrane of live EBs in media.)
Demonstrated transport and efflux studies with integral differentiated Caco-2 monolayers
Differentiation of 21-day Caco-2 monolayer by efflux pump expression. Anti-MDR1 immunohistochemistry confirms p-glycoprotein (MDR1) expression of Caco-2 monolayer grown in Millicell 24 with 1 µm PET membrane.
|High pore density 0.4µm PCF membrane||Grows well differentiated Caco-2 cells with no grow through|
|1.0µm Transparent PET membrane||Allows for visualization of live cells using microscopy|
|Apical and basolateral access holes||Ease of sample access when performing sequence time transport analysis|
|Apical assist channel||Prevent monolayer disruption and membrane damage while performing manual assays|
|Filter plate has feet||Do not compromise sterility if set on bench|
Choice of membranes lets you optimize for your application
Millicell cell culture plates can be used for a wide variety of applications from cell surface receptor assays, to in vitro toxicology. The device can also be used to measure drug transport and other cell based assays. See the applications guide for a complete listing.
The plates are available with thin-film polycarbonate (PCF) membrane or polyester (PET), which is a microscopically-transparent membrane.
Cell Growth, Feeding, Cell Analysis