MAB2300X Milli-Mark™ FluoroPan Neuronal Marker - Alexa488 conjugated

MAB2300X
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, M ICC, IF M AlexaFluor®488 Monoclonal Antibody
      Description
      Catalogue NumberMAB2300X
      Trade Name
      • Neuro-Chrom
      DescriptionMilli-Mark™ FluoroPan Neuronal Marker - Alexa488 conjugated
      Background InformationAntibodies to neuronal proteins have become critical tools for identifying neurons and discerning morphological characteristics in culture and complex tissue. While the labeling from classic histological techniques, such as Golgi staining, and modern molecular approaches, such as GFP constructs, yield excellent cytoarchitectural detail, these approaches are technically challenging and impractical for many neuroscience research needs. Neuron-specific antibodies are convenient precision tools useful in revealing cytoarchitecture, but are limited to the protein target distribution within the neuron, which may differ greatly from nucleus to soma to dendrite and axon. To achieve as complete a morphological staining as possible across all parts of neurons, Millipore previously developed a monoclonal antibody blend (Catalog No. MAB2300) that reacts against key somatic, nuclear, dendritic, spine, and axonal proteins distributed across the pan-neuronal architecture. Now Millipore has formulated MAB2300X, an Alexa 488 conjugated version of the pan-neuronal antibody blend MAB2300 to facilitate immunofluorescence studies. Each antibody within the blend is conjugated to Alexa 488 therefore enabling detection of an entire neuron with only one microscopic emission channel. MAB2300X has been validated in diverse fixations, cell culture, and immunohistochemistry protocols, giving researchers a convenient and specific qualitative and quantitative tool for studying neuronal morphology. High morphological resolution has been achieved using MAB2300x including distral dentrites and spines.
      References
      Product Information
      FormatAlexaFluor®488
      Control
      • Cortical neurons
      PresentationBlended monoclonal antibody cocktail in PBS with 0.05% NaN3
      Quality LevelMQ100
      Applications
      ApplicationMilli-Mark FluoroPan Neuronal Marker -Alexa488 conjugated is an antibody targeting the FluoroPan Neuronal Marker protein, validated for use in ICC & IF.
      Key Applications
      • Immunocytochemistry
      • Immunofluorescence
      Application NotesImmunocytochemistry:

      1. Culture neurons as desired. When ready, gently wash with PBS and fix cells with 4% paraformaldehyde in PBS, 30min at room temp (RT).
      2. Wash with PBS, 3 x 5min each, at RT and block with blocking buffer (1% BSA, 5% Serum, 0.2% Triton X in PBS) for 1 hr at RT.
      3. Incubate cells with Milli-Mark FluoroPan Neuronal Marker (Mouse IgG conjugated with Alexa 488) for 2hr at RT (dilute antibody in blocking buffer).
      4. Wash with PBS, 3 x 5min each, and cover slip with LIGHT DIAGNOSTICS Mounting Fluid (Catalog No. #5013). View using fluorescent microscope with FITC filter.
      Biological Information
      EpitopeWhole Neuron Marker
      HostMouse
      SpecificityMAB2300X is specific to axons (neurites), dendrites, spines, nucleus, and the cell body of neurons.
      IsotypeIgG1
      Species Reactivity
      • Human
      • Rat
      • Mouse
      Species Reactivity NoteRat, Mouse, and Human. Reactivity with other species has not been determined.
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely tested on cortical neurons in Immunocytochemistry 1:150 - 1:100.



      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C for up to 6 months from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Milli-Mark™ FluoroPan Neuronal Marker - Alexa488 conjugated SDS

      Title

      Safety Data Sheet (SDS) 

      Milli-Mark™ FluoroPan Neuronal Marker - Alexa488 conjugated Certificates of Analysis

      TitleLot Number
      Milli-Mark FluoroPan Neuronal Marker - NG1752517NG1752517
      Milli-Mark FluoroPan Neuronal Marker - LV164182LV164182
      Milli-Mark FluoroPan Neuronal Marker - NG1609156NG1609156
      Milli-Mark FluoroPan Neuronal Marker - NG1657398NG1657398
      Milli-Mark FluoroPan Neuronal Marker - NG1667896NG1667896
      Milli-Mark FluoroPan Neuronal Marker - NG1820227NG1820227
      Milli-Mark FluoroPan Neuronal Marker - NG1894428NG1894428
      Neuro-Chrom FluoroPan Neuronal Marker - 21166942116694
      Neuro-Chrom FluoroPan Neuronal Marker - 20398332039833
      Neuro-Chrom FluoroPan Neuronal Marker - 21612182161218

      References

      Reference overviewPub Med ID
      IGF-1 enhances cell proliferation and survival during early differentiation of mesenchymal stem cells to neural progenitor-like cells.
      Huat, TJ; Khan, AA; Pati, S; Mustafa, Z; Abdullah, JM; Jaafar, H
      BMC neuroscience  15  91  2014

      Show Abstract
      25047045 25047045
      Morphological, immunocytochemical, and functional characterization of esophageal enteric neurons in primary culture.
      Dong, H; Jiang, Y; Srinivasan, S; Mittal, RK
      American journal of physiology. Gastrointestinal and liver physiology  305  G129-38  2013

      Show Abstract
      23660501 23660501

      Brochure

      Title
      AXIS: Axon Investigation System

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies