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115943 Methylene blue (C.I.52015)

for microscopy Certistain®

Methylene blue (C.I.52015) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Methylthioninium chloride, 3,7-Bis(dimethylamino)phenothiazinium chloride, Tetramethylthionine chloride

CAS #: 61-73-4 EC Number: 200-515-2 Molar Mass: 319.86 g/mol (anhydrous) Chemical Formula: C₁₆H₁₈ClN₃S * x H₂O (x=2-3) Hill Formula: C₁₆H₁₈ClN₃S * x H₂O
115943
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Overview

Replacement Information

Key Specifications Table

CAS #EC NumberHill FormulaChemical FormulaMolar Mass
61-73-4200-515-2C₁₆H₁₈ClN₃S * x H₂OC₁₆H₁₈ClN₃S * x H₂O (x=2-3)319.86 g/mol (anhydrous)

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      		 25 g
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          Description
          Catalogue Number115943
          SynonymsMethylthioninium chloride, 3,7-Bis(dimethylamino)phenothiazinium chloride, Tetramethylthionine chloride
          DescriptionMethylene blue (C.I.52015)
          OverviewMethylene blue (C.I. 52015) for microscopy Certistain® is a dry dye that is used in bacteriology for an overview stain and as counterstaining for Ziehl-Neelsen method in sample material of human origin. Bacteria appear blue. The dye has a further important application in hematology: Methylene blue and eosin are the main components of many staining solutions and dye mixtures. Certistain® is the product group for seleceted high quality dyes that fulfill all quality requirements of IVD and CE registration. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
          References
          Product Information
          CAS number61-73-4
          EC number200-515-2
          Hill FormulaC₁₆H₁₈ClN₃S * x H₂O
          Chemical formulaC₁₆H₁₈ClN₃S * x H₂O (x=2-3)
          Molar Mass319.86 g/mol (anhydrous)
          HS Code3212 90 00
          Structure formula ImageStructure formula Image
          Quality LevelMQ400
          Applications
          Applicationfor microscopy Certistain®
          Biological Information
          Physicochemical Information
          Melting Point180 °C (decomposition)
          pH value3 (10 g/l, H₂O, 20 °C)
          Bulk density400 - 600 kg/m3
          Solubility50 g/l
          Dimensions
          Materials Information
          Toxicological Information
          LD 50 oralLD50 Rat 1180 mg/kg
          Safety Information according to GHS
          Hazard Pictogram(s) Hazard Pictogram(s)
          Hazard Statement(s)H302: Harmful if swallowed.
          Precautionary Statement(s)P264: Wash skin thoroughly after handling.
          P270: Do not eat, drink or smoke when using this product.
          P301 + P312: IF SWALLOWED: Call a POISON CENTER/doctor if you feel unwell.
          P501: Dispose of contents/ container to an approved waste disposal plant.
          Signal WordWarning
          Storage class10 - 13 Other liquids and solids
          WGKWGK 2 obviously hazardous to water
          Disposal3
          Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.
          Safety Information
          Categories of dangerharmful
          Product Usage Statements
          Storage and Shipping Information
          StorageStore at +2°C to +30°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications
          Dye content (spectrophotometrically)≥ 82 %
          Identity
          - Chloride
          passes test
          - UV/VIS-Spectrumpasses test
          Absorption maximum λ max (ethanol 50 %)660 - 665 nm
          Spec. Absorptivity A 1%/1cm (λmax; 0.003 g/l; ethanol 50 %)2250 - 2750
          TLC-Testpasses test
          Loss on drying (110 °C)10 - 15 %
          Suitability for microscopypasses test

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          IVD/OEM Materials & Reagents > Microscopy > Dyes for Microscopy > Certistain® Dyes
          Reagents, Chemicals and Labware > Inorganic Reagents > Safety Products and General Applications > Indicators > Redox Indicators