475989 MTT - CAS 298-93-1 - Calbiochem

475989
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Overview

Replacement Information

Key Specifications Table

Empirical FormulaCAS #
C₁₈H₁₆BrN₅S 298-93-1

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475989-10GM
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      Plastic ampoule 10 gm
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      475989-1GM
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          Description
          OverviewMembrane-permeable yellow dye that is reduced by mitochondrial reductases in living cells to form the dark blue product, MTT-formazan. Spectrophotometric measurement of MTT-formazan at 540 or 570 nm allows quantitation of cell viability. Used as a direct indicator of cytotoxicity (such as for screening cancer drugs) and apoptosis. An electron acceptor used for studying NADP-dependent dehydrogenases.
          Catalogue Number475989
          Brand Family Calbiochem®
          Synonyms3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium Bromide, Thiazole Blue
          References
          ReferencesLiu, Y., et al. 1997. J. Neurochem. 69, 581.
          Abdallah, M.A. 1980. Eur. J. Biochem. 112, 331.
          Product Information
          CAS number298-93-1
          ATP CompetitiveN
          FormYellow powder
          Hill FormulaC₁₈H₁₆BrN₅S
          Chemical formulaC₁₈H₁₆BrN₅S
          ReversibleN
          Structure formula ImageStructure formula Image
          Quality LevelMQ100
          Applications
          Biological Information
          Primary TargetUsed as a direct indicator of cytotoxicity and apoptosis.
          Purity≥98% by HPLC
          Physicochemical Information
          Cell permeableY
          ContaminantsSulfated ash: ≤0.5%; heavy metals: ≤0.001%
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          RTECSXF8060000
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Harmful & Carcinogenic / Teratogenic
          Storage +15°C to +30°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C.). Stock solutions are stable for up to 1 month at -20°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          MTT - CAS 298-93-1 - Calbiochem SDS

          Title

          Safety Data Sheet (SDS) 

          MTT - CAS 298-93-1 - Calbiochem Certificates of Analysis

          TitleLot Number
          475989

          References

          Reference overview
          Liu, Y., et al. 1997. J. Neurochem. 69, 581.
          Abdallah, M.A. 1980. Eur. J. Biochem. 112, 331.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision06-December-2019 JSW
          Synonyms3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium Bromide, Thiazole Blue
          DescriptionMembrane-permeable yellow dye that is reduced by mitochondrial reductases in living cells to form the dark blue product, MTT-formazan. Spectrophotometric measurement of MTT-formazan at 540 or 570 nm allows quantitation of cell viability. Used as a direct indicator of cytotoxicity (such as for screening cancer drugs) and apoptosis. An electron acceptor used for studying NADP-dependent dehydrogenases.
          FormYellow powder
          CAS number298-93-1
          RTECSXF8060000
          Chemical formulaC₁₈H₁₆BrN₅S
          Structure formulaStructure formula
          Purity≥98% by HPLC
          ContaminantsSulfated ash: ≤0.5%; heavy metals: ≤0.001%
          SolubilityH₂O (5 mg/ml)
          Storage +15°C to +30°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C.). Stock solutions are stable for up to 1 month at -20°C.
          Toxicity Harmful & Carcinogenic / Teratogenic
          ReferencesLiu, Y., et al. 1997. J. Neurochem. 69, 581.
          Abdallah, M.A. 1980. Eur. J. Biochem. 112, 331.