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ASK10 MMP/TIMP Family Antibody Sampler Kit I

ASK10
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ASK10-1EA
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      Description
      OverviewContains 20 µg of each antibody. Includes Anti-MMP-1 (Cat. No. IM35), Anti-MMP-2 (Cat. No. IM33), Anti-MMP-3 (Cat. No. IM36), Anti-MMP-9 (Cat. Nos. IM09 and IM37), Anti-TIMP-1 (Cat. No. IM32), and Anti-TIMP-2 (Cat. No. IM11). Please see individual product descriptions for more information.
      Catalogue NumberASK10
      Brand Family Calbiochem®
      Application Data


      References
      ReferencesHöyhtyä, M., et al. 1994. Int. J. Cancer. 56, 500.
      Cottam, D. W. and Rees, R. C. 1993. Intl J. Oncol. 2, 861.
      Fujimoto, N., et al. 1993. Clinica Chimica Acta. 221, 91.
      Nakajima, M., et al. 1993. Cancer Res. 53, 5802.
      Stetler-Stevenson, W. G., et al. 1993. FASEB J. 7, 1434.
      Zhang, J., et al. 1993. Clinica Chimica Acta. 219, 1.
      Zucker, S., et al. 1993. Cancer Res. 53, 140.
      Obata, K., et al. 1992. Clinica Chimica Acta. 211, 59.
      Woessner, J. F. 1991. FASEB J. 5, 2145.
      Liotta, L. A. and Stetler-Stevenson, W. G. 1990. in Seminars in Cancer Biology, ed. M. M. Gottesman. Vol.1(2); 99-102.
      Kodama, S., et al. 1987. Collagen Rel. Res. 7, 341.
      Product Information
      DeclarationManufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.
      FormLiquid
      Formulation20 µg of each antibody in 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.4.
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesMMP-9 (Ab-2) Frozen and Paraffin Sections Aikawa, M, et al. 1998. Circ. Res. 83, 1015. MMP-2 (Ab-3) Paraffin Sections: Tsuchiya, Y., et al. 1994. Int. J. Cancer. 56, 46. MMP-1 (Ab-1), MMP-2 (Ab-3), and MMP-3 (Ab-1) Frozen Sections Nakagawa, T., et al. 1994. J. Neurosurg. 81, 69. MMP-1 (Ab-1) Paraffin Sections: Zhang, J., et al. 1993. Clinica Chimica Acta. 219, 1. MMP-2 (Ab-3) Paraffin Sections Nomura H., et al. 1996. Int. J. Cancer 69, 9. Obata, K., et al. 1992. Clinica Chimica Acta. 211, 59. (called clone B) MMP-9 (Ab-1) Enzyme Inhibition: Ramos-DeSimone, N., et al. 1993. Hybridoma 12, 349. MMP-9 (Ab-1) and MMP-9 (Ab-2) Immunoprecipitation Ramos-DeSimone, N. and French, D. L. 1994. Ann. NY Acad. Sci. 732, 469. TIMP-1 (Ab-1) Imunohistochemistry Okada, Y., et al. 1990. Virchows Arch. B Cell Pathol. 59, 305. TIMP-2 (Ab-1) Immunoprecipitation and Frozen Sections Höyhtyä, M., et al. 1994. Int. J. Cancer. 56, 500.
      Application NotesFrozen Sections (see characteristics below)
      Immunoblotting (see characteristics below)
      Immunoprecipitation (see characteristics below)
      Paraffin Sections (see characteristics below)
      Application CommentsFor an MMP-1 or MMP-3 positive control, incubate HT-1080 cells for 2 hours at 37°C in serum-free media containing 100 nM PMA. Collect and centrifuge medium; concentrate as necessary. Store in aliquots at -20°C. When staining paraffin sections for MMP-1, pretreatment with heat, saponin, or trypsin can be used, but a pressure cooker generally gives the best results. Anti-MMP-9 (Ab-1) recognizes both the latent and active forms of MMP-9 under non-reducing conditions but only the latent form under reducing conditions. Anti-MMP-9 (Ab-1) will inhibit MMP-9 enzymatic activity. Anti-MMP-9 (Ab-2) recognizes only the latent form of MMP-9 (non-reducing or reducing conditions). Anti-TIMP-1 (Ab-1) will react with TIMP-1 and MMP/TIMP-1 complexes. Antibodies should be titrated for optimal results in individual systems.
      Biological Information
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      MMP/TIMP Family Antibody Sampler Kit I Certificates of Analysis

      TitleLot Number
      ASK10

      References

      Reference overview
      Höyhtyä, M., et al. 1994. Int. J. Cancer. 56, 500.
      Cottam, D. W. and Rees, R. C. 1993. Intl J. Oncol. 2, 861.
      Fujimoto, N., et al. 1993. Clinica Chimica Acta. 221, 91.
      Nakajima, M., et al. 1993. Cancer Res. 53, 5802.
      Stetler-Stevenson, W. G., et al. 1993. FASEB J. 7, 1434.
      Zhang, J., et al. 1993. Clinica Chimica Acta. 219, 1.
      Zucker, S., et al. 1993. Cancer Res. 53, 140.
      Obata, K., et al. 1992. Clinica Chimica Acta. 211, 59.
      Woessner, J. F. 1991. FASEB J. 5, 2145.
      Liotta, L. A. and Stetler-Stevenson, W. G. 1990. in Seminars in Cancer Biology, ed. M. M. Gottesman. Vol.1(2); 99-102.
      Kodama, S., et al. 1987. Collagen Rel. Res. 7, 341.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision03-December-2007 JSW
      ApplicationFrozen Sections (see characteristics below)
      Immunoblotting (see characteristics below)
      Immunoprecipitation (see characteristics below)
      Paraffin Sections (see characteristics below)
      Application Data


      DescriptionContains 20 µg of each antibody. Includes Anti-MMP-1 (Cat. No. IM35), Anti-MMP-2 (Cat. No. IM33), Anti-MMP-3 (Cat. No. IM36), Anti-MMP-9 (Cat. Nos. IM09 and IM37), Anti-TIMP-1 (Cat. No. IM32), and Anti-TIMP-2 (Cat. No. IM11). Please see individual product descriptions for more information. The antibodies to MMP-1, MMP-2, and MMP-3 recognize both latent and active forms.
      BackgroundMatrix metalloproteinases (MMPs) are a family of enzymes that are responsible for the degradation of extracellular matrix components such as collagen, laminin and proteoglycans. All MMPs share: sequence homology, a catalytic mechanism dependent upon a zinc ion at the active center, ability to cleave one or more extracellular matrix components, secretion as zymogens which are activated by removal of an approximately 10 kDa segment from the N-terminus, and regulation by endogenous inhibitors. These enzymes are involved in normal physiological processes as well as in metastasis. MMP-2 and MMP-9 are members of the gelatinase family and elevated levels may be correlated with metastatic potential. MMP-1 is also known as interstitial collagenase and MMP-3 as stromelysin-1. Activation and activity of the MMP's are regulated by the TIMP (tissue inhibitors of metalloproteinase) family members. TIMP-1 preferentially regulates MMP-9 and TIMP-2 regulates MMP-2.
      FormLiquid
      Formulation20 µg of each antibody in 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.4.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsFor an MMP-1 or MMP-3 positive control, incubate HT-1080 cells for 2 hours at 37°C in serum-free media containing 100 nM PMA. Collect and centrifuge medium; concentrate as necessary. Store in aliquots at -20°C. When staining paraffin sections for MMP-1, pretreatment with heat, saponin, or trypsin can be used, but a pressure cooker generally gives the best results. Anti-MMP-9 (Ab-1) recognizes both the latent and active forms of MMP-9 under non-reducing conditions but only the latent form under reducing conditions. Anti-MMP-9 (Ab-1) will inhibit MMP-9 enzymatic activity. Anti-MMP-9 (Ab-2) recognizes only the latent form of MMP-9 (non-reducing or reducing conditions). Anti-TIMP-1 (Ab-1) will react with TIMP-1 and MMP/TIMP-1 complexes. Antibodies should be titrated for optimal results in individual systems.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesHöyhtyä, M., et al. 1994. Int. J. Cancer. 56, 500.
      Cottam, D. W. and Rees, R. C. 1993. Intl J. Oncol. 2, 861.
      Fujimoto, N., et al. 1993. Clinica Chimica Acta. 221, 91.
      Nakajima, M., et al. 1993. Cancer Res. 53, 5802.
      Stetler-Stevenson, W. G., et al. 1993. FASEB J. 7, 1434.
      Zhang, J., et al. 1993. Clinica Chimica Acta. 219, 1.
      Zucker, S., et al. 1993. Cancer Res. 53, 140.
      Obata, K., et al. 1992. Clinica Chimica Acta. 211, 59.
      Woessner, J. F. 1991. FASEB J. 5, 2145.
      Liotta, L. A. and Stetler-Stevenson, W. G. 1990. in Seminars in Cancer Biology, ed. M. M. Gottesman. Vol.1(2); 99-102.
      Kodama, S., et al. 1987. Collagen Rel. Res. 7, 341.
      Application referencesMMP-9 (Ab-2) Frozen and Paraffin Sections Aikawa, M, et al. 1998. Circ. Res. 83, 1015. MMP-2 (Ab-3) Paraffin Sections: Tsuchiya, Y., et al. 1994. Int. J. Cancer. 56, 46. MMP-1 (Ab-1), MMP-2 (Ab-3), and MMP-3 (Ab-1) Frozen Sections Nakagawa, T., et al. 1994. J. Neurosurg. 81, 69. MMP-1 (Ab-1) Paraffin Sections: Zhang, J., et al. 1993. Clinica Chimica Acta. 219, 1. MMP-2 (Ab-3) Paraffin Sections Nomura H., et al. 1996. Int. J. Cancer 69, 9. Obata, K., et al. 1992. Clinica Chimica Acta. 211, 59. (called clone B) MMP-9 (Ab-1) Enzyme Inhibition: Ramos-DeSimone, N., et al. 1993. Hybridoma 12, 349. MMP-9 (Ab-1) and MMP-9 (Ab-2) Immunoprecipitation Ramos-DeSimone, N. and French, D. L. 1994. Ann. NY Acad. Sci. 732, 469. TIMP-1 (Ab-1) Imunohistochemistry Okada, Y., et al. 1990. Virchows Arch. B Cell Pathol. 59, 305. TIMP-2 (Ab-1) Immunoprecipitation and Frozen Sections Höyhtyä, M., et al. 1994. Int. J. Cancer. 56, 500.