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48-690MAG MILLIPLEX MAP T-Cell Receptor Signaling Magnetic Bead Kit 7-Plex - Cell Signaling Multiplex Assay

48-690MAG
96-well plate  
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      Overview

      Replacement Information

      Key Specifications Table

      Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
      CD3ε (pan Tyr) CREB (S133) ERK (Thr185/Tyr187) LAT (pan Tyr) Lck (pan Tyr) Syk (pan Tyr) ZAP-70 (pan Tyr) HMplexLuminex xMAP
      Description
      Catalogue Number48-690MAG
      Trade Name
      • MILLIPLEX
      DescriptionMILLIPLEX MAP T-Cell Receptor Signaling Magnetic Bead Kit 7-Plex - Cell Signaling Multiplex Assay
      OverviewThe MILLIPLEX® MAP 7-Plex Human T-Cell Receptor (TCR) Magnetic Bead Kit is used to detect changes in phosphorylated CREB (Ser133), Erk/MAP kinase 1/2 (Thr185/Tyr187), and phosphorylated tyrosine residues on CD3 epsilon chain, Lck, ZAP-70, LAT, and Syk in T-cell lysates using the Luminex® system. Although the protein kinase Syk is not part of the T-Cell Receptor pathway, it does play a significant role in B-Cell Receptor signaling and acts as a negative control for the analysis of T-Cell Receptor signaling. The detection assay is a rapid, convenient alternative to Western Blotting and immunoprecipitation procedures.
      References
      Product Information
      Detection methodLuminex xMAP
      ConfigurationPremixed
      Precision, %
      • Intra-Assay: 3% - 8%
      • Inter-Assay: 4% - 17%
      Panel TypeCell Signaling
      Quality LevelMQ200
      Applications
      ApplicationThis cell signaling panel includes the following analytes in this multiplex kit: CD3ε (pan Tyr), CREB (S133), ERK (Thr185/Tyr187), LAT (pan Tyr), Lck (pan Tyr), Syk (pan Tyr), ZAP-70 (pan Tyr).
      Key Applications
      • Multiplexing
      Application Notes• An overnight (4°C) incubation is recommended for best results.
      • This assay requires 25 µL diluted cell lysate.
      • This kit must be run using Assay Buffer 2 (provided).
      • 1 - 25 μg cell lysate/well (recommended starting concentration is 20 μg cell lysate/well).
      Biological Information
      SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
      Species Reactivity
      • Human
      Analytes Available
      • CD3ε (pan Tyr)
      • CREB (S133)
      • ERK (Thr185/Tyr187)
      • LAT (pan Tyr)
      • Lck (pan Tyr)
      • Syk (pan Tyr)
      • ZAP-70 (pan Tyr)
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage for kit components is 2 - 8°C
      Packaging Information
      Material Size96-well plate
      Transport Information
      Supplemental Information
      Specifications