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48-611MAG MILLIPLEX Akt/mTOR Phosphoprotein Magnetic Bead 11-Plex Kit - Cell Signaling Multiplex Assay

48-611MAG
96-well plate  96-well plate
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      Overview

      Replacement Information

      Key Specifications Table

      Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
      Akt (Ser473) GSK3α (Ser21) GSK3β (Ser9) IGF1R (Tyr1135/Tyr1136) IR (Tyr1162/Tyr1163) IRS1 (Ser636) mTOR (Ser2448) p70S6K (Thr412) PTEN (Ser380) RPS6 (Ser235/Ser236) TSC2 (Ser939) HMplexLuminex xMAP
      Description
      Catalogue Number48-611MAG
      Trade Name
      • MILLIPLEX
      DescriptionMILLIPLEX Akt/mTOR Phosphoprotein Magnetic Bead 11-Plex Kit - Cell Signaling Multiplex Assay
      OverviewThe Akt signaling pathway, one of the most often dysregulated signaling pathways in cancer, plays an important role in mediating a very broad range of cellular processes such as growth and development, cell cycle, energy homeostasis, and cell survival. Akt, a serine/threonine kinase that phosphorylates over 100 protein substrates, mediating cell survival and proliferation signals, is often itself hyperphosphorylated in various tumor types. Although the Akt gene itself is not known to be mutated in cancers, many of the upstream regulators of Akt, including IR, IRS, PI3K and PTEN, are oncogenes and tumor suppressors. Downstream of Akt, the mammalian Target Of Rapamycin (mTOR) complex is a key regulator of growth and metabolism. As nearly all of the players in the Akt/mTOR signaling pathway are coordinately regulated by phosphorylation, understanding the role of this pathway in normal physiological processes and in diseases such as cancer and diabetes requires the ability to simultaneously measure phosphorylation status of multiple protein targets.
      References
      Product Information
      Detection methodLuminex xMAP
      ConfigurationPremixed
      Precision, %
      • Intra-Assay: < 7
      • Inter-Assay: < 20
      Panel TypeCell Signaling
      Quality LevelMQ200
      Applications
      ApplicationIntracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation and total pathway proteins in tissue and cell lysate samples. Compare Multiplexing results to those of Western blotting.
      Key Applications
      • Multiplexing
      Application Notes• An overnight (4°C) incubation is recommended for best results.
      • This assay requires 25 µL diluted cell lysate per well.
      • This kit must be run using Assay Buffer 2 (provided).
      • The suggested working range of protein concentration for the assay is 15 to 150 ng of total protein/well (25 μL/well at 600 to 6,000 ng/mL).
      Biological Information
      SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
      Species Reactivity
      • Human
      Analytes Available
      • Akt (Ser473)
      • GSK3α (Ser21)
      • GSK3β (Ser9)
      • IGF1R (Tyr1135/Tyr1136)
      • IR (Tyr1162/Tyr1163)
      • IRS1 (Ser636)
      • mTOR (Ser2448)
      • p70S6K (Thr412)
      • PTEN (Ser380)
      • RPS6 (Ser235/Ser236)
      • TSC2 (Ser939)
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage for kit components is 2 - 8°C.
      Packaging Information
      Material Size96-well plate
      Material Package96-well plate
      Transport Information
      Supplemental Information
      Specifications