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440319 Lumican, His•Tag®, CF, Mouse, Recombinant

440319
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Overview

Replacement Information

Key Specifications Table

Purity
≥95% by SDS-PAGE

Pricing & Availability

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440319-50UG
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      Description
      OverviewRecombinant, murine lumican, consisting of amino acids 19-338, fused at the C-terminus to a His•Tag® sequence, and expressed in NS0-derived mouse myeloma cells. Lumincan a 40-kDa member of the small leucine-rich repeat proteoglycans (SLRPs) family/Class II subfamily of proteins. It is known for its ability to modulate collagen fibrillogenesis and role in cell proliferation regulation.
      Catalogue Number440319
      Brand Family Calbiochem®
      References
      ReferencesD'Onofrio, M.F. et al. 2008. Biochem. Biophys. Res. Commun. 365, 266.
      Nikitovic, D. et al. 2008. IUBMB Life 60, 818.
      Nikitovic, D. et al. 2008. FEBS J. 275, 350.
      Ishiwata, T. et al. 2007. Oncol. Rep. 18, 537.
      Vuillermoz, B. et al. 2004. Exp. Cell Res. 296,294.
      Chakravarti, S. et al. 2003. Invest. Ophthalmol. Vis. Sci. 44, 2422.
      Jepsen, K.J. et al. 2002. J. Biol. Chem. 277, 35532.
      Chakravarti, S. et al. 2000. Invest. Ophthalmol. Vis. Sci. 41,3365.
      Blochberger, T.C. et al. 1992. J. Biol. Chem. 267, 347.
      Chakravarti, S. et al. 1998. J. Cell Biol. 141, 1277.
      Product Information
      FormLyophilized
      FormulationLyophilized from 0.2 µm filtered PBS.
      Quality LevelMQ100
      Applications
      Biological Information
      Biological activityCell adhesion: 50-70% after 50 min at 37°C as measured by the ability of immobilized lumican to supoprt the adhesion of 5 X 10⁴ BCE C/D-1b bovine cornea-derived endothelial cells.
      Purity≥95% by SDS-PAGE
      Physicochemical Information
      ContaminantsEndotoxin: ≤1.0 EU/µg protein
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Lumican, His•Tag®, CF, Mouse, Recombinant SDS

      Title

      Safety Data Sheet (SDS) 

      Lumican, His•Tag®, CF, Mouse, Recombinant Certificates of Analysis

      TitleLot Number
      440319

      References

      Reference overview
      D'Onofrio, M.F. et al. 2008. Biochem. Biophys. Res. Commun. 365, 266.
      Nikitovic, D. et al. 2008. IUBMB Life 60, 818.
      Nikitovic, D. et al. 2008. FEBS J. 275, 350.
      Ishiwata, T. et al. 2007. Oncol. Rep. 18, 537.
      Vuillermoz, B. et al. 2004. Exp. Cell Res. 296,294.
      Chakravarti, S. et al. 2003. Invest. Ophthalmol. Vis. Sci. 44, 2422.
      Jepsen, K.J. et al. 2002. J. Biol. Chem. 277, 35532.
      Chakravarti, S. et al. 2000. Invest. Ophthalmol. Vis. Sci. 41,3365.
      Blochberger, T.C. et al. 1992. J. Biol. Chem. 267, 347.
      Chakravarti, S. et al. 1998. J. Cell Biol. 141, 1277.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision22-June-2011 RFH
      DescriptionRecombinant, murine lumican, consisting of amino acids 19-338, fused at the C-terminus to a His•Tag® sequence, and expressed in NS0-derived mouse myeloma cells. Lumincan a 40-kDa member of the small leucine-rich repeat proteoglycans (SLRPs) family/Class II subfamily of proteins. It is known for its ability to modulate collagen fibrillogenesis and role in cell proliferation regulation.
      FormLyophilized
      FormulationLyophilized from 0.2 µm filtered PBS.
      Purity≥95% by SDS-PAGE
      ContaminantsEndotoxin: ≤1.0 EU/µg protein
      Biological activityCell adhesion: 50-70% after 50 min at 37°C as measured by the ability of immobilized lumican to supoprt the adhesion of 5 X 10⁴ BCE C/D-1b bovine cornea-derived endothelial cells.
      SolubilityReconstitute to 200 µg/ml with sterile PBS.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
      Toxicity Standard Handling
      ReferencesD'Onofrio, M.F. et al. 2008. Biochem. Biophys. Res. Commun. 365, 266.
      Nikitovic, D. et al. 2008. IUBMB Life 60, 818.
      Nikitovic, D. et al. 2008. FEBS J. 275, 350.
      Ishiwata, T. et al. 2007. Oncol. Rep. 18, 537.
      Vuillermoz, B. et al. 2004. Exp. Cell Res. 296,294.
      Chakravarti, S. et al. 2003. Invest. Ophthalmol. Vis. Sci. 44, 2422.
      Jepsen, K.J. et al. 2002. J. Biol. Chem. 277, 35532.
      Chakravarti, S. et al. 2000. Invest. Ophthalmol. Vis. Sci. 41,3365.
      Blochberger, T.C. et al. 1992. J. Biol. Chem. 267, 347.
      Chakravarti, S. et al. 1998. J. Cell Biol. 141, 1277.