The increasing sensitivity of analytical techniques such as UHPLC, LC-MS or LC-MS/MS requires scientists to use specifically adapted reagents. The LC-Pak® Polisher answers this need by further purifying ultrapure water to make it suitable for critical organic molecule analysis at trace and ultra-trace levels.
The LC-Pak® is designed to be connected to the outlet of ultrapure water equipment, in particular, the Q-POD® dispenser of Milli-Q® systems and is warranted to deliver at least 500 liters. To ensure ultra-trace organic levels are reached, the LC-Pak® technology is based on the proven C18 reverse-phase silica purification media.
The granular C18 reverse-phase silica in the polypropylene housing is tightly packed by a compression disc to avoid any channeling effect. This ensures that the water passing through the LC-Pak® is in close contact with the C18 chains binding organic molecules by hydrophobic interactions.
As a result, the now improved water quality is perfectly suitable for the production of mobile phase, buffers, blanks, standards preparation, sample dilution, glassware rinsing or extraction used in these critical applications. Each LC-Pak® Polisher is delivered with its Certificate of Quality.
These results were obtained using an LC-Pak® connected at the outlet of a Milli-Q® ultrapure (Type 1) system with TOC < 5 ppb.
|Parameter||Specification of LC-Pak® Polisher ultrapure water||Comments|
|HPLC Gradient Test - Absorbance of highest eluted peak||At 210 nm <0.006 AU
At 254 nm <0.002 AU
|Concentration of 60 mL water at 1 mL/min prior to elution|
|HPLC Gradient Test - Absorbance of highest eluted peak||At 210 nm <0.003 AU
At 254 nm <0.001 AU
|No water pre-concentration|
|Optical properties: absorbance in UV range||UV 200nm <0.05 AU
UV 205nm <0.01 AU
UV 210nm <0.01 AU
UV 254nm <0.005 AU
|Fluorescence as quinine||At 254nm <1 ppb
At 365nm <1 ppb
|Compliance with suitability for LC/MS: Reserpine test||No peak higher than 10 ppb Reserpine at 609 m/z in ESI +|
|Residue after evaporation||<0.0001% w/w||Test performed as specified in ISO 3696 procedure|