71087 KOD XL DNA Polymerase

71087
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          Description
          OverviewPCR involves replication of a DNA template by a thermostable DNA polymerase. The processivity, specificity, and fidelity of the polymerase enzyme used can influence the efficiency, reproducibility, and yield of the PCR reaction. High-fidelity PCR, utilizes a DNA polymerase with a low error rate and results in a high degree of accuracy in the replication of the DNA of interest. Fidelity is critical when accurate sequence amplification of the gene target is needed, for example, when direct sequencing or cloning for downstream protein expression. Unwarranted mutation could severely impact your studies. Our analysis has shown that KOD enzymes are an easy choice for fast, accurate and high-yielding PCR. EMD Millipore's molecular biologists work to develop and formulate polymerases offering the highest specificity, fidelity and yield during PCR amplification. In addition, optimized buffer compositions, convenient master mixes and cycling parameters provide additional ease of use and data reproducibility.
          KOD XL DNA Polymerase* is an optimized blend of KOD DNA Polymerase and a mutant form of KOD that is deficient in 3′→5′ exonuclease activity (Nishioka 2002). This enzyme mixture is designed for reliable amplification of long, complex targets with robust yield and high accuracy. It can also be used for incorporation of derivatized dNTPs in PCR amplicons (Sawai 2002, Sawai 2001). KOD XL DNA Polymerase generates a mixture of PCR products with blunt and 3′-dA overhangs, suitable for cloning with Novagen Perfectly Blunt®, AccepTor™, and LIC Vector Kits.


          Source Recombinant Thermococcus kodakaraensis KOD1 DNA polymerase expressed in E. coli (wild type and exonuclease-deficient forms)
          Concentration 2.5 U/µl
          Endonuclease None detected
          Nicking activity None detected
          Amplification effiency Functional PCR
          Storage –20°C

          *Manufactured by Toyobo and distributed by Novagen. Not available from Novagen in Japan. Licensed under US Patent Number 5,436,149 owned by Takara Shuzo Co., Ltd.

          Note: Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patents rights (such as 5' Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA.

          Catalogue Number71087 Brand Family Novagen®
          Features and benefits

          • Ideal for amplification of large DNA fragments from purified DNA or crude samples
          • Amplifies DNA templates up to 30 kbp
          • Successfully amplifies GC-rich sequences
          • Efficiently incorporates derivatized dNTPs
          References
          ReferencesNishioka, M., et al. 2002. J. Biotechnol. 88, 141. Sawai, H., et al. 2002. Bioconjugate Chem. 13, 309. Sawai, H., et al. 2001. Chem. Commun. 24, 2604.
          Product Information
          Unit of DefinitionOne unit is defined as the amount of enzyme that will catalyze the incorporation of 10 nmol of dNTP into acid-insoluble form in 30 min at 75°C, in a reaction containing 20 mM Tris-HCl (pH 7.5 at 25°C), 8 mM MgCl₂, 7.5 mM DTT, 50 µg/ml BSA, 150 µM each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [<Sup>3</Sup>H]dTTP), and 150 µg/ml activated calf thymus DNA.
          Components
          DeclarationManufactured by Toyobo and distributed by Novagen. Not available from Novagen in Japan. Purchase of this product is accompanied by a limited license to use it in the Polymerase Chain Reaction (PCR) process for research use in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by the up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., an authorized thermal cycler.
          Quality LevelMQ100
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Shipped with Blue Ice or with Dry Ice
          Toxicity Standard Handling
          Storage -20°C
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          KOD XL DNA Polymerase Certificates of Analysis

          TitleLot Number
          71087

          References

          Reference overview
          Nishioka, M., et al. 2002. J. Biotechnol. 88, 141. Sawai, H., et al. 2002. Bioconjugate Chem. 13, 309. Sawai, H., et al. 2001. Chem. Commun. 24, 2604.

          Citations

          Title
        • Gang Wu, et al. (2006) Simplified gene synthesis: A one-step approach to PCR-based gene construction. Journal of Biotechnology 124, 496-503.
        • Tom S. Kim, et al. (2005) Delayed dark adaptation in 11-cis-retinol dehydrogenase deficient mice: a role of RDH11 in visual processes in vivo. Journal of Biological Chemistry 280, 8694-8704.
        • Yukio Kuwada, et al. (2003) Potential involvement of IL-8 and its receptors in the invasiveness of pancreatic cancer cells. International Journal of Oncology 22, 765-771.
        • Yoko Ogawara, et al. (2002) Akt enhances Mdm2-mediated ubiquitination and degradation of p53. Journal of Biological Chemistry 277, 21843-21850.
        • Hiroshi Oyama, et al. (2002) A CLN2-related and thermostable serine-carboxyl proteinase, kumamolysin: cloning, expression, and identification of catalytic serine residue. 131, [757-765.
        • Ichiro Tabuchi, et al. (2002) An efficient ligation method in the making of an in vitro virus for in vitro protein evolution. Biological Procedures Online 4, 49-54.
        • Toshiyuki Sasagawa, et al. (2001) High-risk and multiple human papillomavirus infections associated with cervical abnormalities in Japanese women. Cancer Epidemiology Biomarkers and Prevention 10, 45-52.
        • Toru Tsuji, Michiko Onimaru and Hiroshi Yanagawa. (2001) Random multi-recombinant PCR for the construction of combinatorial protein libraries. Nucleic Acids Research 29, e97.
        • Kunitoshi Mitsumori, et al. (2000) Rapid induction of uterine tumors with p53 point mutations in heterozygous p53-deficient CBA mice given a single intraperitoneal administration of N-ethyl-N-nitrosourea. Carcinogenesis 21, 1039-1042.
        • Akihiro Saito, et al. (2000) Transcriptional co-regulation of five chitinase genes scattered on the Streptomyces coelicolor A3(2) chromosome. Microbiology 146, 2937-2946.
        • Tsutomu Tanaka, et al. (2000) A novel form of prostate-specific antigen transcript produced by alternative splicing. Cancer Research 60, 56-59.
        • Xiao-Feng Tang, et al. (2000) Biochemical analysis of a thermostable tryptophan synthase from a hyperthermophilic archaeon. European Journal of Biochemistry 267, 6369-6377.
        • Hitoshi Nakayashikia, et al. (1999) Transposition of the retrotransposon MAGGY in heterologous species of filamentous fungi. Genetics 153, 693-703.
        • User Protocols

          Title
          TB342 KOD XL DNA Polymerase

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