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407252 Insulin-Like Growth Factor-I, Mouse, Recombinant, E. coli

407252
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Overview

Replacement Information

Key Specifications Table

Purity
≥98% by SDS and HPLC

Pricing & Availability

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407252-100UG
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      Plastic ampoule 100 μg
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      Description
      OverviewFull-length, recombinant, mouse insulin-like growth factor I (IGF-I) expressed in E. coli. IGF-I regulates proliferation and survival signals through the Akt pathway. Mouse IGF-I shares 94% sequence identity with human IGF-I and exhibits cross-species reactivity. Useful in the study of cell proliferation and survival signals.
      Catalogue Number407252
      Brand Family Calbiochem®
      SynonymsmIGF-I
      References
      ReferencesClemmons, D.R., et al. 2006. Curr. Opin. in Pharmacol. 6, 620.
      Bell, G.I., et al. 1986. Nucleic Acids Res. 14, 7873.
      Product Information
      FormLyophilized
      FormulationSterile-filtered prior to lyophilization. Contains no additives.
      Quality LevelMQ100
      Applications
      Biological Information
      Biological activityED₅₀: ≤2 ng/ml (corresponding to a specific activity of ≥1 x 10⁶ units/mg) as determined by a cell proliferation assay using FDC-P1 cells. For most in vitro applications IGF-I exerts is biological activity in the concentration range of 1-20 ng/ml.
      Purity≥98% by SDS and HPLC
      Physicochemical Information
      ContaminantsEndotoxin: ≤1 ng/µg IGF-I
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C. Avoid freeze/thaw cycles of solutions. Aliquots may be stored at 4°C for up to 1 week without loss of biological activity.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Insulin-Like Growth Factor-I, Mouse, Recombinant, E. coli SDS

      Title

      Safety Data Sheet (SDS) 

      Insulin-Like Growth Factor-I, Mouse, Recombinant, E. coli Certificates of Analysis

      TitleLot Number
      407252

      References

      Reference overview
      Clemmons, D.R., et al. 2006. Curr. Opin. in Pharmacol. 6, 620.
      Bell, G.I., et al. 1986. Nucleic Acids Res. 14, 7873.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision14-October-2008 JSW
      SynonymsmIGF-I
      DescriptionFull-length, recombinant, mouse insulin-like growth factor I (IGF-I) expressed in E. coli. IGF-I regulates proliferation and survival signals through the Akt pathway. Mouse IGF-I shares 94% sequence identity with human IGF-I and exhibits cross-species reactivity. Useful in the study of cell proliferation and survival signals.
      FormLyophilized
      FormulationSterile-filtered prior to lyophilization. Contains no additives.
      Purity≥98% by SDS and HPLC
      ContaminantsEndotoxin: ≤1 ng/µg IGF-I
      Biological activityED₅₀: ≤2 ng/ml (corresponding to a specific activity of ≥1 x 10⁶ units/mg) as determined by a cell proliferation assay using FDC-P1 cells. For most in vitro applications IGF-I exerts is biological activity in the concentration range of 1-20 ng/ml.
      SolubilityReconstitute with sterile H₂O to a final concentration of 0.1-1 mg/ml. Further dilute with aqueous buffer before use.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C. Avoid freeze/thaw cycles of solutions. Aliquots may be stored at 4°C for up to 1 week without loss of biological activity.
      Toxicity Standard Handling
      ReferencesClemmons, D.R., et al. 2006. Curr. Opin. in Pharmacol. 6, 620.
      Bell, G.I., et al. 1986. Nucleic Acids Res. 14, 7873.