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400053 Hygromycin B, Streptomyces sp., Sterile-Filtered Solution in 25 mM HEPES, Cell Culture-Tested - CAS 31282-04-9 - Calbiochem

400053
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Overview

Replacement Information

Key Specifications Table

CAS #Empirical Formula
31282-04-9C₂₀H₃₇N₃O₁₃

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400053-20ML
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      400053-5ML
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          Description
          OverviewSterile liquid formulation of Cat. No. 400050.
          Catalogue Number400053
          Brand Family Calbiochem®
          References
          ReferencesSugimoto, K., et al. 1994. Plant J. 5, 863.
          Lee, M.G., and van der Ploeg, L.H. 1991. Gene 105, 255.
          Salauze, D., et al. 1990. Antimicrob. Agents Chemother. 34, 1915.
          Cullen, D., et al. 1987. Gene 57, 21.
          Santerre, R.F., et al. 1984. Gene 30, 147.
          Rao, S.N., et al. 1983. Antimicrob. Agents Chemother. 24, 689.
          Gonzalez, A., et al. 1978. Biochim. Biophys. Acta 521, 459.
          Product Information
          CAS number31282-04-9
          FormLiquid
          Formulation50 mg/ml active antibiotic in sterile-filtered 25 mM HEPES, pH 7.2.
          Hill FormulaC₂₀H₃₇N₃O₁₃
          Chemical formulaC₂₀H₃₇N₃O₁₃
          Structure formula ImageStructure formula Image
          Quality LevelMQ100
          Applications
          ApplicationHygromycin B is an aminoglycoside antibiotic that inhibits the growth of prokaryotic and eukaryotic microorganisms and mammalian cells. Inhibits protein synthesis by interfering with the translocation of the 70S ribosome and causes misreading of mRNA.
          Biological Information
          Purity≥90% by HPLC
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          RTECSWK2130000
          Safety Information
          R PhraseR: 26/27/28-37/38-41

          Very toxic by inhalation, in contact with skin and if swallowed.
          Irritating to respiratory system and skin.
          Risk of serious damage to eyes.
          S PhraseS: 23-26-36/37/39-45

          Do not breathe fumes.
          In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
          Wear suitable protective clothing, gloves and eye/face protection.
          In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Highly Toxic & Carcinogenic / Teratogenic
          Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Hygromycin B, Streptomyces sp., Sterile-Filtered Solution in 25 mM HEPES, Cell Culture-Tested - CAS 31282-04-9 - Calbiochem SDS

          Title

          Safety Data Sheet (SDS) 

          Hygromycin B, Streptomyces sp., Sterile-Filtered Solution in 25 mM HEPES, Cell Culture-Tested - CAS 31282-04-9 - Calbiochem Certificates of Analysis

          TitleLot Number
          400053

          References

          Reference overview
          Sugimoto, K., et al. 1994. Plant J. 5, 863.
          Lee, M.G., and van der Ploeg, L.H. 1991. Gene 105, 255.
          Salauze, D., et al. 1990. Antimicrob. Agents Chemother. 34, 1915.
          Cullen, D., et al. 1987. Gene 57, 21.
          Santerre, R.F., et al. 1984. Gene 30, 147.
          Rao, S.N., et al. 1983. Antimicrob. Agents Chemother. 24, 689.
          Gonzalez, A., et al. 1978. Biochim. Biophys. Acta 521, 459.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision06-September-2007 JSW
          DescriptionHygromycin B is an aminoglycoside antibiotic, inhibits the growth of prokaryotic and eukaryotic microorganisms and mammalian cells. Inhibits protein synthesis by interfering with the translocation of the 70S ribosome and causes misreading of mRNA. Resistance to hygromycin B is conferred by a gene whose product, hygromycin B phospho-transferase that phosphorylates and inactivates the drug. Hygromycin B has been used to select mutants carrying the hygromycin B resistance gene in a wide variety of cells including bacterial, protozoal, yeast, fungal, plant, and mammalian cells. Typical concentrations used for mammalian cell selection are between 0.05 to 1 mg/ml. The appropriate concentration for use must be determined empirically for each cell type.
          FormLiquid
          Formulation50 mg/ml active antibiotic in sterile-filtered 25 mM HEPES, pH 7.2.
          CAS number31282-04-9
          RTECSWK2130000
          Chemical formulaC₂₀H₃₇N₃O₁₃
          Structure formulaStructure formula
          Purity≥90% by HPLC
          Storage +2°C to +8°C
          Do Not Freeze Ok to freeze
          Toxicity Highly Toxic & Carcinogenic / Teratogenic
          Merck USA index14, 4852
          ReferencesSugimoto, K., et al. 1994. Plant J. 5, 863.
          Lee, M.G., and van der Ploeg, L.H. 1991. Gene 105, 255.
          Salauze, D., et al. 1990. Antimicrob. Agents Chemother. 34, 1915.
          Cullen, D., et al. 1987. Gene 57, 21.
          Santerre, R.F., et al. 1984. Gene 30, 147.
          Rao, S.N., et al. 1983. Antimicrob. Agents Chemother. 24, 689.
          Gonzalez, A., et al. 1978. Biochim. Biophys. Acta 521, 459.

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          400051 Hygromycin B, Streptomyces sp. - CAS 31282-04-9 - Calbiochem Show Pricing & Availability
          400052 Hygromycin B, Streptomyces sp., Sterile-Filtered Solution in PBS, Cell Culture-Tested - CAS 31282-04-9 - Calbiochem Show Pricing & Availability

          Categories

          Life Science Research > Cell Culture and Systems > Antibiotics & Selection Agents
          Life Science Research > Inhibitors and Biochemicals > Biochemicals > Antibiotics > Antimicrobial Agents