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374805 Hemocyanin, Keyhole Limpet, Megathura crenulata

374805
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Overview

Replacement Information

Key Specifications Table

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374805-1GM
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      Plastic ampoule 1 gm
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      374805-250MG
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          Description
          OverviewStable solid containing more than 60% protein. Protein is 90% undissociated hemocyanin.
          Protein:≥60%; copper to protein ratio: ≥1.5 x 10-3
          Catalogue Number374805
          Brand Family Calbiochem®
          SynonymsKLH
          References
          ReferencesHayashi, K., et al. 1992. Virchows Archiv. B Cell Pathol. 63, 37.
          Shuler, K.R., et al. 1992. J. Immunol. Methods 156, 137.
          Erlanger, B.F. 1980. Methods Enzymol. 70, 85.
          Product Information
          CAS number9013-72-3
          FormBlue to blue-gray lyophilized solid
          FormulationLyophilized from BES buffer, MgSO₄.
          PreservativeNone
          Quality LevelMQ200
          Applications
          ApplicationHemocyanin, Keyhole Limpet, Megathura crenulata, CAS 9013-72-3, is a large, multi-subunit, oxygen-carrying, metalloprotein that is used as a carrier protein in the production of antibodies.
          Application CommentsProtein concentration: ≥60%
          Copper: ≥0.09%
          Copper to Protein ratio: ≥1.5 x 10-3
          Biological Information
          Purity≥90% by size exclusion chromatography
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
          Special InstructionsDissolve at 5-10 mg/ml (protein) in PBS or other physiological buffer by GENTLE addition of the buffer (e.g. slowly added down the side of the tube). DO NOT vortex or agitate vigorously to dissolve product. (KLH is an extremely large protein complex and has a tendency to aggregate and precipitate from solutions.) For further dilutions, use an appropriate buffer and maintain the magnesium concentration at ≥10 mM. Solutions should be stored in the refrigerator (4°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Hemocyanin, Keyhole Limpet, Megathura crenulata SDS

          Title

          Safety Data Sheet (SDS) 

          Hemocyanin, Keyhole Limpet, Megathura crenulata Certificates of Analysis

          TitleLot Number
          374805

          References

          Reference overview
          Hayashi, K., et al. 1992. Virchows Archiv. B Cell Pathol. 63, 37.
          Shuler, K.R., et al. 1992. J. Immunol. Methods 156, 137.
          Erlanger, B.F. 1980. Methods Enzymol. 70, 85.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision10-March-2011 JSW
          SynonymsKLH
          DescriptionHemocyanin purified from Keyhole Limpet (Mega thura crenulata). Protein:≥60%; copper to protein ratio: ≥1.5 x 10-3
          BackgroundKeyhole limpet hemocyanin (KLH), a carrier protein with a molecular weight ranging from 3,000,000-7,500,000, has many available primary amines to facilitate protein conjugation. KLH is used to immunize animals to elicit antibodies to small molecules (haptens) by covalent conjugation.
          FormBlue to blue-gray lyophilized solid
          FormulationLyophilized from BES buffer, MgSO₄.
          CAS number9013-72-3
          Purity≥90% by size exclusion chromatography
          PreservativeNone
          CommentsProtein concentration: ≥60%
          Copper: ≥0.09%
          Copper to Protein ratio: ≥1.5 x 10-3
          Storage +2°C to +8°C
          Do Not Freeze Ok to freeze
          Special InstructionsDissolve at 5-10 mg/ml (protein) in PBS or other physiological buffer by GENTLE addition of the buffer (e.g. slowly added down the side of the tube). DO NOT vortex or agitate vigorously to dissolve product. (KLH is an extremely large protein complex and has a tendency to aggregate and precipitate from solutions.) For further dilutions, use an appropriate buffer and maintain the magnesium concentration at ≥10 mM. Solutions should be stored in the refrigerator (4°C).
          Toxicity Standard Handling
          Merck USA index14, 4647
          ReferencesHayashi, K., et al. 1992. Virchows Archiv. B Cell Pathol. 63, 37.
          Shuler, K.R., et al. 1992. J. Immunol. Methods 156, 137.
          Erlanger, B.F. 1980. Methods Enzymol. 70, 85.

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          Categories

          Life Science Research > Protein Detection and Quantification > Immunodetection Support Products > Adjuvants & Immunization Tools