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368067 Granzyme B Substrate VIII, Colorimetric - Calbiochem

368067
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368067-5MG
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      Description
      OverviewA colorimetric substrate for granzyme B (kcat/Km = 6.6 x 104 M-1s-1). Cleavage of pNA is monitored colorimetrically at ~405 nm (ε = 9,160 M-1cm-1).
      Catalogue Number368067
      Brand Family Calbiochem®
      SynonymsAc-IEPD-pNA
      References
      ReferencesHarris, J.L., et al. 1998. J. Biol. Chem. 273, 27364.
      Thornberry, N.A., et al. 1997. J. Biol. Chem. 272, 17907.
      Product Information
      ATP CompetitiveN
      FormWhite lyophilized solid
      Hill FormulaC₂₈H₃₈N₆O₁₁
      Chemical formulaC₂₈H₃₈N₆O₁₁
      ReversibleN
      Quality LevelMQ100
      Applications
      Biological Information
      Primary TargetColorimetric substrate for granzyme B
      Primary Target IC<sub>50</sub>kcat/Km = 6.6 x 104 M⁻¹ sec⁻¹ as substrate for granzyme B
      Purity≥95% by HPLC
      Physicochemical Information
      Cell permeableN
      Peptide SequenceAc-Ile-Glu-Pro-Asp-pNA
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Granzyme B Substrate VIII, Colorimetric - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      Granzyme B Substrate VIII, Colorimetric - Calbiochem Certificates of Analysis

      TitleLot Number
      368067

      References

      Reference overview
      Harris, J.L., et al. 1998. J. Biol. Chem. 273, 27364.
      Thornberry, N.A., et al. 1997. J. Biol. Chem. 272, 17907.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision07-July-2008 RFH
      SynonymsAc-IEPD-pNA
      DescriptionA colorimetric substrate for granzyme B (kcat/Km = 66,600 M-1s-1). Cleavage of pNA is monitored as an increase in absorbance at ~405 nm (ε = 9,160 M-1cm-1).
      FormWhite lyophilized solid
      Intert gas (Yes/No) Packaged under inert gas
      Recommended reaction conditions0.25 µM substrate, 100 mM HEPES buffer, pH 7.5, and 10 mM DTT. Note: optimal enzyme concentration and incubation time should be determined empirically for each assay.
      Chemical formulaC₂₈H₃₈N₆O₁₁
      Peptide SequenceAc-Ile-Glu-Pro-Asp-pNA
      Purity≥95% by HPLC
      SolubilityDMSO (5 mg/ml)
      Storage -20°C
      Protect from light
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
      Toxicity Standard Handling
      ReferencesHarris, J.L., et al. 1998. J. Biol. Chem. 273, 27364.
      Thornberry, N.A., et al. 1997. J. Biol. Chem. 272, 17907.