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Fuchsin (C.I. 42510)
This staining dye “Fuchsin (C.I. 42510) - for microscopy Certistain®” is used for staining of acid-fast bacteria (AFB) according to Kinyoun and serves the purpose of the bacteriological and histological investigation of sample material of human origin, for example smears of enriched bacterial cultures or histological sections of e. g. the lung. The cell wall of acid-fast bacteria has a high proportion of wax and lipids and hence absorbs dyes only very slowly. As a measure to enhance the absorption of the fuchsin dye and thus the formation of the mycolate-fuchsin complex in the cell wall, the carbolfuchsin solution applied to the specimen is normally heated to evaporation point. If the Kinyoun carbolfuchsin solution is used, the heating of the solution is dispensable. Consequently, the release of hazardous phenolic vapors will be avoided. Once the acid-fast bacteria have absorbed the fuchsin dye, it is virtually impossible to decolorize them again, even when they are intensively treated with a decolorizing solution such as e. g. hydrochloric acid in ethanol. Accordingly, acid-fast bacteria are termed as acid- and alcohol-fast for staining, and are stained red in the microscopic visualization. Correspondingly, all non-acid-fast microorganisms are counterstained with an appropriate dye. In the present operating instructions is counterstained accordance with methylene blue. Pretreatment of the specimens with Sputofluol® dissolves the bacteria from the surrounding viscid sputum and cell material. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
H315: Causes skin irritation. H319: Causes serious eye irritation. H350: May cause cancer. H410: Very toxic to aquatic life with long lasting effects.
P202: Do not handle until all safety precautions have been read and understood. P264: Wash skin thoroughly after handling. P273: Avoid release to the environment. P302 + P352: IF ON SKIN: Wash with plenty of water. P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P308 + P313: IF exposed or concerned: Get medical advice/ attention.
6.1C Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK 3 highly hazardous to water
9 Carcinogenic compounds and flammable compounds labelled "Highly toxic" or "Toxic": container F. Alkyl sulfates are carcinogenic; Take particular care to avoid inhalation and skin contact. To neutralize alkyl sulfates, add dropwise (from a dropping funnel) to concentrated ice-cool Ammonia solution (Cat.No. 105426) with vigorous stirring. Before placing in container D, check the pH with pH Universal indicator strips (Cat.No. 109535).
Categories of danger
Product Usage Statements
Storage and Shipping Information
Store at +2°C to +30°C.
Declaration (railroad and road) ADR, RID
UN 3077 , 9, III
Declaration (transport by air) IATA-DGR
UN 3077 , 9, III
Declaration (transport by sea) IMDG-Code
UN 3077 , 9, III, Marine Pollutant: P
Dye content (spectrophotometrically)
≥ 80 %
Absorption maximum λ max (ethanol 50 %)
549 - 552 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.003 g/l; ethanol 50 %)