344045 FOCUS-Protease Arrest™ Cocktail - Calbiochem

344045
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344045-1ML
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      Plastic ampoule 1 ml
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      Description
      OverviewA protease cocktail specifically developed for 2D gel sample preparation. Inhibits serine, cysteine, metalloproteinases, and calpain proteases from animal tissues, plants, yeast, and bacteria. FOCUS-Protease Arrest™ Cocktail at 1X concentration in extraction buffer, pH 7.0 - 8.0, inhibits 95-98% of protease activity.
      Catalogue Number344045
      Brand Family Calbiochem®
      References
      Product Information
      ATP CompetitiveN
      FormLiquid
      FormulationSupplied as 100X.
      ReversibleN
      Quality LevelMQ100
      Applications
      Biological Information
      Primary TargetSerine, cysteine, metalloproteinases, and calpain proteases
      Physicochemical Information
      Cell permeableN
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      R PhraseR: 36/37/38

      Irritating to eyes, respiratory system and skin.
      S PhraseS: 26-36

      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Irritant
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing dilution in extraction/lysis buffer or extract, the FOCUS-Protease Arrest™ inhibitors are stable for up to 2 weeks at 4°C or up to 4 months at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      FOCUS-Protease Arrest™ Cocktail - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      FOCUS-Protease Arrest™ Cocktail - Calbiochem Certificates of Analysis

      TitleLot Number
      344045
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision28-September-2007 RFH
      DescriptionA protease inhibitor cocktail specifically developed for the inhibition of protease activity during protein purification and sample processing. Suitable for use with yeast, bacterial, plants, and mammalian tissues. No EDTA is used in this preparation. Compatible with FOCUS-Nuclease™ for removing nucleic acid contamination from samples. FOCUS-Protease-Arrest™ contains agents that inhibit serine, cysteine, metallo-proteases as well as calpains. FOCUS-Protease-Arrest™ contains both irreversible and reversible protease inhibitors. FOCUS-Protease Arrest™, used at 1:50 dilution in extraction buffer (pH 7-8) inhibits over 95-98% of protease activities.
      FormLiquid
      FormulationSupplied as 100X.
      Recommended reaction conditions
      1. Allow solution to warm to room temperature. The solution is in suspension form, vortex before pipetting. 2. Add FOCUS-Protease Arrest™ Cocktail at a volume of 10 µl/ml of extraction/lysis buffer or extract to achieve a 1X final concentration. Note: For greater protease inhibition, the Protease Arrest™ Cocktail may be added to extraction/lysis buffer at a volume of 13 µl/ml (1.3X final concentration).
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing dilution in extraction/lysis buffer or extract, the FOCUS-Protease Arrest™ inhibitors are stable for up to 2 weeks at 4°C or up to 4 months at -20°C.
      Toxicity Irritant