L6145-BC Easycoll, Density 1.124 g/ml

L6145-BC
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L6145
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      500 ml
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      Description
      OverviewThe basis of Easycoll is silica gel particles, coated with PVP (polyvinyl pyrrolidone). Biochrom AG ready-to-use preparations are already adjusted to physiological values of osmolality and pH. Any desired densities from Easycoll (Cat. No. L 6143/5) can conveniently be prepared with PBS (Cat. No. L 1825).

      Specifications:

      Sterility: Pass
      pH: 7.02-7.62
      Osmolality: 0.281-0.310 Osm/kg H20
      Density: 1.123-1.125 g/ml
      Color: Light Yellow
      Appearance: Clear

      Separating solutions:

      Separating solutions are used for the isolation of cells or cellular particles during density gradient centrifugation. We offer two different separating solutions: Biocoll and Easycoll. These two solutions differ in the respectively contained polymer. Biocoll separating solution does contain a polymer with a molecular weight of approx. 400000 Dalton. The basis of Easycoll is silica gel particles, coated with PVP (polyvinyl pyrrolidone). The ready-made solutions are already preset on physiological values of osmolality and ph-value.

      Easycoll

      The basis of Easycoll is silica gel particles, coated with PVP (polyvinyl pyrrolidone). Our ready-to-use preparations are already adjusted to physiological values of osmolality and pH. Any desired densities from Easycoll (Cat. No. L6143/5) can conveniently be prepared with PBS (Cat. No. L1825).

      Production of Easycoll with different densities:

      The ready-to-use separating solution Easycoll is already adjusted to physiological conditions of osmolality and pH value. But you can also adjust a required density yourself by diluting Easycoll correspondingly.

      Vol.%Eascoll(1.124 g/ml) = D(g/ml-1.005)/1.19 x 103

      The table below shows an example for different densities. All amounts are given for a temperature of +20 °C. Only PBS w/o Ca2+, Mg2+ is suitable, since these ions might influence agglomeration and hence separation of cell clusters. Preparation of solutions with other densities can be made by isoosmolar dilution according to the following formula:

      V[%] = (D'-D) x 102/D"-D%

      where
      D' is required final density
      D" is high starting density
      D is density of isoosmotic dilution solution (g/ml)
      V% is volume percent of the starting solution with high density
      For the dilution we recommend the use of PBS without Ca2+, Mg2+, since both ions can influence the agglomeration of cells and consequently cell separation.
      Catalogue NumberL6145-BC
      Brand Family Calbiochem®
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      Quality LevelMQ300
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      Ship Code Blue Ice Only
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
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