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L2113-BC EDTA (Versen), 1 %

L2113-BC
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L2113
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      100 ml
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      Description
      OverviewTrypsin/EDTA solutions (0.05 or 0.02% in PBS, without Ca2+, Mg2+) are used for passaging monolayer cultures. Trypsin affects the cell membrane and the cellular, and also the intercellular mucoproteins. Trypsin can be inhibited with the use of serum.

      Recommendation Instructions:

      Recommendations Trypsin (1:250)/EDTA solutions:
      Trypsin (1:250)/EDTA solutions (0.05/0.02 % in PBS without Ca2+, Mg2+) to passage monolayer cultures:
      1. Wash cell culture (monolayer) using physiological saline (w/o Ca2+, Mg2+).
      2. Apply a small quantity of Trypsin (1:250)/EDTA solution with a pipette to cell culture flask; a thin film is sufficient.
      3. Incubate culture flask at +37°C for approx. 5 min.
      4. Centrifuge cell suspension or transfer directly to a serum containing culture medium.

      With higher Trypsin concentrations, it is recommended to trypsinate at +2-+8°C.
      Specifications:

      Functional Test: Pass
      Activity Test: Pass
      pH: 7.2-7.5
      Osmolality: 0.27-0.30 Osm/kg H20
      Color: Colorless
      Appearance: Clear

      Catalogue NumberL2113-BC
      Brand Family Calbiochem®
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      Product Information
      Quality LevelMQ300
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      Ship Code Blue Ice Only
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
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