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CC120 Caspase 9, recombinant human active

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CC120
25 units  
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      Overview

      Replacement Information

      Key Specifications Table

      Key ApplicationsEntrez Gene NumberSpeciesUni Prot Number
      ACTNM_001229.2 NM_032996.1 Human P55211
      Description
      Catalogue NumberCC120
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionCaspase 9, recombinant human active
      OverviewCaspase-9 is a member of the interleukin-1beta converting enzyme (ICE) family of cysteine proteases. Similar to other caspases, caspase-9 also exists in cells as an inactive proenzyme. During the initiation of apoptosis the proenzyme is processed at aspartate residues to form active caspase-9. As one of the initiator caspases, active caspase-9 functions to trigger the activation of downstream effector caspases and further lead to disassembly of cell structures.

      The partially purified active recombinant human caspase-9 with full-length HIS-6 tag was expressed in E. coli. The expressed caspase-9 spontaneously undergoes autoprocessing to yield the subunits characteristic of the native enzyme. In combination with caspase-9 activity assays, the active recombinant caspase-9 is useful in biological screening of caspase inhibitors. The recombinant enzyme can also be used as a positive control in caspase-9 assays. Caspase 9 is known to have strong reactivity with other sequence peptides, especially DEVD-pNA.
      Alternate Names
      • APAF-3
      • ICE-LAP6
      • Mch-6
      References
      Product Information
      PresentationLyophilized. Reconstitute with 25 μl of PBS + 15% glycerol.
      Quality LevelMQ100
      Applications
      Key Applications
      • Activity Assay
      Application NotesEnzyme is prepared as an activity standard only, any other application not tested. Caspase-9 is subject to auto-activation thus active caspase subunits and partially active intermediates will be seen. Bands of approximately 45kDa (pro), 35kDa (partially active) and 18kDa + 12kDa (large and small subunits) may be seen by western blot depending upon the antibody used. The small subunit is extremely difficult to detect in most westerns.

      Activation: incubate 1-5 μL of stock in PBS pH 7.5 (100mM HEPES can be used as well) plus 10mM ATP and 5mM DTT (added just before use) for 30 minutes at 37°C.
      Biological Information
      Purity10-20% caspase-9 protein by gel analysis. Also contains E. coli proteins. Approximately, 0.8-2 μg total protein / unit of activity.
      SourceHuman
      Specific ActivityOne unit of the recombinant caspase-9 is the enzyme activity that cleaves 1 nmol of the caspase substrate LEHD-pNA (pNA: pnitroanaline) per hour at 37°C at saturated substrate concentrations.
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade. Alternative splicing results in two transcript variants which encode different isoforms.
      Gene Symbol
      • CASP9
      • CASP-9
      • ICE-LAP6
      • MCH6
      • APAF3
      • CASPASE-9c
      • OTTHUMP00000044594
      • APAF-3
      • EC 3.4.22.62 [Contains: Caspase-9 subunit p35
      • Caspase-9 subunit p10].
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P55211 # Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
      SIZE: 416 amino acids; 46281 Da
      SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 35 kDa (p35) and a 10 kDa (p10) subunit. Caspase-9 and APAF1 bind to each other via their respective NH2-terminal CED-3 homologous domains in the presence of cytochrome C and ATP. Interacts with the inhibitors BIRC2, BIRC4, BIRC5 and BIRC7.
      TISSUE SPECIFICITY: Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues.
      PTM: Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase- 3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
      SIMILARITY: SwissProt: P55211 ## Belongs to the peptidase C14 family. & Contains 1 CARD domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -70°C in undiluted aliquots for 3-6 months from date of receipt. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size25 units
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      CC120 08436037125072

      Documentation

      Caspase 9, recombinant human active Certificates of Analysis

      TitleLot Number
      HUMAN CASPASE-9 (ACTIVE) RECOMBINANT PROTEIN 3154981
      HUMAN CASPASE-9 (ACTIVE) RECOMBINANT PROTEIN 2925404
      HUMAN CASPASE-9 (ACTIVE) RECOMBINANT PROTEIN 2482216
      HUMAN CASPASE-9 (ACTIVE) RECOMBINANT PROTEIN - 2494611 2494611
      HUMAN CASPASE-9 (ACTIVE) RECOMBINANT PROTEIN - 1990903 1990903
      HUMAN CASPASE-9 (ACTIVE) RECOMBINANT PROTEIN - 2074750 2074750
      HUMAN CASPASE-9 (ACTIVE) RECOMBINANT PROTEIN - 3286434 3286434
      HUMAN CASPASE-9 (ACTIVE) RECOMBINANT PROTEIN - 3454367 3454367
      HUMAN CASPASE-9 (ACTIVE) RECOMBINANT PROTEIN - 3499840 3499840
      HUMAN CASPASE-9 (ACTIVE) RECOMBINANT PROTEIN - 3541917 3541917

      References

      Reference overviewPub Med ID
      Neuroprotective effect of hyperbaric oxygen therapy in brain injury is mediated by preservation of mitochondrial membrane properties.
      Eilam Palzur,Menashe Zaaroor,Eugene Vlodavsky,Felix Milman,Jean F Soustiel
      Brain research  1221  2008

      Show Abstract
      18561900 18561900
      A conserved coronavirus epitope, critical in virus neutralization, mimicked by internal-image monoclonal anti-idiotypic antibodies.
      C Suñé,C Smerdou,I M Antón,P Abril,J Plana,L Enjuanes
      Journal of virology  65  1991

      Show Abstract Full Text Article
      1719237 1719237

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      Life Science Research > Proteins and Enzymes > Other Enzymes