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APT131 Caspase 3 Colorimetric Activity Assay Kit, DEVD

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      Key Specifications Table

      Species ReactivityKey ApplicationsDetection Methods
      Catalogue NumberAPT131
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionCaspase 3 Colorimetric Activity Assay Kit, DEVD
      OverviewActivation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. Chemicon's Caspase-3 Colorimetric Activity Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD. The assay is based on spectophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate DEVD-pNA. The free pNA can be quantified using a spectrophotometer or a microtiter plate reader at 405 nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-3 activity.
      Alternate Names
      • CPP32
      Materials Required but Not Delivered· Microcentrifuge and 1.5 mL Microcentrifuge tubes

      · 37°C Waterbath or Incubator

      · Spectrophotometer or Microplate Reader

      · 96 well Microtiter Plate
      Product Information
      • 5X Cell Lysis Buffer (Part No. 90065): 5 mL
      • 5X Assay Buffer (Part No. 90066): 10 mL
      • Caspase-3 Substrate (Ac-DEVD-pNA) (Part No. 90079): 250 μL of 3 mg/mL solution
      • Caspase-3 Inhibitor (Ac-DEVD-CHO) (Part No. 90067): 50 μL of 100 μM (0.05 mg/mL) in DMSO
      • pNA Standard (Part No. 90085): 250 μL of 10 mM in DMSO
      Detection methodChromogenic
      HS Code3002 15 90
      Quality LevelMQ100
      ApplicationCaspase-3 Colorimetric Activity Assay Kits provide a simple & convenient means for assaying the activity of caspases that recognize the sequence DEVD.
      Key Applications
      • Activity Assay
      Biological Information
      Species Reactivity
      • Mammals
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.
      Gene Symbol
      • CASP3
      • apopain
      • procaspase3
      • CPP-32
      • Apopain
      • Yama
      • CASP-3
      • CPP32B
      • SCA-1
      • CPP32
      • EC [Contains: Caspase-3 subunit p17
      • Caspase-3 subunit p12].
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P42574 # Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp- -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.
      SIZE: 277 amino acids; 31608 Da
      SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit.
      TISSUE SPECIFICITY: Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
      PTM: Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
      SIMILARITY: SwissProt: P42574 ## Belongs to the peptidase C14 family.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore kit materials at -20°C up to their expiration date.

      Special Precautions:

      · After thawing reagents, use immediately or aliquot and freeze at -20°C for longer storage. Avoid repeated freeze/thaw cycles.

      · The Caspase-3 substrate and pNA standard are especially light sensitive. Maintain these reagents in amber or covered containers.
      Packaging Information
      Material Size25 assays
      Transport Information
      Supplemental Information
      Global Trade Item Number
      Catalog Number GTIN
      APT131 04053252515293


      Caspase 3 Colorimetric Activity Assay Kit, DEVD SDS


      Safety Data Sheet (SDS) 


      Reference overviewSpeciesPub Med ID
      Enoxacin directly inhibits osteoclastogenesis without inducing apoptosis.
      Edgardo J Toro,Jian Zuo,David A Ostrov,Dana Catalfamo,Vivian Bradaschia-Correa,Victor Arana-Chavez,Aliana R Caridad,John K Neubert,Thomas J Wronski,Shannon M Wallet,L Shannon Holliday
      The Journal of biological chemistry  287  2012

      Show Abstract
      22474295 22474295
      Blueberry anthocyanins and pyruvic acid adducts: anticancer properties in breast cancer cell lines.
      Ana Faria,Diogo Pestana,Diana Teixeira,Victor de Freitas,Nuno Mateus,Conceição Calhau
      Phytotherapy research : PTR  24  2010

      Show Abstract
      20564502 20564502
      The antioxidant effect exerted by TGF-1beta-stimulated hyaluronan production reduced NF-kB activation and apoptosis in human fibroblasts exposed to FeSo4 plus ascorbate.
      Giuseppe M Campo, Angela Avenoso, Salvatore Campo, Angela D'Ascola, Paola Traina, Dario Samà, Alberto Calatroni
      Molecular and cellular biochemistry  311  167-77  2008

      Show Abstract
      18224424 18224424
      NF-kB and caspases are involved in the hyaluronan and chondroitin-4-sulphate-exerted antioxidant effect in fibroblast cultures exposed to oxidative stress.
      Giuseppe M Campo, Angela Avenoso, Salvatore Campo, Angela D'Ascola, Paola Traina, Dario Samà, Alberto Calatroni
      Journal of applied toxicology : JAT  28  509-17  2008

      Show Abstract
      17879260 17879260
      Detection of caspase-3, neuron specific enolase, and high-sensitivity C-reactive protein levels in both cerebrospinal fluid and serum of patients after aneurysmal subarachnoid hemorrhage.
      Tibet Kacira, Rahsan Kemerdere, Pinar Atukeren, Hakan Hanimoglu, Galip Zihni Sanus, Mine Kucur, Taner Tanriverdi, Koray Gumustas, Mehmet Yasar Kaynar
      Neurosurgery  60  674-9; discussion 679-80  2007

      Show Abstract
      17415204 17415204
      Evaluation of apoptosis in cerebrospinal fluid of patients with severe head injury.
      M Uzan, H Erman, T Tanriverdi, G Z Sanus, A Kafadar, H Uzun
      Acta neurochirurgica  148  1157-64; discussion  2006

      Show Abstract
      16964558 16964558
      Endoplasmic reticulum stress-induced programmed cell death in soybean cells
      Zuppini, A. et al.
      J. Cell Sci., 117(Pt 12):2591-2598 (2004)  2004

      Green Plants15159454 15159454