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CC126 Caspase 1, recombinant human active

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CC126
25 units  
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      Overview

      Replacement Information

      Key Specifications Table

      Key ApplicationsEntrez Gene NumberSpeciesUni Prot Number
      ACTNM_001223.3 NM_033292.2 NM_033293.2 NM_033294.2 NM_033295.2 Human P29466
      Description
      Catalogue NumberCC126
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionCaspase 1, recombinant human active
      OverviewCaspase-1 (also known as ICE, Interleukin-1beta-Converting Enzyme) is the prototypical member of the caspase family of cysteine proteases. Caspase-1 exists in cells as an inactive 45 kDa pro-enzyme. The pro-enzyme is matured by proteolysis to yield large (20 kD) and small (10 kD) subunits. The active enzyme is a heterotetramer consisting of two large and two small subunits. To date the mechanism of regulation of caspase-1 activation is complex and poorly understood. In THP-1 cells, a large proportion of the caspase-1 is present in the inactive pro-enzyme form.

      The active recombinant human caspase-1 was expressed in E. coli. The expressed caspase-1 spontaneously undergoes autoprocessing to yield the subunits characteristic of the native enzyme. The recombinant caspase-1 preferentially cleaves the substrates consisting of consensus sequence YVAD (e.g., YVAD-AFC and YVAD-pNA). The recombinant enzyme can also be used as a positive control in caspase assays or in determining the specificity of caspase substrates.

      Recommended usage: 0.5-1 unit for fluorometric caspase assays and 2-4 units for colorimetric caspase assays. Optimal working dilutions must be determined by end user.
      References
      Product Information
      PresentationLyophilized. Reconstitute in 25 μL PBS containing 15% glycerol.
      Quality LevelMQ100
      Applications
      Key Applications
      • Activity Assay
      Biological Information
      PurityPartially purified recombinant caspase 3 with a full length HIS-6-tag. Approximately 10-20% caspase 1 protein. The remainder is E.Coli proteins. Approximately 0.8-2 mg total protein/U of activity; E.Coli proteins have no reactivity.
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing of this gene results in five transcript variants encoding distinct isoforms.
      Gene Symbol
      • CASP1
      • IL1BCE
      • P45
      • IL-1BC
      • IL1BC
      • p45
      • IL1B-convertase
      • CASP-1
      • ICE
      • EC 3.4.22.36 [Contains: Caspase-1 subunit p20
      • Caspase-1 subunit p10].
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P29466 # Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.
      SIZE: 404 amino acids; 45159 Da
      SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunit. The p20 subunit can also form a heterodimer with the epsilon isoform which then has an inhibitory effect. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and NALP2 and whose function would be the activation of proinflammatory caspases.
      SUBCELLULAR LOCATION: Cytoplasm.
      TISSUE SPECIFICITY: Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain.
      PTM: The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
      SIMILARITY: SwissProt: P29466 ## Belongs to the peptidase C14 family. & Contains 1 CARD domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain unopened and rehydrated product at -70°C in undiluted, freeze-thaw protected, tightly sealed aliquots. Avoid repeated freeze/thaw cycles. Product stable for 6 months from date of purchased if handled appropriately.
      Packaging Information
      Material Size25 units
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      CC126 04053252281341

      Documentation

      Caspase 1, recombinant human active Certificates of Analysis

      TitleLot Number
      HUMAN CASPASE-1 (ACTIVE) RECOMBINANT PROTEIN 3150924
      HUMAN CASPASE-1 (ACTIVE) RECOMBINANT PROTEIN 3029801
      HUMAN CASPASE-1 (ACTIVE) RECOMBINANT PROTEIN 3091681
      HUMAN CASPASE-1 (ACTIVE) RECOMBINANT PROTEIN 2930545
      HUMAN CASPASE-1 (ACTIVE) RECOMBINANT PROTEIN 3126999
      HUMAN CASPASE-1 (ACTIVE) RECOMBINANT PROTEIN 3011535
      HUMAN CASPASE-1 (ACTIVE) RECOMBINANT PROTEIN - 2464509 2464509
      HUMAN CASPASE-1 (ACTIVE) RECOMBINANT PROTEIN - 2019754 2019754
      HUMAN CASPASE-1 (ACTIVE) RECOMBINANT PROTEIN - 2042755 2042755
      HUMAN CASPASE-1 (ACTIVE) RECOMBINANT PROTEIN - 2048773 2048773

      References

      Reference overviewPub Med ID
      NKp46 defines ovine cells that have characteristics corresponding to NK cells.
      Timothy Connelley,Anne K Storset,Alan Pemberton,Niall Machugh,Jeremy Brown,Hege Lund,Ivan W Morrison
      Veterinary research  42  2001

      Show Abstract Full Text Article
      21345198 21345198

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      Life Science Research > Proteins and Enzymes > Other Enzymes