Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
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Select A Species, Panel Type, Kit or Sample Type
To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
If you have chosen panel analytes and then choose a premix or single plex kit, you will lose that customization.
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96-Well Plate
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Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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Description
Overview
A cell-permeable indandione compound that inhibits the Bmi1-Ring1A E3 complex ubiquitin ligase activity both in cell-free assays (by 100% against self- and Top2α ubiquitination at 25 and 50 µM, respectively) and in HeLa cells (by 100% against Bmi1 ubiquitination at 50 µM). Shown to greatly potentiate Teniposide (VM26) cancer cytotoxicity (by 10-fold in A375 and A549 cultures at 5.5 and 33 µM, respectively) by preventing Top2α degradation following VM26 treatment (100% inhibition at 50 µM in HeLa cells).
Following reconstitution, aliquot and freeze (-207deg;C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas
Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Documentation
Bmi1-Ring1A E3 Ligase Inhibitor, PRT4165 - CAS 31083-55-3 - Calbiochem Certificates of Analysis
Title
Lot Number
203630
References
Reference overview
Alcganati, I., et al. 2009. PLosOne4, e8104.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
06-September-2013 JSW
Synonyms
2-(Pyridin-3-ylmethylene)-1H-indene-1,3(2H)-dione
Description
A cell-permeable indandione compound that inhibits the activity of the E3 ubiquitin ligase complex Bmi1-Ring1A both in cell-free assays (complete inhibition of self- and Top2α ubiquitination with 25 and 50 µM PRT4165, respectively) and in HeLa cells (complete inhibition of Bmi1 ubiquitination by 50 µM PRT4165; 5 h incubation). Modifies the cellular localization of Bmi1 without affecting its expression levels. Shown to greatly potentiate Teniposide (VM26) cancer cytotoxicity (by 10-fold in A375 melanoma and A549 lung cancer cultures with 5.5 and 33 µM PRT4165, respectively) by preventing Top2α degradation following VM26 treatment (100% inhibition with 50 µM PRT4165 after 4 h 100 µM VM26 treatment in HeLa cells).
Form
Yellow powder
Intert gas (Yes/No)
Packaged under inert gas
CAS number
31083-55-3
Chemical formula
C₁₅H₉NO₂
Structure formula
Purity
≥99% by HPLC
Solubility
DMSO (50 mg/ml). Use only fresh DMSO for reconstitution.
Storage
Protect from light
+2°C to +8°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-207deg;C). Stock solutions are stable for up to 3 months at -20°C.
