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196800 Bax Activator, BAM7 - Calbiochem

196800
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Overview

Replacement Information

Key Specifications Table

Empirical Formula
C₂₁H₁₉N₅O₂S • HBr

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196800-10MG
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      Glass bottle 10 mg
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      Description
      Overview

      This product has been discontinued.



      A cell-permeable pyrazolone compound that triggers Bax conformational changes by targeting Bax N-terminal face "BH3 trigger site" formed by the confluence of α-helices 1 &2 in a reversible manner (IC50 = 4.41 µM against 50 nM BIM BH3-derived peptide for Bax binding), leading to dose-dependent Bax oligomerization induction, Bax mitochondria translocation, and eventual Bax-dependent apoptotic cell death (33%, 90% and 94% survival rate in Bak-/-, Bak-/- Bax-/-, and Bax-/- MEF cultures, respectively; 10 µM for 24 h).

      Catalogue Number196800
      Brand Family Calbiochem®
      SynonymsBax Activating Molecule 7, (E)-4-(2-(2-Ethoxyphenyl)hydrazono)-3-methyl-1-(4-phenylthiazol-2-yl)-1H-pyrazol-5(4H)-one, HBr
      References
      ReferencesGavathiotis, E., et al. 2012. Nat. Chem. Biol. 8, 639.
      Product Information
      FormOrange powder
      Hill FormulaC₂₁H₁₉N₅O₂S • HBr
      Chemical formulaC₂₁H₁₉N₅O₂S • HBr
      ReversibleY
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      Biological Information
      Primary TargetBax
      Purity≥99% by HPLC
      Physicochemical Information
      Cell permeableY
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Bax Activator, BAM7 - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overview
      Gavathiotis, E., et al. 2012. Nat. Chem. Biol. 8, 639.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision28-February-2013 JSW
      SynonymsBax Activating Molecule 7, (E)-4-(2-(2-Ethoxyphenyl)hydrazono)-3-methyl-1-(4-phenylthiazol-2-yl)-1H-pyrazol-5(4H)-one, HBr
      DescriptionA cell-permeable pyrazolone compound that triggers Bax conformational changes by targeting Bax N-terminal face "BH3 trigger site" formed by the confluence of α-helices 1 &2 in a reversible manner (IC50 = 4.41 µM against 50 nM BIM BH3-derived FITC-BIM SAHB peptide for Bax binding), leading to dose-dependent Bax oligomerization induction, Bax mitochondria translocation, and eventual Bax-dependent apoptotic cell death (33%, 90% and 94% survival rate in Bak-/-, Bak-/- Bax-/-, and Bax-/- MEF cultures, respectively; 10 µM for 24 h). BAM7 is shown to exhibit selective affinity toward Bax, but not N-terminal BIM BH3 binding site in the proapoptotic Bak, or the antiapoptotic BCL-XL, MCL-1, and Bfl-1/A1. The N-terminal "Bax trigger site" is distinct from the canonical BH3-binding site at the C-terminal face of Bcl-2 family proteins and Bax K21E mutation is shown to abolish BAM7 binding.
      FormOrange powder
      Intert gas (Yes/No) Packaged under inert gas
      Chemical formulaC₂₁H₁₉N₅O₂S • HBr
      Structure formulaStructure formula
      Purity≥99% by HPLC
      SolubilityDMSO (5 mg/ml)
      Storage +2°C to +8°C
      Protect from light
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Toxicity Standard Handling
      ReferencesGavathiotis, E., et al. 2012. Nat. Chem. Biol. 8, 639.