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71545 BacMagic™ DNA Kit - Novagen

71545
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      Description
      OverviewThe BacMagic™-1 DNA Kit provides a positive selection for creating baculovirus recombinants in preparation for generating recombinant protein expression in insect cells. The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification. BacMagic DNA is an AcNPV genome with a portion of the essential open reading frame (ORF) 1629 deleted, preventing nonrecombinant virus from replicating in insect cells. The target coding sequence in a compatible transfer plasmid, such as pIEx/Bac™, pBAC™, or pTriEx™ vectors, is cotransfected with BacMagic DNA into insect cells. Homologous recombination within the cells restores the function of the viral ORF1629. Only recombinant baculovirus can replicate, producing a homogeneous population of recombinants. The BacMagic™-1 DNA Kit contains the BacMagic-1 DNA, Insect GeneJuice® Transfection Reagent, and a positive control plasmid to monitor co-transfection efficiency.
      Not available for sale in Japan
      Catalogue Number71545
      Brand Family Novagen®
      Application Data
      Figure 1. Construction of baculovirus recombinants with three BacMagic systems

      Figure 2: Streamlined baculovirus method
      References
      Product Information
      Aggregation NumberTRANSFECTION CONTROL PLASMID
      0.5 µgBacMagic DNA
      50 µlInsect GeneJuice Transfection Reagent
      2 µgTransfection Control Plasmid
      Quality LevelMQ100
      Applications
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      Ship Code Blue Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
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