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72157 BacMagic™-2 DNA Kit - Novagen

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      OverviewThe BacMagic™-2 DNA Kit provides a positive selection for creating baculoviruses recombinants in preparation for generating recombinant protein expression in insect cells. The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification. Like BacMagic™ DNA, BacMagic-2 DNA is derived from an AcNPV genome, contains an ORF 1629 deletion which prevents nonrecombinant virus from replicating in insect cells, and contains a deletion of the nonessential chitinase (chiA) gene, to improve production of secreted and membrane-targeted proteins. BacMagic-2 DNA have an additional deletion of the v-cath gene, a cathespsin-like cysteine protease, resulting in significantly improved quality and yield for most target proteins. The BacMagic-2 DNA Kit contains the BacMagic-2 DNA, Insect GeneJuice® Transfection Reagent, and a positive control plasmid to monitor co-transfection efficiency.
      Not available for sale in Japan
      Catalogue Number72157
      Brand Family Novagen®
      Application Data
      Figure 1. Construction of baculovirus recombinants with three BacMagic systems

      Figure 2. Streamlined baculovirus method
      Product Information
      0.5 µgBacMagic-2 DNA
      50 µlInsect GeneJuice Transfection Reagent
      2 µgTransfection Control Plasmid
      DeclarationNot available for sale in Japan.
      Quality LevelMQ100
      Biological Information
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      S PhraseS: 24/25

      Avoid contact with skin and eyes.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information


      BacMagic™-2 DNA Kit - Novagen Certificates of Analysis

      TitleLot Number

      User Protocols

      TB459 BacMagic™ DNA Kits