70235 BL21(DE3) Singles™ Competent Cells - Novagen

70235
View Pricing & Availability

Overview

Replacement Information

Pricing & Availability

Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
70235-3
Retrieving availability...
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Glass bottle 11 rxn
      Retrieving price...
      Price could not be retrieved
      Minimum Quantity needs to be mulitiple of
      Upon Order Completion More Information
      You Saved ()
       
      Request Pricing
      70235-4
      Retrieving availability...
      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
      Limited Quantities Available
      Available
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service
          Contact Customer Service

          Glass bottle 22 rxn
          Retrieving price...
          Price could not be retrieved
          Minimum Quantity needs to be mulitiple of
          Upon Order Completion More Information
          You Saved ()
           
          Request Pricing
          Description
          OverviewA common method for transformation of DNA plasmids into E. coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen prepared competent cells. Novagen® Singles™ Competent Cells are designed for ultimate convenience and reliability in plasmid transformation. Chemically competent cells are prepared using an optimized method. Cells are provided in 50 µl volumes, eliminating the need to aliquot, freeze/thaw, or waste partially used vials, saving time and money and ensuring reliable cell performance. To use, simply thaw, add DNA, incubate 5 minutes on ice, heat shock for 30 seconds, place on ice for 2 minutes, and add SOC medium.
          T7 expression strains are lysogens of bacteriophage DE3, as indicated by the (DE3). These hosts carry a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in appropriate T7 expression vectors, using IPTG as an inducer.
          BL21 has been the gold standard for protein expression since it was first introduced in 1990. Deficient in lon and ompT proteases, BL21 and its derivatives are ideal for many applications.
          Genotype: F ompT hsdSB (rB mB) gal dcm (DE3)


          This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.




          This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
          Catalogue Number70235
          Brand Family Novagen®
          References
          Product Information
          11 rxn or 22 rxnBL21(DE3) Singles Competent Cells
          2 or 4 × 2 mlSOC Medium
          10 µlTest Plasmid
          GenotypeF ompT hsdSB (rB mB) gal dcm (DE3)
          Guaranteed efficiencyGuaranteed efficiency: > 2 x 10⁷ cfu/μg
          Quality LevelMQ100
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Multiple Toxicity Values, refer to MSDS
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          BL21(DE3) Singles™ Competent Cells - Novagen SDS

          Title

          Safety Data Sheet (SDS) 

          BL21(DE3) Singles™ Competent Cells - Novagen Certificates of Analysis

          TitleLot Number
          70235

          Citations

          Title
        • Pokidysheva, E., et al. 2006. Cell 124, 485.
        • Bachler, C., et al. 2005. European Molecular Biology Organization Journal 24, 283.
        • Birgander, P.L., et al. 2005. Journal of Biological Chemistry 280, 14997.
        • Alivan Bueren, A.L., et al. 2005. Journal of Biological Chemistry 280, 530.
        • Chen, M-H., et al. 2005. Journal of Biological Chemistry 280, 10599.
        • Francis, K., et al. 2005. Journal of Biological Chemistry 280, 5195.
        • Hanaoka, S., et al. 2005. Protein Science 14, 119.
        • Hoffmeister, M., et al. 2005. Journal of Biological Chemistry 280, 4329.
        • Hosoya-Matsuda, N., et al. 2005. Journal of Biological Chemistry 280, 840.
        • Hoyos, B., et al. 2005. Journal of Biological Chemistry 280, 6872.
        • Jarvinen, A., et al. 2005. Journal of Biological Chemistry 280, 6595.
        • Kani, K., et al. 2005. Journal of Biological Chemistry 280, 8238.
        • Krug, A., et al. 2005. Journal of Biological Chemistry 280, 585.
        • Lo, K.W.-H., et al. 2005. Journal of Biological Chemistry 280, 8172.
        • Murphy, H.N., et al. 2005. Journal of Biological Chemistry 280, 14524.
        • Nakamaru-Ogiso, E., et al. 2005. Journal of Biological Chemistry 280, 301.
        • Sacchetto, R., et al. 2005. Journal of Biological Chemistry 280, 7147.
        • Smith, J.J., et al. 2005. Protein Science 14, 64.
        • Chen, Y-H., et al. 2004. Nature 429, 675.
        • Codina, A., et al. 2004 Journal of Biological Chemistry 279, 53338.
        • Im, Y.J., et al. 2004. Journal of Biological Chemistry 279, 53451.
        • Katona, L.I., et al. 2004. Journal of Bacteriology 186, 6443.
        • Parker, J.S., et al. 2004. European Molecular Biology Organization Journal 23, 4727.
        • Sivendran, S., et al. 2004. Journal of Biological Chemistry 279, 53789.
        • Wower, I.K., 2004. Journal of Biological Chemistry 279, 54202.
        • User Protocols

          Title
          TB055 pET System Manual

          Related Products & Applications

          Related Products

          Catalog Number Description  
          70181 NovaBlue Singles™ Competent Cells - Novagen Show Pricing & Availability
          70236 BL21(DE3)pLysS Singles™ Competent Cells - Novagen Show Pricing & Availability
          71318 NovaBlue T1R Singles™ Competent Cells - Novagen Show Pricing & Availability
          71400 Rosetta™ 2(DE3) Singles™ Competent Cells - Novagen Show Pricing & Availability
          71408 Origami™ 2(DE3) Singles™ Competent Cells - Novagen Show Pricing & Availability

          Categories

          Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Competent Cells for Expression