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07-1387 Anti-oxidized-CaM Kinase II (Met281/282) Antibody

07-1387
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, ICCRbSerumPolyclonal Antibody
      Description
      Catalogue Number07-1387
      Replaces04-1079
      DescriptionAnti-oxidized-CaM Kinase II (Met281/282) Antibody
      Alternate Names
      • CAMKK beta protein
      • CaM-KK beta
      • CaM-kinase kinase beta
      • CaMKK beta
      • Calcium/calmodulin-dependent protein kinase kinase beta
      • calcium/calmodulin-dependent protein kinase beta
      • calcium/calmodulin-dependent protein kinase kinase 2 beta
      • calcium/calmodulin-dependent protein kinase kinase 2, beta
      Background InformationThe Ca2+/Calmodulin-Dependent Kinase family includes CaM Kinases I through IV, Phosphorylase Kinase, and MLCK. The CaM Kinases are inactive in the absence of stimuli; binding to Ca2+/Calmodulin induces conformational changes and activation. CaM Kinases have broad substrate specificity, with many cellular functions.
      References
      Product Information
      FormatSerum
      PresentationUnpurified rabbit serum containing 0.05% NaN3.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-oxidized-CaM Kinase II (Met281/282) Antibody is validated for use in WB, IC for the detection of oxidized-CaM Kinase II (Met281/282).
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesAn independent lab validated this antibody to work in immunocytochemistry in heart sections from mice treated with saline, AngII,or Iso (Erickson, J.R., 2008).
      Biological Information
      ImmunogenSynthetic peptide encompassing and including oxidized Met281/282.
      EpitopeOxidized Met281/282
      HostRabbit
      SpecificityThis antibody recognizes CaM Kinase II when oxidized on Met281/282.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteHuman. Expected to cross-react on Mouse and Rat based on 100% homology with immunogen sequence.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a delta chain. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Distinct isoforms of this chain have
      different expression patterns.
      Gene Symbol
      • CAMKK
      • CAMKKB
      • EC 2.7.11.17
      • KIAA0787
      • MGC15254
      UniProt Number
      UniProt SummaryFUNCTION: Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Isoform 1, isoform 2 and isoform 3 phosphorylate CAMK1 and CAMK4. Isoform 3 phosphorylates CAMK1D. Isoform 4, isoform 5 and isoform 6 lacking part of the calmodulin-binding domain are inactive. Seems to be involved in hippocampal activation of CREB1 By similarity.

      CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.

      ENZYME REGULATION: Activated by Ca2+/calmodulin. Binding of calmodulin may releave intrasteric autoinhibition. Autophosphorylation does not alter activity or regulation by Ca2+/calmodulin. In part, activity is independent on Ca2+/calmodulin By similarity.

      SUBUNIT STRUCTURE: Interacts with calmodulin By similarity.

      SUBCELLULAR LOCATION: CytoplasmBy similarity.

      TISSUE SPECIFICITY: Ubiquitously expressed with higher levels in the brain. Intermediate levels are detected in spleen, prostate, thyroid and leukocytes. The lowest level is in lung.

      DOMAIN: The autoinhibitory domain overlaps with the calmodulin binding region and may be involved in intrasteric autoinhibition.

      The RP domain (arginine/proline-rich) is involved in the recognition of CAMKI and CAMK4 as substrates By similarity.

      PTM: Autophosphorylated By similarity.

      SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

      Contains 1 protein kinase domain.

      SEQUENCE CAUTION: The sequence CAD38990.1 differs from that shown. Reason: Miscellaneous discrepancy. Intron retention.

      Molecular Weight60 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by western blot on untreated and H2O2-treated mouse heart cell lysate.

      Western Blot Analysis: 1:1,000 dilution of this antibody was used to detect oxidized CaM Kinase II in mouse heart cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 years at -20°C from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-oxidized-CaM Kinase II (Met281/282) Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-oxidized-CaM Kinase II (Met281/282) Antibody Certificates of Analysis

      TitleLot Number
      Anti-oxidized-CaM Kinase II (Met281/282) 2470741
      Anti-oxidized-CaM Kinase II (Met281/282) - 2116007 2116007
      Anti-oxidized-CaM Kinase II (Met281/282) - 2360597 2360597
      Anti-oxidized-CaM Kinase II (Met281/282) - 2391002 2391002
      Anti-oxidized-CaM Kinase II (Met281/282) - 2433544 2433544
      Anti-oxidized-CaM Kinase II (Met281/282) - 2004548 2004548
      Anti-oxidized-CaM Kinase II (Met281/282) - 2055309 2055309
      Anti-oxidized-CaM Kinase II (Met281/282) - 2197257 2197257
      Anti-oxidized-CaM Kinase II (Met281/282) - 2246813 2246813
      Anti-oxidized-CaM Kinase II (Met281/282) - 2280466 2280466