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07-585 Anti-dimethyl-Histone H3 (Arg2) Antibody

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Ch, HWB, PIARbSerumPolyclonal Antibody
      Catalogue Number07-585
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-dimethyl-Histone H3 (Arg2) Antibody
      Alternate Names
      • H3R2me2
      • Histone H3 (di methyl R2)
      Product Information
      Presentationrabbit antiserum containing 0.05% sodium azide, before the addition of glycerol to 30%
      Quality LevelMQ100
      ApplicationAnti-dimethyl-Histone H3 (Arg2) Antibody is a Rabbit Polyclonal Antibody for detection of dimethyl-Histone H3 (Arg2) also known as H3R2me2, Histone H3 (di methyl R2) & has been validated in WB, PIA.
      Key Applications
      • Western Blotting
      • Peptide Inhibition Assay
      Biological Information
      Immunogenpeptide containing the sequence AdimRT, corresponding to dimethyl-arginine 2 of human histone H3
      Specificitydimethyl-arginine at residue 2 of histone H3
      Species Reactivity
      • Chicken
      • Human
      Species Reactivity NoteBroad species cross-reactivity expected
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family.
      Gene Symbol
      • H3F3A
      • MGC87783
      • H3.3A
      • MGC87782
      • H3F3
      • H3.3B
      • H3F3B
      • Methylation
      Purification MethodSerum
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 136 amino acids; 15508 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity).
      SIMILARITY: SwissProt: Q16695 ## Belongs to the histone H3 family.
      Molecular WeightMr 17kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on acid-extracted proteins from chicken erythrocyte cells (Catalog # 13-107)
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Global Trade Item Number
      Catalog Number GTIN
      07-585 04053252737992


      Anti-dimethyl-Histone H3 (Arg2) Antibody SDS


      Safety Data Sheet (SDS) 

      Anti-dimethyl-Histone H3 (Arg2) Antibody Certificates of Analysis

      TitleLot Number
      Anti-dimethyl-Histone H3 (Arg2) - 2441927 2441927
      Anti-dimethyl-Histone H3 (Arg2) - 2278513 2278513
      Anti-dimethyl-Histone H3 (Arg2) - 26955 26955
      Anti-dimethyl-Histone H3 (Arg2) - 3288521 3288521
      Anti-dimethyl-Histone H3 (Arg2) - 3474219 3474219
      Anti-dimethyl-Histone H3 (Arg2) - 3679448 3679448
      Anti-dimethyl-Histone H3 (Arg2) - 3852285 3852285
      Anti-dimethyl-Histone H3 (Arg2) - DAM1531013 DAM1531013
      Anti-dimethyl-Histone H3 (Arg2) - DAM1731499 DAM1731499
      Anti-dimethyl-Histone H3 (Arg2) - DAM1776469 DAM1776469


      Reference overviewApplicationSpeciesPub Med ID
      PADI4 acts as a coactivator of Tal1 by counteracting repressive histone arginine methylation.
      Kolodziej, S; Kuvardina, ON; Oellerich, T; Herglotz, J; Backert, I; Kohrs, N; Buscató, El; Wittmann, SK; Salinas-Riester, G; Bonig, H; Karas, M; Serve, H; Proschak, E; Lausen, J
      Nature communications  5  3995  2014

      Show Abstract
      Western Blotting24874575 24874575
      PELP1 oncogenic functions involve alternative splicing via PRMT6.
      Mann, M; Zou, Y; Chen, Y; Brann, D; Vadlamudi, R
      Molecular oncology  8  389-400  2014

      Show Abstract
      24447537 24447537
      The arginine methyltransferase PRMT6 regulates cell proliferation and senescence through transcriptional repression of tumor suppressor genes.
      Stein, C; Riedl, S; Rüthnick, D; Nötzold, RR; Bauer, UM
      Nucleic acids research  40  9522-33  2012

      Show Abstract
      22904088 22904088
      Systematic dissection of roles for chromatin regulators in a yeast stress response.
      Weiner, A; Chen, HV; Liu, CL; Rahat, A; Klien, A; Soares, L; Gudipati, M; Pfeffner, J; Regev, A; Buratowski, S; Pleiss, JA; Friedman, N; Rando, OJ
      PLoS biology  10  e1001369  2012

      Show Abstract
      22912562 22912562
      Cell cycle regulation by the PRMT6 arginine methyltransferase through repression of cyclin-dependent kinase inhibitors.
      Kleinschmidt, MA; de Graaf, P; van Teeffelen, HA; Timmers, HT
      PloS one  7  e41446  2012

      Show Abstract
      22916108 22916108
      Coordinated regulation of active and repressive histone methylations by a dual-specificity histone demethylase ceKDM7A from Caenorhabditis elegans.
      Lin, H; Wang, Y; Wang, Y; Tian, F; Pu, P; Yu, Y; Mao, H; Yang, Y; Wang, P; Hu, L; Lin, Y; Liu, Y; Xu, Y; Chen, CD
      Cell research  20  899-907  2010

      Show Abstract
      20567262 20567262
      The solution structure of the first PHD finger of autoimmune regulator in complex with non-modified histone H3 tail reveals the antagonistic role of H3R2 methylation.
      Francesca Chignola, Massimiliano Gaetani, Ana Rebane, Tõnis Org, Luca Mollica, Chiara Zucchelli, Andrea Spitaleri, Valeria Mannella, Pärt Peterson, Giovanna Musco, Francesca Chignola, Massimiliano Gaetani, Ana Rebane, Tõnis Org, Luca Mollica, Chiara Zucchelli, Andrea Spitaleri, Valeria Mannella, Pärt Peterson, Giovanna Musco, Francesca Chignola, Massimiliano Gaetani, Ana Rebane, Tõnis Org, Luca Mollica, Chiara Zucchelli, Andrea Spitaleri, Valeria Mannella, Pärt Peterson, Giovanna Musco
      Nucleic acids research  37  2951-61  2009

      Show Abstract Full Text Article
      19293276 19293276
      PRMT6-mediated methylation of R2 in histone H3 antagonizes H3 K4 trimethylation.
      Hyllus, D; Stein, C; Schnabel, K; Schiltz, E; Imhof, A; Dou, Y; Hsieh, J; Bauer, UM
      Genes & development  21  3369-80  2007

      Show Abstract Full Text Article
      Human18079182 18079182

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