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07-214 Anti-dimethyl-Histone H3 (Arg17) Antibody

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07-214
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, VrtELISA, ICC, WB, PIARbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-214
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-dimethyl-Histone H3 (Arg17) Antibody
      Alternate Names
      • H3R17me2
      • Histone H3 (di methyl R17)
      • H3 histone family, member T
      • histone 3
      • H3
      • histone cluster 3
      • H3
      Background InformationHistone H3.1t (UniProt: Q16695; also known as H3/t, H3tm, H3/g) is encoded by the HIST3H3 (also known as H3FT) gene (Gene ID: 8290) in human. Histone H3 has two main variants, H3.1 and H3.3, which show different genomic localization patterns in animals. The H3.1 and H3.3 complexes also possess distinct histone chaperones, CAF-1 and HIRA, which play important role in mediating DNA-synthesis-dependent and -independent nucleosome assembly. It has been reported that Histone H3.1 serves as the canonical histone, which is incorporated during DNA replication, whereas H3.3 acts as the replacement histone that can be incorporated outside of S-phase during chromatin-disrupting processes like transcription. Histone H 3.1 is a core component of nucleosome that is present only in mammals and is usually enriched in acetylation of Lysine 15 and demethylation of lysine 10 (HeK9Me2). It is expressed during S phase, then expression decreases significantly as cell division slows down during the process of differentiation. Histone H 3.1 expression is shown to be replication dependent. It s presence at the site of UV-induced DNA damage has also been reported. It has also been shown that H3.1/H4 tetramers do not split and remain intact during replication dependent deposition of H3.1 variant. Dimethylation of H3 on Arginine 17 has been linked to gene activation and activation of this modification is linked to cell fate regulation and increases the pluripotency potential of stem cells. In pancreatic beta cells expression of Protein arginine methyltransferase 4 (PRMT4) and demethylation of Arginine 17 is shown to increase under high glucose conditions. (Ref.: Kim, JK et al. (2015). J. Mol. Endocrinol. 54(3); 315-24).
      References
      Product Information
      FormatAffinity Purified
      HS Code3002 15 90
      Control
      • Calf thymus histone preparation, Acid HeLa cell extract.
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-dimethyl-Histone H3 (Arg17) Antibody is a Rabbit Polyclonal Antibody for detection of dimethyl-Histone H3 (Arg17) also known as H3R17me2, Histone H3 (di methyl R17) & has been validated in ELISA, ICC peptide inhibition & WB.
      Key Applications
      • ELISA
      • Immunocytochemistry
      • Western Blotting
      • Peptide Inhibition Assay
      Application NotesPeptide Inhibition Assay Analysis/Western Blotting: A 1:7,500 dilution from a representative lot detected dimethyl-Histone H3 (Arg17) in Histone H3 with CARM1. The dimethylation was inhibited in the presence of peptide containing Di-methyl Arg17.
      ELISA:
      A previous lot of this antibody was used in ELISA. At dilutions greater than 1:2000 this antibody is specific for peptides containing dimethylated Arg17. No cross-reactivity detected with a peptide containing dimethylated Arg26.

      Immunocytochemistry:
      An independent laboratory showed positive immunostaining for CARM-1 specific methylation of Histone H3 in 3134 cells.
      Biological Information
      ImmunogenBSA-conjugated linear peptide corresponding to 11 amino acids from the N-terminal region of human Histone H3 dimethylated on Arginine 17 (methionine removed).
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody specifically detects human Histone H3 dimethylated on Arginine 17.
      IsotypeIgG
      Species Reactivity
      • Human
      • Vertebrates
      Species Reactivity NoteHuman. Broad cross-reactivity expected based on sequence homology among species.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
      Gene Symbol
      • HIST3H3
      • H3FT
      • MGC126886
      • H3t
      • MGC126888
      • H3T
      • H3/g
      • H3.4
      • H3/t
      Modifications
      • Methylation
      Purification MethodImmunoaffinity Chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 136 amino acids; 15508 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      SUBCELLULAR LOCATION: Nucleus.
      TISSUE SPECIFICITY: Expressed in testicular cells.
      DEVELOPMENTAL STAGE: Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
      PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity).
      SIMILARITY: SwissProt: Q16695 ## Belongs to the histone H3 family.
      Molecular Weight~17 kDa observed; 15.51 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Histone H3 with CARM1.

      Western Blotting Analysis: A 1:7,500 dilution of this antibody detected dimethyl-Histone H3 (Arg17) in Histone H3 treated with Histone-arginine methyltransferase (CARM1).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      07-214 04053252515095

      Documentation

      Anti-dimethyl-Histone H3 (Arg17) Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-dimethyl-Histone H3 (Arg17) Antibody Certificates of Analysis

      TitleLot Number
      Anti-dimethyl-Histone H3 (Arg17)
      Anti-dimethyl-Histone H3 (Arg17) 3079420
      Anti-dimethyl-Histone H3 (Arg17) 2470870
      Anti-dimethyl-Histone H3 (Arg17) - 20451 20451
      Anti-dimethyl-Histone H3 (Arg17) - 23812 23812
      Anti-dimethyl-Histone H3 (Arg17) - 24101 24101
      Anti-dimethyl-Histone H3 (Arg17) - 25045 25045
      Anti-dimethyl-Histone H3 (Arg17) - 3179198 3179198
      Anti-dimethyl-Histone H3 (Arg17) - 3427265 3427265
      Anti-dimethyl-Histone H3 (Arg17) - 3722141 3722141

      References

      Reference overviewApplicationSpeciesPub Med ID
      The HPV E6 oncoprotein targets histone methyltransferases for modulating specific gene transcription.
      Hsu, CH; Peng, KL; Jhang, HC; Lin, CH; Wu, SY; Chiang, CM; Lee, SC; Yu, WC; Juan, LJ
      Oncogene  31  2335-49  2012

      Show Abstract
      Western Blotting21963854 21963854
      Protein arginine methyltransferase 7 regulates cellular response to DNA damage by methylating promoter histones H2A and H4 of the polymerase δ catalytic subunit gene, POLD1.
      Karkhanis, V; Wang, L; Tae, S; Hu, YJ; Imbalzano, AN; Sif, S
      The Journal of biological chemistry  287  29801-14  2012

      Show Abstract
      22761421 22761421
      Ablation of PRMT6 reveals a role as a negative transcriptional regulator of the p53 tumor suppressor.
      Neault, M; Mallette, FA; Vogel, G; Michaud-Levesque, J; Richard, S
      Nucleic acids research  40  9513-21  2012

      Show Abstract
      Western Blotting22904064 22904064
      The expression of myogenic microRNAs indirectly requires protein arginine methyltransferase (Prmt)5 but directly requires Prmt4.
      Mallappa, C; Hu, YJ; Shamulailatpam, P; Tae, S; Sif, S; Imbalzano, AN
      Nucleic acids research  39  1243-55  2011

      Show Abstract
      Western Blotting20947566 20947566
      HuD interacts with survival motor neuron protein and can rescue spinal muscular atrophy-like neuronal defects.
      Hubers, L; Valderrama-Carvajal, H; Laframboise, J; Timbers, J; Sanchez, G; Côté, J
      Human molecular genetics  20  553-79  2011

      Show Abstract
      21088113 21088113
      Carbon monoxide stimulates global protein methylation via its inhibitory action on cystathionine β-synthase.
      Yamamoto T, Takano N, Ishiwata K, Suematsu M
      J Clin Biochem Nutr  48  96-100. Epub 2010 Dec 28.  2011

      Show Abstract Full Text Article
      21297920 21297920
      Effects of a novel arginine methyltransferase inhibitor on T-helper cell cytokine production.
      Bonham, K; Hemmers, S; Lim, YH; Hill, DM; Finn, MG; Mowen, KA
      The FEBS journal  277  2096-108  2010

      Show Abstract Full Text Article
      20345902 20345902
      Ordered transcriptional factor recruitment and epigenetic regulation of tnf-alpha in necrotizing acute pancreatitis.
      Sandoval J, Pereda J, Rodriguez JL, Escobar J, Hidalgo J, Joosten LA, Franco L, Sastre J, López-Rodas G
      Cell Mol Life Sci  67  1687-97. Epub 2010 Feb 4.  2010

      Show Abstract
      20130956 20130956
      Cell-type selective chromatin remodeling defines the active subset of FOXA1-bound enhancers.
      Eeckhoute, J; Lupien, M; Meyer, CA; Verzi, MP; Shivdasani, RA; Liu, XS; Brown, M
      Genome research  19  372-80  2009

      Show Abstract Full Text Article
      19129543 19129543
      Coactivator function defines the active estrogen receptor alpha cistrome.
      Lupien, M; Eeckhoute, J; Meyer, CA; Krum, SA; Rhodes, DR; Liu, XS; Brown, M
      Molecular and cellular biology  29  3413-23  2009

      Show Abstract Full Text Article
      19364822 19364822

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