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09-814 Anti-dimethyl-Arginine Antibody, asymmetric (ASYM25)

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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, H, RWBRbSerumPolyclonal Antibody
      Catalogue Number09-814
      DescriptionAnti-dimethyl-Arginine Antibody, asymmetric (ASYM25)
      Alternate Names
      • dimethyl-arginine, asymmetric
      Background InformationAsymmetrical dimethlarginine (ADMA) is an endogenous, L-arginine metabolite arising from the proteolysis of methylated arginine containing proteins and is a known inhibitor of nitric oxide synthase (NOS). In various studies, ADMA has been observed as having a regulatory role in cell motility and the actin cytoskeleton. Elevated levels of ADMA are associated with sporadic FSGS, coronary artery disease, hypertension, peripheral arterial occlusive disease, obesity, stroke, preeclampsia, and cardiovascular mortality/morbidity in patients with diabetic nephropathy.
      Product Information
      • PRMT1 treated and untreated MEF cell lysates
      PresentationUnpurified rabbit polyclonal containing serum with 0.05% sodium azide.
      Quality LevelMQ100
      ApplicationDetect dimethyl-Arginine using this Anti-dimethyl-Arginine Antibody, asymmetric (ASYM25) validated for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated linear peptide containing asymmetric dimethyl-arginine-glycine repeats.
      SpecificityThis antibody recognizes dimethylated arginines.
      Species Reactivity
      • Mouse
      • Human
      • Rat
      Species Reactivity NoteDemonstrated to react with mouse. Predicted to react with human and rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Gene Symbol
      • ASYM25
      • ADMA
      Purification MethodUnpurified
      Molecular WeightMultiple. This antibody detects proteins containing asymmetrical dimethylated arginines.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in PRMT1 treated and untreated MEF cell lysates.

      Western Blot Analysis: 1:1,000 dilution of this antibody detected dimethyl-Arginine on 10 µg of PRMT1 treated and untreated MEF cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Global Trade Item Number
      Catalog Number GTIN
      09-814 04053252288166


      Anti-dimethyl-Arginine Antibody, asymmetric (ASYM25) SDS


      Safety Data Sheet (SDS) 

      Anti-dimethyl-Arginine Antibody, asymmetric (ASYM25) Certificates of Analysis

      TitleLot Number
      Anti-dimethyl-Arginine, asymmetric (ASYM25) - 2384758 2384758
      Anti-dimethyl-Arginine, asymmetric (ASYM25) - 2000402 2000402
      Anti-dimethyl-Arginine, asymmetric (ASYM25) - 2015995 2015995
      Anti-dimethyl-Arginine, asymmetric (ASYM25) - 2051964 2051964
      Anti-dimethyl-Arginine, asymmetric (ASYM25) - 2202498 2202498
      Anti-dimethyl-Arginine, asymmetric (ASYM25) - 2332577 2332577
      Anti-dimethyl-Arginine, asymmetric (ASYM25) - 3184728 3184728
      Anti-dimethyl-Arginine, asymmetric (ASYM25) - 3385826 3385826
      Anti-dimethyl-Arginine, asymmetric (ASYM25) - 3930313 3930313
      Anti-dimethyl-Arginine, asymmetric (ASYM25) - NG1820260 NG1820260


      Reference overviewApplicationPub Med ID
      NNMT promotes epigenetic remodeling in cancer by creating a metabolic methylation sink.
      Ulanovskaya, OA; Zuhl, AM; Cravatt, BF
      Nature chemical biology  9  300-6  2013

      Show Abstract
      Western Blotting23455543 23455543