OP24 Anti-c-K-Ras (Ab-1) Mouse mAb (234-4.2)

OP24
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H, M, R M Monoclonal Antibody

Pricing & Availability

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OP24-100UG
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      Plastic ampoule 100 μg
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      Description
      OverviewRecognizes c-K-Ras and v-K-Ras in SW480 and Y1 cells and normal skin tissue. Cross-reacts with c-H-Ras and c-N-Ras.
      Catalogue NumberOP24
      Brand Family Calbiochem®
      References
      ReferencesMizuuchi, H. et al. 1992. Cancer Res. 2, 2777.
      Rodenhuis, S. et al. 1992. Cancer Res. (Suppl) 52, 2665s.
      Sirdransky, D., et al. 1992. Science 256, 102.
      Shaw, P., et al. 1991. Oncogene 6, 2121.
      Bos, J.L. 1989. Cancer Res. 49, 4682.
      Furth, M.E., et al. 1987. Oncogene 1, 47.
      Kraus, M.H., et al. 1984. Proc. Natl. Acad. Sci. USA 81, 5384.
      Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112.
      Taparowsky, E., et al. 1983. Cell 34, 581.
      Ellis, R.W., et al. 1981. Nature 292, 506.
      Shih, T.Y., et al. 1980. Nature 287, 686.
      Product Information
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
      Positive controlSW480 or Y1 cells or normal skin tissue
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesImmunoblotting Palejwala, S. and Goldsmith, L.T. 1991. BioTechniques 11, 606.
      Key Applications Paraffin Sections
      Frozen Sections
      Immunoblotting (Western Blotting)
      Immunoprecipitation
      Application NotesFrozen Sections (10 µg/ml)
      Immunoblotting (5 µg/ml with 1% gelatin, see comments and application references)
      Immunoprecipitation (see comments)
      Paraffin Sections (10 µg/ml, saponin or pepsin pre-treatment required)
      Application CommentsThis antibody reacts with c-K-Ras and v-K-Ras, and cross-reacts with c-H-Ras or c-N- Ras by immunoblotting. The level of expression of p21 Ras is variable in different tissues. For this reason we recommend a sample concentration step prior to immunoblotting (see application references). For immunoprecipitation, use 5 µg Cat. No. OP24 per sample with 45 µl protein A agarose. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenrecombinant, rat K-Ras
      ImmunogenRat
      Epitopewithin amino acids 54-189
      Clone234-4.2
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-c-K-Ras (Ab-1) Mouse mAb (234-4.2) Certificates of Analysis

      TitleLot Number
      OP24

      References

      Reference overview
      Mizuuchi, H. et al. 1992. Cancer Res. 2, 2777.
      Rodenhuis, S. et al. 1992. Cancer Res. (Suppl) 52, 2665s.
      Sirdransky, D., et al. 1992. Science 256, 102.
      Shaw, P., et al. 1991. Oncogene 6, 2121.
      Bos, J.L. 1989. Cancer Res. 49, 4682.
      Furth, M.E., et al. 1987. Oncogene 1, 47.
      Kraus, M.H., et al. 1984. Proc. Natl. Acad. Sci. USA 81, 5384.
      Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112.
      Taparowsky, E., et al. 1983. Cell 34, 581.
      Ellis, R.W., et al. 1981. Nature 292, 506.
      Shih, T.Y., et al. 1980. Nature 287, 686.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision03-June-2009 JSW
      ApplicationFrozen Sections (10 µg/ml)
      Immunoblotting (5 µg/ml with 1% gelatin, see comments and application references)
      Immunoprecipitation (see comments)
      Paraffin Sections (10 µg/ml, saponin or pepsin pre-treatment required)
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with Ag8.653 mouse myeloma cells. Recognizes the ~21 kDa c-K-Ras and v-K-Ras proteins. Also recognizes c-H-Ras and c-N-Ras.
      BackgroundThe human ras gene family consists of three identified members, H, K and N-ras, encoding proteins of 188-189 amino acids and 21,000 (p21) molecular weight. Human H- and K-ras are the homologues of v-H- and v-K-ras sequences originally isolated from Harvey and Kirsten strains of rat sarcoma viruses. Normal human cellular ras genes can be activated to oncogenes by mutations occurring in codons 12, 13 and 61; such mutated, activated and transforming ras genes have been identified and isolated from human tumors and cultured tumor cells. Although the expression patterns of Ras proteins in normal human tissues are known, similar information for activated ras oncogene encoded p21s and their relevance to human disease diagnosis and prognosis is still emerging. K-ras mutations have been identified in several cancers to date including lung and colorectal tumors.
      HostMouse
      Immunogen speciesRat
      Immunogenrecombinant, rat K-Ras
      Epitopewithin amino acids 54-189
      Clone234-4.2
      IsotypeIgG2a
      Specieshuman, mouse, rat
      Positive controlSW480 or Y1 cells or normal skin tissue
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsThis antibody reacts with c-K-Ras and v-K-Ras, and cross-reacts with c-H-Ras or c-N- Ras by immunoblotting. The level of expression of p21 Ras is variable in different tissues. For this reason we recommend a sample concentration step prior to immunoblotting (see application references). For immunoprecipitation, use 5 µg Cat. No. OP24 per sample with 45 µl protein A agarose. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesMizuuchi, H. et al. 1992. Cancer Res. 2, 2777.
      Rodenhuis, S. et al. 1992. Cancer Res. (Suppl) 52, 2665s.
      Sirdransky, D., et al. 1992. Science 256, 102.
      Shaw, P., et al. 1991. Oncogene 6, 2121.
      Bos, J.L. 1989. Cancer Res. 49, 4682.
      Furth, M.E., et al. 1987. Oncogene 1, 47.
      Kraus, M.H., et al. 1984. Proc. Natl. Acad. Sci. USA 81, 5384.
      Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112.
      Taparowsky, E., et al. 1983. Cell 34, 581.
      Ellis, R.W., et al. 1981. Nature 292, 506.
      Shih, T.Y., et al. 1980. Nature 287, 686.
      Application referencesImmunoblotting Palejwala, S. and Goldsmith, L.T. 1991. BioTechniques 11, 606.