PC05L Anti-c-Fos (Ab-2) (4-17) Rabbit pAb

PC05L
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H, M, R Rb Polyclonal Antibody

Pricing & Availability

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PC05L-100UG
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      Glass bottle 100 μg
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      Description
      OverviewRecognizes the ~50-62 kDa c-Fos protein in HepG2, NIH3T3, and MCF-7 cells.
      Catalogue NumberPC05L
      Brand Family Calbiochem®
      References
      ReferencesDeTogni, P., et al. 1988. Cell Biol. 8, 2251.
      Rouscher III, F.J., et al. 1988. Science 240, 1010.
      Sassone-Corsi, P., et al. 1988. Cell 54, 553.
      Verma, I.M. and Graham, W.R. 1987. Cancer Res. 49, 29.
      Verma, I.M. and Sassone-Corsi, P. 1987. Cell 51, 513.
      Muller, R. 1986. Biochim. Biophys. Acta 823, 207.
      Verma, I. 1986. Trends Genet. 2, 93.
      Greenberg, M. and Ziff, E. 1984. Nature (London), 311, 433
      Product Information
      FormLyophilized
      FormulationLyophilized from PBS, a volatile buffer, 100 µg BSA.
      Positive controlHep G2 or NIH 3T3 cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Application ReferencesGel Shift
      Guo, R-F., et al. 2002. Am. J. Pathol. 161, 275.
      Key Applications Immunofluorescence
      Immunoprecipitation
      Not Free-floating Sections
      Paraffin Sections
      Frozen Sections
      Gel Shift
      Immunoblotting (Western Blotting)
      Application NotesFrozen Sections (5-10 µg/ml)
      Immunoblotting (1-5 µg/ml)
      Immunofluorescence (1-5 µg/ml)
      Immunoprecipitation (1-2 µg per reaction)
      Paraffin Sections (5-10 µg/ml, heat or pepsin pre-treatment required)
      Gel Shift (see comments and application references)
      Free-floating Sections (not recommended)
      Application CommentsFor gel shift assays resuspend in 100 µl of buffer. The immunogen, c-fos (Peptide-2), Cat. No. PP10, is also available for competition studies (see Murphy, et al. below). Antibodies should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide (SGFNADYEASSSRC) (Cat. No. PP10) corresponding to amino acids 4-17 of human c-Fos
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsResuspend the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-c-Fos (Ab-2) (4-17) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-c-Fos (Ab-2) (4-17) Rabbit pAb Certificates of Analysis

      TitleLot Number
      PC05L

      References

      Reference overview
      DeTogni, P., et al. 1988. Cell Biol. 8, 2251.
      Rouscher III, F.J., et al. 1988. Science 240, 1010.
      Sassone-Corsi, P., et al. 1988. Cell 54, 553.
      Verma, I.M. and Graham, W.R. 1987. Cancer Res. 49, 29.
      Verma, I.M. and Sassone-Corsi, P. 1987. Cell 51, 513.
      Muller, R. 1986. Biochim. Biophys. Acta 823, 207.
      Verma, I. 1986. Trends Genet. 2, 93.
      Greenberg, M. and Ziff, E. 1984. Nature (London), 311, 433

      Citations

      Title
    • Abigail Derbyshire and Mike Ludwig. (2004) TFF3 induced Fos protein expression in the magnocellular oxytocin neurons of the hypothalamus. Peptides 25, 833-838.
    • Ren-Feng Guo, et al. (2002) Activator Protein-1 Actvation in Acute Lung Injury. American Journal of Pathology 161, 275-282.
    • Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision17-September-2015 JSW
      ApplicationFrozen Sections (5-10 µg/ml)
      Immunoblotting (1-5 µg/ml)
      Immunofluorescence (1-5 µg/ml)
      Immunoprecipitation (1-2 µg per reaction)
      Paraffin Sections (5-10 µg/ml, heat or pepsin pre-treatment required)
      Gel Shift (see comments and application references)
      Free-floating Sections (not recommended)
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~50-62 kDa c-Fos protein.
      BackgroundThe proto-oncogene fos has been implicated in cell growth, differentiation and development. fos is induced by a large number of stimuli including mitogens, differentiation-specific agents, pharmacological agents, etc. Induction of the 62,000 dalton fos protein is rapid but transient. The fos protein is associated with a 39,000 dalton protein which has been identified as the protein product of the jun proto-oncogene (c-jun). The fos/jun protein complex binds specifically to a sequence element in DNA referred to as the AP-1 binding site.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide (SGFNADYEASSSRC) (Cat. No. PP10) corresponding to amino acids 4-17 of human c-Fos
      IsotypeIgG
      Specieshuman, mouse, rat
      Positive controlHep G2 or NIH 3T3 cells
      FormLyophilized
      FormulationLyophilized from PBS, a volatile buffer, 100 µg BSA.
      PreservativeNone
      CommentsFor gel shift assays resuspend in 100 µl of buffer. The immunogen, c-fos (Peptide-2), Cat. No. PP10, is also available for competition studies (see Murphy, et al. below). Antibodies should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsResuspend the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing.
      Toxicity Standard Handling
      ReferencesDeTogni, P., et al. 1988. Cell Biol. 8, 2251.
      Rouscher III, F.J., et al. 1988. Science 240, 1010.
      Sassone-Corsi, P., et al. 1988. Cell 54, 553.
      Verma, I.M. and Graham, W.R. 1987. Cancer Res. 49, 29.
      Verma, I.M. and Sassone-Corsi, P. 1987. Cell 51, 513.
      Muller, R. 1986. Biochim. Biophys. Acta 823, 207.
      Verma, I. 1986. Trends Genet. 2, 93.
      Greenberg, M. and Ziff, E. 1984. Nature (London), 311, 433
      Citation
    • Abigail Derbyshire and Mike Ludwig. (2004) TFF3 induced Fos protein expression in the magnocellular oxytocin neurons of the hypothalamus. Peptides 25, 833-838.
    • Ren-Feng Guo, et al. (2002) Activator Protein-1 Actvation in Acute Lung Injury. American Journal of Pathology 161, 275-282.
    • Application referencesGel Shift
      Guo, R-F., et al. 2002. Am. J. Pathol. 161, 275.

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      PC05 Anti-c-Fos (Ab-2) (4-17) Rabbit pAb Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies