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OP16L Anti-c-ErbB2/c-Neu (Ab-4) Mouse mAb (7.16.4)

OP16L
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H, R M Monoclonal Antibody

Pricing & Availability

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OP16L-100UG
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      Glass bottle 100 μg
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      OP16L-500UG
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          Glass bottle 500 μg
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          Description
          OverviewRecognizes the ~185 kDa c-ErbB2/c-Neu protein in overexpressing B104-1-1 cells.
          Catalogue NumberOP16L
          Brand Family Calbiochem®
          SynonymsAnti-HER2, Anti-Neu, Anti-ErbB2, Anti-Erythroblastosis Virus
          References
          ReferencesZhang, H., et al. 1999. Exp. Mol. Pathol. 67, 15.
          Peterson, N.C. and Greene, M.I., 1988. DNA Cell Biol. 17, 1031.
          Kokai, Y., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 5389.
          Van De Vijver, M.J., et al. 1988. New Engl. J. Med. 319, 1239.
          DiFiore, P.P., et al. 1987. Science 237, 178.
          Kokai, Y., et al. 1987. Proc. Natl. Acad. Sci. USA 84, 8498.
          Slamon, D.J., et al. Science 235, 177.
          Varley, J.M., et al. 1987. Oncogene 1, 423.
          Bargmann, C.I., et al. 1986. Nature 319, 226.
          Yamamoto, T., et al. 1986. Nature 319, 230.
          Blick, M., et al. 1984. Blood 64, 1234.
          Schwab, M., et al. 1984. Cold Spring Harbor Laboratory 2, 215.
          Product Information
          FormLyophilized
          FormulationLyophilized from a volatile buffer, 100 µg BSA.
          Negative controlFisher rat embryo cells
          Positive controlB104-1-1 cells
          PreservativeNone
          Quality LevelMQ100
          Applications
          Application ReferencesImmunoprecipitation Kokai, Y., et al. 1989. Cell 58, 287. Kokai, Y., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 5389. Frozen Sections Kokai, Y., et al. 1987. Proc. Natl. Acad. Sci. USA 84, 8498. Original Clone, Growth Inhibition Assays Drebin, J.A., et al. 1988. Oncogene 2, 273. Immunoblotting Montgomery, R.B., et al. 2005. Cancer Res. 65, 650.
          Key Applications Immunocytochemistry
          Immunoprecipitation
          Frozen Sections
          Growth Inhibition Assay
          Immunoblotting (Western Blotting)
          Application NotesGrowth Inhibition Assays (see application references)
          Frozen Sections (5 µg/ml, see application references)
          Immunoblotting (see application references)
          Immunocytochemistry (5 µg/ml)
          Immunoprecipitation (1 µg/ml, see application references)
          Application CommentsStains frozen tissue sections of rat cells overexpressing the c-neu protein. This antibody does not inhibit in vitro protein-tyrosine kinase activity, but it will inhibit in vivo growth of tumors overexpressing the neu oncogene (see application references). Does not cross-react with the EGF receptor. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          ImmunogenB104-1-1 cells overexpressing rat c-ErbB2/c-Neu
          ImmunogenHuman
          Epitopewithin the extracellular domain
          Clone7.16.4
          HostMouse
          IsotypeIgG2a
          Species Reactivity
          • Human
          • Rat
          Antibody TypeMonoclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
          Special InstructionsReconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be reconstituted at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage with 0.1% azide. Avoid freeze/thaw cycles of solutions.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-c-ErbB2/c-Neu (Ab-4) Mouse mAb (7.16.4) Certificates of Analysis

          TitleLot Number
          OP16L

          References

          Reference overview
          Zhang, H., et al. 1999. Exp. Mol. Pathol. 67, 15.
          Peterson, N.C. and Greene, M.I., 1988. DNA Cell Biol. 17, 1031.
          Kokai, Y., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 5389.
          Van De Vijver, M.J., et al. 1988. New Engl. J. Med. 319, 1239.
          DiFiore, P.P., et al. 1987. Science 237, 178.
          Kokai, Y., et al. 1987. Proc. Natl. Acad. Sci. USA 84, 8498.
          Slamon, D.J., et al. Science 235, 177.
          Varley, J.M., et al. 1987. Oncogene 1, 423.
          Bargmann, C.I., et al. 1986. Nature 319, 226.
          Yamamoto, T., et al. 1986. Nature 319, 230.
          Blick, M., et al. 1984. Blood 64, 1234.
          Schwab, M., et al. 1984. Cold Spring Harbor Laboratory 2, 215.

          Citations

          Title
        • R. Bruce Montgomery, et al. (2005) Endogenous Anti-HER2 Antibodies Block HER2 Phosphorylation and Signaling through Extracellular Signal-Regulated Kinase. Cancer Research 65, 650-656.
        • Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision16-November-2007 JSW
          SynonymsAnti-HER2, Anti-Neu, Anti-ErbB2, Anti-Erythroblastosis Virus
          ApplicationGrowth Inhibition Assays (see application references)
          Frozen Sections (5 µg/ml, see application references)
          Immunoblotting (see application references)
          Immunocytochemistry (5 µg/ml)
          Immunoprecipitation (1 µg/ml, see application references)
          DescriptionPurified mouse monoclonal antibody generated by immunizing C3H mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes the ~185 kDa c-Erb2/c-neu protein.
          BackgroundAmplification of the human proto-oncogene c-neu occurs in approximately 30% of breast carcinomas and the amplification maybe a useful predictor of disease prognosis. Gene amplification and the resulting overexpression of proto-oncogene encoded proteins is thought to transform cells by chronically stimulating signal transduction pathways and in model systems overexpression of human c-neu induces cellular transformation. In the rat model system, a point mutation has been located within the transmembrane domain of the c-neu oncogene. neu (from neuroglioblastomas) is also referred to as erbB-2 (from erythroblastosis virus) and HER2 (for Human Epidermal Growth Factor Receptor) and was the second of four members of the EGF receptor family to be described.
          HostMouse
          Immunogen speciesHuman
          ImmunogenB104-1-1 cells overexpressing rat c-ErbB2/c-Neu
          Epitopewithin the extracellular domain
          Clone7.16.4
          IsotypeIgG2a
          Specieshuman, rat
          Positive controlB104-1-1 cells
          Negative controlFisher rat embryo cells
          FormLyophilized
          FormulationLyophilized from a volatile buffer, 100 µg BSA.
          PreservativeNone
          CommentsStains frozen tissue sections of rat cells overexpressing the c-neu protein. This antibody does not inhibit in vitro protein-tyrosine kinase activity, but it will inhibit in vivo growth of tumors overexpressing the neu oncogene (see application references). Does not cross-react with the EGF receptor. Antibody should be titrated for optimal results in individual systems.
          Storage +2°C to +8°C
          Do Not Freeze Ok to freeze
          Special InstructionsReconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be reconstituted at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage with 0.1% azide. Avoid freeze/thaw cycles of solutions.
          Toxicity Standard Handling
          ReferencesZhang, H., et al. 1999. Exp. Mol. Pathol. 67, 15.
          Peterson, N.C. and Greene, M.I., 1988. DNA Cell Biol. 17, 1031.
          Kokai, Y., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 5389.
          Van De Vijver, M.J., et al. 1988. New Engl. J. Med. 319, 1239.
          DiFiore, P.P., et al. 1987. Science 237, 178.
          Kokai, Y., et al. 1987. Proc. Natl. Acad. Sci. USA 84, 8498.
          Slamon, D.J., et al. Science 235, 177.
          Varley, J.M., et al. 1987. Oncogene 1, 423.
          Bargmann, C.I., et al. 1986. Nature 319, 226.
          Yamamoto, T., et al. 1986. Nature 319, 230.
          Blick, M., et al. 1984. Blood 64, 1234.
          Schwab, M., et al. 1984. Cold Spring Harbor Laboratory 2, 215.
          Citation
        • R. Bruce Montgomery, et al. (2005) Endogenous Anti-HER2 Antibodies Block HER2 Phosphorylation and Signaling through Extracellular Signal-Regulated Kinase. Cancer Research 65, 650-656.
        • Application referencesImmunoprecipitation Kokai, Y., et al. 1989. Cell 58, 287. Kokai, Y., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 5389. Frozen Sections Kokai, Y., et al. 1987. Proc. Natl. Acad. Sci. USA 84, 8498. Original Clone, Growth Inhibition Assays Drebin, J.A., et al. 1988. Oncogene 2, 273. Immunoblotting Montgomery, R.B., et al. 2005. Cancer Res. 65, 650.

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          OP16 Anti-c-ErbB2/c-Neu (Ab-4) Mouse mAb (7.16.4) Show Pricing & Availability

          Categories

          Life Science Research > Antibodies and Assays > Primary Antibodies