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OP16 Anti-c-ErbB2/c-Neu (Ab-4) Mouse mAb (7.16.4)

OP16
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H, RMMonoclonal Antibody

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OP16-100UG
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      Description
      OverviewRecognizes the ~185 kDa c-ErbB2/c-Neu protein in overexpressing B104-1-1 cells.
      Catalogue NumberOP16
      Brand Family Calbiochem®
      SynonymsAnti-HER2, Anti-c-Neu, Anti-ErbB2, Anti-Erythroblastosis Virus
      Application Data
      Detection of rat c-neu by immunocytochemistry. Sample: B104-1-1 cells overexpressing c-neu. Primary antibody: Anti-c-ErbB2/c-Neu (Ab-4) Mouse mAb (7.16.4) (Cat. No. OP16) (1.4 µg/ml). Detection: fluorescence.
      References
      ReferencesZhang, H., et al. 1999. Exp. Mol. Pathol. 67, 15.
      Peterson, N,C., and Greene, M.I., 1988. DNA Cell Biol. 17, 1031.
      Kokai, Y., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 5389.
      Van De Vijver, M.J., et al. 1988. New Engl. J. Med. 319, 1239.
      DiFiore, P.P., et al. 1987. Science 237, 178.
      Kokai, Y., et al. 1987. Proc. Natl. Acad. Sci. USA 84, 8498.
      Slamon, D.J., et al. Science 235, 177.
      Varley, J.M., et al. 1987. Oncogene 1, 423.
      Bargmann, C.I., et al. 1986. Nature 319, 226.
      Yamamoto, T., et al. 1986. Nature 319, 230.
      Blick, M., et al. 1984. Blood 64, 1234.
      Schwab, M., et al. 1984. Cold Spring Harbor Laboratory 2, 215.
      Product Information
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
      Negative controlFisher rat embryo cells
      Positive controlB104-1-1 cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ200
      Applications
      Application ReferencesImmunoprecipitation Kokai, Y., et al. 1989. Cell 58, 287. Kokai, Y., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 5389. Frozen Sections Kokai, Y., et al. 1987. Proc. Natl. Acad. Sci. USA 84, 8498. Original Clone, Not for Neutralization Studies Drebin, J.A., et al. 1988. Oncogene 2, 273. Immunoblotting Montgomery, R.B., et al. 2005. Cancer Res. 65, 650.
      Key Applications Immunoprecipitation
      Not Neutralization Studies
      Frozen Sections
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Application NotesFrozen Sections (5 µg/ml, see application references)
      Immunoblotting (see application references)
      Immunocytochemistry (5 µg/ml)
      Immunoprecipitation (1 µg/ml, see application references)
      Neutralization Studies (not recommended, see application references)
      Application CommentsStains frozen tissue sections of rat cells overexpressing c-neu. Does not inhibit in vitro protein-tyrosine kinase activity, but it will inhibit in vivo growth of tumors overexpressing the neu oncogene (see application references). Does not cross-react with the EGF receptor. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      ImmunogenB104-1-1 cells overexpressing rat c-ErbB2/c-Neu
      ImmunogenHuman
      EpitopeWithin the extracellular domain
      Clone7.16.4
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Human
      • Rat
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-c-ErbB2/c-Neu (Ab-4) Mouse mAb (7.16.4) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-c-ErbB2/c-Neu (Ab-4) Mouse mAb (7.16.4) Certificates of Analysis

      TitleLot Number
      OP16

      References

      Reference overview
      Zhang, H., et al. 1999. Exp. Mol. Pathol. 67, 15.
      Peterson, N,C., and Greene, M.I., 1988. DNA Cell Biol. 17, 1031.
      Kokai, Y., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 5389.
      Van De Vijver, M.J., et al. 1988. New Engl. J. Med. 319, 1239.
      DiFiore, P.P., et al. 1987. Science 237, 178.
      Kokai, Y., et al. 1987. Proc. Natl. Acad. Sci. USA 84, 8498.
      Slamon, D.J., et al. Science 235, 177.
      Varley, J.M., et al. 1987. Oncogene 1, 423.
      Bargmann, C.I., et al. 1986. Nature 319, 226.
      Yamamoto, T., et al. 1986. Nature 319, 230.
      Blick, M., et al. 1984. Blood 64, 1234.
      Schwab, M., et al. 1984. Cold Spring Harbor Laboratory 2, 215.

      Citations

      Title
    • R. Bruce Montgomery, et al. (2005) Endogenous Anti-HER2 Antibodies Block HER2 Phosphorylation and Signaling through Extracellular Signal-Regulated Kinase. Cancer Research 65, 650-656.
    • Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision02-October-2007 RFH
      SynonymsAnti-HER2, Anti-c-Neu, Anti-ErbB2, Anti-Erythroblastosis Virus
      ApplicationFrozen Sections (5 µg/ml, see application references)
      Immunoblotting (see application references)
      Immunocytochemistry (5 µg/ml)
      Immunoprecipitation (1 µg/ml, see application references)
      Neutralization Studies (not recommended, see application references)
      Application Data
      Detection of rat c-neu by immunocytochemistry. Sample: B104-1-1 cells overexpressing c-neu. Primary antibody: Anti-c-ErbB2/c-Neu (Ab-4) Mouse mAb (7.16.4) (Cat. No. OP16) (1.4 µg/ml). Detection: fluorescence.
      DescriptionPurified mouse monoclonal antibody generated by immunizing C3H mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes the ~185 kDa c-ErbB2/c-neu protein.
      BackgroundAmplification of the human proto-oncogene c-neu occurs in approximately 30% of breast carcinomas and the amplification maybe a useful predictor of disease prognosis. Gene amplification and the resulting overexpression of proto-oncogene encoded proteins is thought to transform cells by chronically stimulating signal transduction pathways and in model systems overexpression of human c-neu induces cellular transformation. In the rat model system, a point mutation has been located within the transmembrane domain of the c-neu oncogene. neu (from neuroglioblastomas) is also referred to as erbB-2 (from erythroblastosis virus) and HER2 (for Human Epidermal Growth Factor Receptor) and was the second of four members of the EGF receptor family to be described.
      HostMouse
      Immunogen speciesHuman
      ImmunogenB104-1-1 cells overexpressing rat c-ErbB2/c-Neu
      EpitopeWithin the extracellular domain
      Clone7.16.4
      IsotypeIgG2a
      Specieshuman, rat
      Positive controlB104-1-1 cells
      Negative controlFisher rat embryo cells
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsStains frozen tissue sections of rat cells overexpressing c-neu. Does not inhibit in vitro protein-tyrosine kinase activity, but it will inhibit in vivo growth of tumors overexpressing the neu oncogene (see application references). Does not cross-react with the EGF receptor. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesZhang, H., et al. 1999. Exp. Mol. Pathol. 67, 15.
      Peterson, N,C., and Greene, M.I., 1988. DNA Cell Biol. 17, 1031.
      Kokai, Y., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 5389.
      Van De Vijver, M.J., et al. 1988. New Engl. J. Med. 319, 1239.
      DiFiore, P.P., et al. 1987. Science 237, 178.
      Kokai, Y., et al. 1987. Proc. Natl. Acad. Sci. USA 84, 8498.
      Slamon, D.J., et al. Science 235, 177.
      Varley, J.M., et al. 1987. Oncogene 1, 423.
      Bargmann, C.I., et al. 1986. Nature 319, 226.
      Yamamoto, T., et al. 1986. Nature 319, 230.
      Blick, M., et al. 1984. Blood 64, 1234.
      Schwab, M., et al. 1984. Cold Spring Harbor Laboratory 2, 215.
      Citation
    • R. Bruce Montgomery, et al. (2005) Endogenous Anti-HER2 Antibodies Block HER2 Phosphorylation and Signaling through Extracellular Signal-Regulated Kinase. Cancer Research 65, 650-656.
    • Application referencesImmunoprecipitation Kokai, Y., et al. 1989. Cell 58, 287. Kokai, Y., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 5389. Frozen Sections Kokai, Y., et al. 1987. Proc. Natl. Acad. Sci. USA 84, 8498. Original Clone, Not for Neutralization Studies Drebin, J.A., et al. 1988. Oncogene 2, 273. Immunoblotting Montgomery, R.B., et al. 2005. Cancer Res. 65, 650.

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies