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MAB1096 Anti-Tumor Necrosis Factor-α Antibody, clone 195

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MAB1096
200 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICC, IHC, NEUT, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB1096
      Replaces04-1114
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Tumor Necrosis Factor-α Antibody, clone 195
      Alternate Names
      • TNF-alpha
      References
      Product Information
      FormatPurified
      HS Code3002 15 90
      PresentationPurified immunoglobulin. Liquid in 0.01M PBS pH 7.1, 0.15M NaCl containing no preservatives. Sterile filtered.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Tumor Necrosis Factor-α Antibody, clone 195 is validated for use in IC, IH, NEUT, WB for the detection of Tumor Necrosis Factor-α.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Neutralizing
      • Western Blotting
      Application NotesWestern Blot

      Immunocytochemistry

      Immunohistochemistry

      Neutralization: 50 ng of TNF-alpha antibody is required for the neutralization of 1 U TNF-alpha. The TNF-alpha-preparation which is used has a specific activity of 2 x 10(7) U/mg protein [cell lytic assay with L929 cells (mouse transformed fibroblasts) and/or WEHJ 164 cells (mouse fibrosarcoma cells)/actinomycin D].

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenHuman, recombinant tumor necrosis factor-alpha produced in E. coli.
      Clone195
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThe antibody reacts with human, natural and recombinant TNF-alpha. lt does not react with human TNF-beta (lymphotoxin). lt does not react with TNF-alpha from mouse, rat, canine, porcine, rhesus-monkey or baboon.

      SPECIES REACTIVITIES:

      Not mouse, rat, canine, porcine, baboon or rhesus-monkey.
      IsotypeIgG3
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
      Gene Symbol
      • TNF
      • OTTHUMP00000037669
      • TNFA
      • TNFSF2
      • cachectin
      • TNF-a
      • Cachectin
      • DIF
      • TNF-alpha
      Non-Reactive Species
      • Canine
      • Mouse
      • Monkey
      • Pig
      • Rat
      • Baboon
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P01375 # Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
      SIZE: 233 amino acids; 25644 Da
      SUBUNIT: Homotrimer.
      SUBCELLULAR LOCATION: Cell membrane; Single-pass type II membrane protein. & Tumor necrosis factor, soluble form: Secreted.
      PTM: The soluble form derives from the membrane form by proteolytic processing. & The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
      DISEASE: SwissProt: P01375 # Cachexia accompanies a variety of diseases, including cancer and infection, and is characterized by general ill health and malnutrition. & Genetic variations in TNF are associated with susceptibility to psoriatic arthritis [MIM:607507]. Psoriasis is a chronic inflammatory dermatosis that affects approximately 2% of the population. It is characterized by red, scaly skin lesions that are usually found on the scalp, elbows, and knees, and may be associated with severe arthritis. Psoriatic arthritis has been defined as an inflammatory arthritis usually without any rheumatoid factor in serum (seronegative arthritis) associated with psoriasis. & Genetic variations in TNF are associated with susceptibility to hepatitis B virus infection (HBV infection) [MIM:610424]. Approximately one third of all cases of cirrhosis and half of all cases of hepatocellular carcinoma can be attributed to chronic HBV infection. HBV infection may result in subclinical or asymptomatic infection, acute self-limited hepatitis, or fulminant hepatitis requiring liver transplantation.
      SIMILARITY: SwissProt: P01375 ## Belongs to the tumor necrosis factor family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain frozen at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. Freeze only once.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MAB1096 04053252267505

      Documentation

      Anti-Tumor Necrosis Factor-α Antibody, clone 195 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Tumor Necrosis Factor-α Antibody, clone 195 Certificates of Analysis

      TitleLot Number
      Anti-Tumor Necrosis Factor-alpha, clone 195 MONOCLONAL ANTIBODY Q2916206
      MOUSE ANTI-TUMOR NECROSIS FACTOR-#945; MONOCLONAL ANTIBODY - 2121752 2121752
      MOUSE ANTI-TUMOR NECROSIS FACTOR-#945; MONOCLONAL ANTIBODY - 2136612 2136612
      MOUSE ANTI-TUMOR NECROSIS FACTOR-a MONOCLONAL ANTIBODY 2935464
      MOUSE ANTI-TUMOR NECROSIS FACTOR-a MONOCLONAL ANTIBODY 2952659
      MOUSE ANTI-TUMOR NECROSIS FACTOR-a MONOCLONAL ANTIBODY - 2110486 2110486
      MOUSE ANTI-TUMOR NECROSIS FACTOR-a MONOCLONAL ANTIBODY - 2299548 2299548
      MOUSE ANTI-TUMOR NECROSIS FACTOR-a MONOCLONAL ANTIBODY - 2332529 2332529
      MOUSE ANTI-TUMOR NECROSIS FACTOR-α - 3488920 3488920
      MOUSE ANTI-TUMOR NECROSIS FACTOR-α - 3698113 3698113

      References

      Reference overviewPub Med ID
      Acute bronchial obstruction in sheep: histopathology and gland cytokine expression.
      Robert A Cox, Ann S Burke, Gloria Oliveras, Perenlei Enkhbaatar, Lillian D Traber, Joseph B Zwischenberger, Marc G Jeschke, Frank C Schmalstieg, David N Herndon, Daniel L Traber, Hal K Hawkins
      Experimental lung research  31  819-37  2005

      Show Abstract
      16684715 16684715
      Monocyte-mediated drug-dependent cellular cytotoxicity: effects on different WEHI 164 target cell lines.
      Austgulen, R, et al.
      Cancer Immunol. Immunother., 22: 176-80 (1986)  1986

      Show Abstract
      3731202 3731202
      Human lymphotoxin.
      Aggarwal, B B
      Meth. Enzymol., 116: 441-8 (1985)  1985

      3878923 3878923
      Human tumor necrosis factor.
      Aggarwal, B B and Kohr, W J
      Meth. Enzymol., 116: 448-56 (1985)  1985

      4088089 4088089

      Data Sheet

      Title
      MOUSE ANTI-TUMOR NECROSIS FACTOR-α

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      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies