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ST1070 Anti-Tiam1 Rabbit pAb

ST1070
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H, M Rb Polyclonal Antibody

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ST1070-50UG
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      Plastic ampoule 50 μg
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      Description
      OverviewRecognizes the ~200 kDa Tiam1 protein in Jurkat and NIH3T3 cells.
      Catalogue NumberST1070
      Brand Family Calbiochem®
      SynonymsAnti-T-Cell Lymphoma Invasion and Metastasis 1
      Application Data
      Detection of mouse and human Tiam1 by immunoblotting. Samples: whole cell lysate from HIH3T3 cells (50 µg, lane 1) and Jurkat cells (25 µg, lane 2). Primary antibody: Anti-Tiam1 Rabbit pAb (Cat. No. ST1070) (1:1000). Detection: chemiluminescence.
      References
      ReferencesMertens, A.E., et al. 2003. FEBS Lett. 546, 11.
      Qi, H., et al. 2001. Cell Growth Differ. 12, 603.
      Stam, J.C., et al. 1997. J. Biol. Chem. 272, 28447.
      Product Information
      FormLiquid
      FormulationIn Tris-Citrate/phosphate, pH 7.0-8.0.
      Positive controlJurkat or NIH3T3 cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (1:1000)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide corresponding to amino acids from the C-terminal domain of human Tiam1
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Highly Toxic
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Tiam1 Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Tiam1 Rabbit pAb Certificates of Analysis

      TitleLot Number
      ST1070

      References

      Reference overview
      Mertens, A.E., et al. 2003. FEBS Lett. 546, 11.
      Qi, H., et al. 2001. Cell Growth Differ. 12, 603.
      Stam, J.C., et al. 1997. J. Biol. Chem. 272, 28447.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision18-October-2007 RFH
      SynonymsAnti-T-Cell Lymphoma Invasion and Metastasis 1
      ApplicationImmunoblotting (1:1000)
      Application Data
      Detection of mouse and human Tiam1 by immunoblotting. Samples: whole cell lysate from HIH3T3 cells (50 µg, lane 1) and Jurkat cells (25 µg, lane 2). Primary antibody: Anti-Tiam1 Rabbit pAb (Cat. No. ST1070) (1:1000). Detection: chemiluminescence.
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~200 kDa Tiam1 protein.
      BackgroundTiam1 (T-cell lymphoma invasion and metastasis 1) was originally identified by its ability to induce invasion by T-lymphoma cells when amplified. Tiam1 is a specific guanine nucleotide exchange factor (GEF) for the GTPase, Rac. By activating Rac1, Tiam1 regulates many cellular functions including actin cytoskeleton dynamics, cell proliferation, and cell survival. Tiam1 activity is regulated by its translocation to the membrane and the subsequent binding of its PH domain to phosphatidylinositol-3,4,5-triphosphate. Tiam1 is cleaved in a caspase-dependent manner in apoptotic cells, rendering it inactive as a result of an inability to translocate to the plasma membrane.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide corresponding to amino acids from the C-terminal domain of human Tiam1
      IsotypeIgG
      Specieshuman, mouse
      Positive controlJurkat or NIH3T3 cells
      FormLiquid
      FormulationIn Tris-Citrate/phosphate, pH 7.0-8.0.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
      Toxicity Highly Toxic
      ReferencesMertens, A.E., et al. 2003. FEBS Lett. 546, 11.
      Qi, H., et al. 2001. Cell Growth Differ. 12, 603.
      Stam, J.C., et al. 1997. J. Biol. Chem. 272, 28447.