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BA24 Anti-Thrombospondin (Ab-3) Mouse mAb (A6.1)

BA24
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
B, Ca, Eq, H, Porcine, R, Sh M Monoclonal Antibody

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BA24-100UG
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      Description
      OverviewRecognizes the ~450 kDa (non-reducing conditions) and the ~180 kDa (reducing conditions) thrombospondin protein, in endothelial cells, fibroblasts, platelets, monocytes, macrophages, and tonsil tissue. Does not cross-react with fibronectin, fibrinogen, or von Willebrand factor.
      Catalogue NumberBA24
      Brand Family Calbiochem®
      SynonymsAnti-TSP-1, Anti-TSP
      Application Data
      Detection of human thombospondin by immunohistochemistry. Sample: Human placenta tissue fixed in formalin and embedded in paraffin. Primary antibody: Anti-Thrombospondin (Ab-3) Mouse mAb (A6.1) (Cat. No. BA24) (1:50). Detection: fluorescence.

      Detection of human Thrombospondin by immunoblotting. Sample: Human thrombospondin. Primary antibody: Anti-Thrombospondin (Ab-3) Mouse mAb (A6.1) (Cat. No. BA24) (1 µg/ml). Detection: chemiluminescence.
      References
      ReferencesDawson, D.W., et al. 1997. J. Cell Biol. 138, 707.
      Alessio, M., et al. 1996. J. Biol. Chem. 271, 1770.
      Silverstein, R.L., et al. 1989. J. Clin. Invest. 84, 546.
      Clemetson, K.J. and McGregor et al., 1987. In Platelets in Biology and Pathology III, MacIntyre, E.E. and Gordon, J.L., eds. Academic Press, Inc.
      Silverstein, R.L. and Nachman, R.L. 1987. J. Clin. Invest. 79, 867.
      Dixit, V.M., et al. 1986. J. Biol. Chem. 261, 1962.
      Silverstein, R.L., et al. 1986. Arteriosclerosis 6, 245.
      Dixit, V.M., et al. 1985. Biochemistry 24, 4270.
      Leung, L.L.K. 1984. J. Clin. Invest. 74, 1764.
      Jaffe, E.A., et al. 1983. PNAS 80, 998.
      Saglio, S. and Slayter, H. 1982. Blood 59, 162.
      Hagen, I. 1975. Biochem. Biophys. Acta. 392, 242.
      Phillips, D.R. and Agin, P.P. 1977. J. Biol. Chem. 252, 2121.
      Product Information
      FormLiquid
      FormulationIn 10 mM PBS, pH 7.4.
      Positive controlEndothelial cells, fibroblasts, platelets, monocytes, or macrophages in tonsil tissue
      PreservativeNone
      Quality LevelMQ100
      Applications
      Application ReferencesNeutralization Studies Galvin, N.J., et al. 1985. J. Cell Biol. 101, 1434.
      Key Applications Immunofluorescence
      Not Paraffin Sections
      Neutralization Studies
      Flow Cytometry
      Frozen Sections
      Immunoblotting (Western Blotting)
      Application NotesElectron Microscopy (see comments)
      Neutralization Studies (see comments and application references)
      Flow Cytometry (see comments)
      Frozen Sections (1:50-1:100)
      Immunoblotting (1:200-1:400)
      Immunofluorescence (see comments)
      Paraffin Sections (not recommended)
      Application CommentsDoes not cross-react with fibronectin, fibrinogen, or von Willebrand factor. This antibody will inhibit TSP-collagen interactions. Its binding to TSP is unaffected by glycosaminoglycans (e.g., hyaluronic acid, chrondroitin sulfate and heparin). EDTA (i.e., low concentrations of Ca2+) enhances the binding capacity of Clone A6.1 to TSP. This antibody has been reported to work in immunofluorescence, flow cytometry, and electron microscopy. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenreduced and alkylated human thrombospondin
      ImmunogenHuman
      CloneA6.1
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Bovine
      • Canine
      • Horse
      • Human
      • Porcine
      • Rat
      • Sheep
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Thrombospondin (Ab-3) Mouse mAb (A6.1) Certificates of Analysis

      TitleLot Number
      BA24

      References

      Reference overview
      Dawson, D.W., et al. 1997. J. Cell Biol. 138, 707.
      Alessio, M., et al. 1996. J. Biol. Chem. 271, 1770.
      Silverstein, R.L., et al. 1989. J. Clin. Invest. 84, 546.
      Clemetson, K.J. and McGregor et al., 1987. In Platelets in Biology and Pathology III, MacIntyre, E.E. and Gordon, J.L., eds. Academic Press, Inc.
      Silverstein, R.L. and Nachman, R.L. 1987. J. Clin. Invest. 79, 867.
      Dixit, V.M., et al. 1986. J. Biol. Chem. 261, 1962.
      Silverstein, R.L., et al. 1986. Arteriosclerosis 6, 245.
      Dixit, V.M., et al. 1985. Biochemistry 24, 4270.
      Leung, L.L.K. 1984. J. Clin. Invest. 74, 1764.
      Jaffe, E.A., et al. 1983. PNAS 80, 998.
      Saglio, S. and Slayter, H. 1982. Blood 59, 162.
      Hagen, I. 1975. Biochem. Biophys. Acta. 392, 242.
      Phillips, D.R. and Agin, P.P. 1977. J. Biol. Chem. 252, 2121.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision10-February-2009 JSW
      SynonymsAnti-TSP-1, Anti-TSP
      ApplicationElectron Microscopy (see comments)
      Neutralization Studies (see comments and application references)
      Flow Cytometry (see comments)
      Frozen Sections (1:50-1:100)
      Immunoblotting (1:200-1:400)
      Immunofluorescence (see comments)
      Paraffin Sections (not recommended)
      Application Data
      Detection of human thombospondin by immunohistochemistry. Sample: Human placenta tissue fixed in formalin and embedded in paraffin. Primary antibody: Anti-Thrombospondin (Ab-3) Mouse mAb (A6.1) (Cat. No. BA24) (1:50). Detection: fluorescence.

      Detection of human Thrombospondin by immunoblotting. Sample: Human thrombospondin. Primary antibody: Anti-Thrombospondin (Ab-3) Mouse mAb (A6.1) (Cat. No. BA24) (1 µg/ml). Detection: chemiluminescence.
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/Ag14 mouse myeloma cells. Recognizes the ~450 kDa (non-reduced) or the ~180 kDa (reduced) thrombospondin (TSP) protein.
      BackgroundThrombospondin (TSP) is a ~450 kDa glycoprotein synthesized by platelets, as well as by monocytes, macrophages, fibroblasts and endothelial cells. Although TSP is the major α-granule protein of human platelets, the concentration of the protein in circulating plasma is quite low. Upon stimulation of platelets, the TSP is expressed on the cell surface where it plays a role in platelet aggregation and other cell-cell interactions. Studies show that the platelet-monocyte association has been demonstrated to be mediated by the interaction of TSP on the platelet surface with a monocyte TSP receptor. The receptor has been identified immunologically as glycoprotein IV, also known as GPIIIb and CD36, a ~90 kDa membrane glycoprotein expressed on platelets, endothelial cells, erythrocytes, monocytes, and some human tumor cells lines but not on lymphocytes or granulocytes. Recent studies have shown that TSP-1 is a naturally occurring inhibitor of angiogenesis whose actions are mediated by CD36, found on microvascular endothelial cells.
      HostMouse
      Immunogen speciesHuman
      Immunogenreduced and alkylated human thrombospondin
      CloneA6.1
      IsotypeIgG₁
      Speciesbovine, canine, horse, human, porcine, rat, sheep
      Positive controlEndothelial cells, fibroblasts, platelets, monocytes, or macrophages in tonsil tissue
      FormLiquid
      FormulationIn 10 mM PBS, pH 7.4.
      Concentration Label Please refer to vial label for lot-specific concentration
      PreservativeNone
      CommentsDoes not cross-react with fibronectin, fibrinogen, or von Willebrand factor. This antibody will inhibit TSP-collagen interactions. Its binding to TSP is unaffected by glycosaminoglycans (e.g., hyaluronic acid, chrondroitin sulfate and heparin). EDTA (i.e., low concentrations of Ca2+) enhances the binding capacity of Clone A6.1 to TSP. This antibody has been reported to work in immunofluorescence, flow cytometry, and electron microscopy. Antibody should be titrated for optimal results in individual systems.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesDawson, D.W., et al. 1997. J. Cell Biol. 138, 707.
      Alessio, M., et al. 1996. J. Biol. Chem. 271, 1770.
      Silverstein, R.L., et al. 1989. J. Clin. Invest. 84, 546.
      Clemetson, K.J. and McGregor et al., 1987. In Platelets in Biology and Pathology III, MacIntyre, E.E. and Gordon, J.L., eds. Academic Press, Inc.
      Silverstein, R.L. and Nachman, R.L. 1987. J. Clin. Invest. 79, 867.
      Dixit, V.M., et al. 1986. J. Biol. Chem. 261, 1962.
      Silverstein, R.L., et al. 1986. Arteriosclerosis 6, 245.
      Dixit, V.M., et al. 1985. Biochemistry 24, 4270.
      Leung, L.L.K. 1984. J. Clin. Invest. 74, 1764.
      Jaffe, E.A., et al. 1983. PNAS 80, 998.
      Saglio, S. and Slayter, H. 1982. Blood 59, 162.
      Hagen, I. 1975. Biochem. Biophys. Acta. 392, 242.
      Phillips, D.R. and Agin, P.P. 1977. J. Biol. Chem. 252, 2121.
      Application referencesNeutralization Studies Galvin, N.J., et al. 1985. J. Cell Biol. 101, 1434.