CB1052 Anti-TUBB2A Mouse mAb (2B2)

CB1052
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H, M, R M Monoclonal Antibody

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CB1052-100UG
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      100 μg
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      Description
      OverviewRecognizes the ~48-50 kDa TUBB2A protein in PC-12, Raw 264.7, Jurkat, and NIH3T3 cells and human spleen tissue.
      Catalogue NumberCB1052
      Brand Family Calbiochem®
      SynonymsAnti-Tubulin β2A
      Application Data
      Detection of rat TUBB2A by immunoblotting. Sample: Whole cell lysate from PC-12 cells. Primary antibody: Anti-TUBB2A Mouse mAb (2B2) (Cat. No. CB1052) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of mouse TUBB2A by immunoblotting. Sample: Whole cell lysate from Raw 264.7 cells. Primary antibody: Anti-TUBB2A Mouse mAb (2B2) (Cat. No. CB1052) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human TUBB2A by immunoblotting. Sample: Whole cell lysate from Jurkat cells. Primary antibody: Anti-TUBB2A Mouse mAb (2B2) (Cat. No. CB1052) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human TUBB2A by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human spleen tissue. Primary antibody: Anti-TUBB2A Mouse mAb (2B2) (Cat. No. CB1052) (1.5 µg/ml). Detection: colorimetric (DAB).
      References
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Negative control293T cells
      Positive controlPC-12 cells, Raw 264.7 cells, Jurkat cells, NIH3T3 cells, Human spleen tissue
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Paraffin Sections
      Application NotesImmunoblotting (1-5 µg/ml)
      Paraffin Sections (1.5 µg/ml)
      Application CommentsVariables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenFull-length, recombinant, human TUBB2A (aa 1-446), expressed as a GST fusion protein
      Clone2B2
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-TUBB2A Mouse mAb (2B2) SDS

      Title

      Safety Data Sheet (SDS) 

      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision01-November-2010 RFH
      SynonymsAnti-Tubulin β2A
      ApplicationImmunoblotting (1-5 µg/ml)
      Paraffin Sections (1.5 µg/ml)
      Application Data
      Detection of rat TUBB2A by immunoblotting. Sample: Whole cell lysate from PC-12 cells. Primary antibody: Anti-TUBB2A Mouse mAb (2B2) (Cat. No. CB1052) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of mouse TUBB2A by immunoblotting. Sample: Whole cell lysate from Raw 264.7 cells. Primary antibody: Anti-TUBB2A Mouse mAb (2B2) (Cat. No. CB1052) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human TUBB2A by immunoblotting. Sample: Whole cell lysate from Jurkat cells. Primary antibody: Anti-TUBB2A Mouse mAb (2B2) (Cat. No. CB1052) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human TUBB2A by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human spleen tissue. Primary antibody: Anti-TUBB2A Mouse mAb (2B2) (Cat. No. CB1052) (1.5 µg/ml). Detection: colorimetric (DAB).
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~48-50 kDa TUBB2A protein.
      BackgroundTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the β-chain and one at a non-exchangeable site on the α-chain.
      HostMouse
      ImmunogenFull-length, recombinant, human TUBB2A (aa 1-446), expressed as a GST fusion protein
      Clone2B2
      IsotypeIgG2a
      Specieshuman, mouse, rat
      Positive controlPC-12 cells, Raw 264.7 cells, Jurkat cells, NIH3T3 cells, Human spleen tissue
      Negative control293T cells
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsVariables associated with assay conditions will dictate the proper working dilution.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review