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574778 Anti-Synapsin I Rabbit pAb

This Anti-Synapsin I Rabbit pAb is validated for use in ELISA, Frozen Sections, Immunoblotting, Immunocytochemistry, Immunoprecipitation for the detection of Synapsin I.

Anti-Synapsin I Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
574778
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
B, H, M, RRbPolyclonal Antibody

Pricing & Availability

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574778-50UL
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      		 Plastic ampoule 50 ul
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      Description
      OverviewRecognizes the synapsin I protein in rat brain.
      Catalogue Number574778
      Brand Family Calbiochem®
      References
      ReferencesStone, L.M., et al. 1994. J. Neurosci. 14, 301.
      Mandell, J.W., et al. 1992. J. Neurosci. 12, 1736.
      Fletcher, T.L., et al. 1991. J. Neurosci. 11, 1617.
      Moore, R.Y., and Berstein, M. 1989. J. Neurosci. 9, 2151.
      Product Information
      FormLiquid
      FormulationUndiluted serum.
      Positive controlRat brain
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Frozen Sections
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Immunoprecipitation
      Application NotesELISA suitable recommend working dilution (1:2500-1:10,000)
      Frozen Sections (1:5000)
      Immunoblotting (1:200, chemiluminescence)
      Immunocytochemistry suitable recommend working dilution (1:500-1:2500)
      Immunoprecipitation suitable recommend working dilution (5 µl/200 µg total protein, see comments)
      Application CommentsExhibits strong reactivity with the synapsin I doublet by immunoblotting. Immunolabeling is blocked by preadsorption of the antibody with synapsin I. 5 µl will quantitatively immunoprecipitate all synapsin in 200 µg total protein from rat brain. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogenpurified, bovine brain synapsin I
      ImmunogenBovine
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Bovine
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      574778-50UL 04055977189742

      Documentation

      Anti-Synapsin I Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Synapsin I Rabbit pAb Certificates of Analysis

      TitleLot Number
      574778

      References

      Reference overview
      Stone, L.M., et al. 1994. J. Neurosci. 14, 301.
      Mandell, J.W., et al. 1992. J. Neurosci. 12, 1736.
      Fletcher, T.L., et al. 1991. J. Neurosci. 11, 1617.
      Moore, R.Y., and Berstein, M. 1989. J. Neurosci. 9, 2151.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision06-July-2022 JSW
      ApplicationELISA suitable recommend working dilution (1:2500-1:10,000)
      Frozen Sections (1:5000)
      Immunoblotting (1:200, chemiluminescence)
      Immunocytochemistry suitable recommend working dilution (1:500-1:2500)
      Immunoprecipitation suitable recommend working dilution (5 µl/200 µg total protein, see comments)
      DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes the synapsin I protein.
      BackgroundSynapsin I is a neuron specific protein that serves as an excellent marker for synaptic terminals. Can be used to estimate synaptic density and synaptogenesis.
      HostRabbit
      Immunogen speciesBovine
      Immunogenpurified, bovine brain synapsin I
      IsotypeIgG
      Speciesbovine, human, mouse, rat
      Positive controlRat brain
      FormLiquid
      FormulationUndiluted serum.
      PreservativeNone
      CommentsExhibits strong reactivity with the synapsin I doublet by immunoblotting. Immunolabeling is blocked by preadsorption of the antibody with synapsin I. 5 µl will quantitatively immunoprecipitate all synapsin in 200 µg total protein from rat brain. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesStone, L.M., et al. 1994. J. Neurosci. 14, 301.
      Mandell, J.W., et al. 1992. J. Neurosci. 12, 1736.
      Fletcher, T.L., et al. 1991. J. Neurosci. 11, 1617.
      Moore, R.Y., and Berstein, M. 1989. J. Neurosci. 9, 2151.