MABD396 Anti-Sortilin Antibody, clone 4E3.1

MABD396
100 μg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M WB, IH(P) M Purified Monoclonal Antibody
      Description
      Catalogue NumberMABD396
      DescriptionAnti-Sortilin Antibody, clone 4E3.1
      Alternate Names
      • Sortilin
      • 100 kDa NT receptor
      • Glycoprotein 95
      • Gp95
      • Neurotensin receptor 3
      • NT3
      • NTR3
      Background InformationSortilin (UniProt Q99523; also known as 100 kDa NT receptor, Glycoprotein 95, Gp95, Neurotensin receptor 3, NT3, NTR3) is encoded by the SORT1 (also known as LDLCQ6, NT3) gene (Gene ID 6272) in human. Sortlin belongs to the a family of sorting receptors characterized by the Vps10p domain that creates a tunnel cavity with its ten-bladed β-propeller for the binding of soluble ligands. The family members also contain a short cytoplasmic tail that harbors recognition motifs for subcellular sorting adaptor proteins. Sortilin is reproted to bind PCSK9 in the trans-Golgi network with high-affinity in HepG2 cells. Sort1 knockout mice exhibit reduced PCSK9 plasma concentration and altered PCSK9 subcellular localization in hepatocytes. Sortilin is also found to colocalize with APP in neurites and facilitates APP processing toward the production of sAPPα, possibly by optimizing APP as a substrate for α-secretase cleavage. Sortilin is initially produced with a signal peptide (a.a. 1-33) and a propeptide (a.a. 34-77) sequence, the removal of which yields the mature receptor (a.a. 78-831) with a large luminal/extracellular (a.a. 78-555) portion, a transmembrane region (a.a. 756-778), and a short cytoplasmic tail (a.a.779-831). Similar to the other two family members (MPRs and SorLA), sortilin resides both on the membrane of intracellular vesicles and on the plasma membrane. Altered sortilin expressions due to SORT1 genetic variations affect serum levels of low density lipoprotein cholesterol (LDL-C) and contribute to the LDL-C level quantitative trait locus 6 (LDLCQ6) and susceptibility to myocardial infarction.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Sortilin Antibody, clone 4E3.1 is an antibody against Sortilin for use in Western Blotting, Immunohistochemistry (Paraffin).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: An 1:250 dilution of this antibody from a representative lot detected Sortilin in human cerebral cortex, colon, and kidney tissue sections.
      Biological Information
      ImmunogenGST-tagged recombinant human Sortilin C-terminal fragment.
      EpitopeC-terminal region.
      Clone4E3.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityExpected to react with both spliced isoforms reported by UniProt (Q99523).
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SORT1
      • LDLCQ6
      • NT3
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~92 kDa observed. Target band size appears larger than the calculated molecular weights of 83.86 kDa (isoform 1) and 69.17 kDa (isoform 2) due to glycosylation. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in E13-14 embryonic mouse brain tissue lysate.

      Western Blotting Analysis: 1.0 µg/mL of this antibody detected Sortilin in 10 µg of E13-14 embryonic mouse brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies