AB9712 Anti-Sortilin Antibody

AB9712
50 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R WB, IHC Rb Serum Polyclonal Antibody
      Description
      Catalogue NumberAB9712
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Sortilin Antibody
      Alternate Names
      • Neurotensin receptor 3
      Background InformationNeurotensin receptor 3 or sortilin (the intracellular sorting protein 95 kDa) is a member of a family of receptors characterized by a single transmembrane domain, a short intracytoplasmic domain, and a long cysteine-rich luminal extracellular domain. This receptor has been found in the brain, spinal cord, muscle and other peripheral cell types, and in cancer cells. Sortilin exhibits sequence similarities with proteins involved in intracellular transport and trafficking.
      References
      Product Information
      FormatSerum
      PresentationLyophilized. Reconstitute with 50 μL of sterile distilled water. Centrifuge to remove any insoluble material.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Sortilin Antibody is an antibody against Sortilin for use in WB, IH.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesWestern blot: 1:1,000-1:2,000 on extracts from NSC34 cells.
      Immunohistochemistry: 1:500-1:1,000 on rat spinal cord.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenRecombinant protein from the extracellular domain of human sortilin.
      HostRabbit
      SpecificitySortilin (Neurotensin receptor 3). By Western blot the antibody recognizes a strong band at ~90 kDa in extracts of NSC34 cells. By immunohistochemistry the antibody intensely stains motor neurons within the rat spinal cord.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a protein that is a multi-ligand type-1 receptor with similarity to the yeast carboxypeptidase Y sorting receptor Vps10 protein. The encoded protein, a trans-Golgi network (TGN) transmembrane protein, binds a number of unrelated ligands that participate in a wide range of cellular processes; however, it lacks the typical features of a signalling receptor. In the TGN, furin mediates the activation of the mature binding form. The encoded protein consists of a large luminal domain, a single transmembrane segment and short C-terminal cytoplasmic tail. The luminal domain contains a cysteine-rich region similar to two corresponding segments in the yeast Vps10p; the cytoplasmic tail is similar to the corresponding segment of the cation-independent mannose 6-phosphate receptor and the tail also interacts with the VHS domains of GGA (Golgi-associated, gamma-adaptin homologous, ARF-interacting) proteins.
      Gene Symbol
      • SORT1
      • NT3
      • Gp95
      • NTR3
      Purification MethodSerum
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q99523 # Functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface. Required for protein transport from the Golgi apparatus to the lysosomes by a pathway that is independent of the mannose-6-phosphate receptor (M6PR). Also required for protein transport from the Golgi apparatus to the endosomes. Promotes neuronal apoptosis by mediating endocytosis of the proapoptotic precursor forms of BDNF (proBDNF) and NGFB (proNGFB). Also acts as a receptor for neurotensin. May promote mineralization of the extracellular matrix during osteogenic differentiation by scavenging extracellular LPL. Probably required in adipocytes for the formation of specialized storage vesicles containing the glucose transporter SLC2A4/GLUT4 (GLUT4 storage vesicles, or GSVs). These vesicles provide a stable pool of SLC2A4 and confer increased responsiveness to insulin. May also mediate transport from the endoplasmic reticulum to the Golgi.
      SIZE: 831 amino acids; 92068 Da
      SUBUNIT: Interacts with LPL and SLC2A4 (By similarity). Interacts with the cytosolic adapter proteins GGA1 and GGA2. Interacts with numerous ligands including the receptor-associated protein LRPAP1/RAP, GM2A and PSAP. Forms a complex with NGFR which binds specifically to the precursor forms of NGFB (proNGFB) and BDNF (proBDNF).
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. Note=Localized to membranes of the endoplasmic reticulum, endosomes, Golgi stack, lysosomes and nucleus. A small fraction of the protein is also localized to the plasma membrane. May also be found in SLC2A4/GLUT4 storage vesicles (GSVs) in adipocytes. Localization to the plasma membrane in adipocytes may be enhanced by insulin.TISSUE SPECIFICITY: Expressed at high levels in brain, spinal cord, heart, skeletal muscle, thyroid, placenta and testis. Expressed at lower levels in lymphoid organs, kidney, colon and liver.
      DOMAIN: SwissProt: Q99523 The N-terminal propeptide may facilitate precursor transport within the Golgi stack. Intrachain binding of the N- terminal propeptide and the extracellular domain may also inhibit premature ligand binding. & The extracellular domain may be shed following protease cleavage in some cell types.
      PTM: The N-terminal propeptide is cleaved by furin and possibly other homologous proteases. & Contains 8 intrachain disulfide bonds. & N-glycosylated.
      SIMILARITY: Contains 9 BNR repeats.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized material at -20 to -70°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. Glycerol (ACS grade or better) can be added 1:1 for additional stability.
      Packaging Information
      Material Size50 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Sortilin Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Sortilin Antibody Certificates of Analysis

      TitleLot Number
      RABBIT ANTI- SORTILIN POLYCLONAL ANTIBODY - 21158822115882
      RABBIT ANTI- SORTILIN POLYCLONAL ANTIBODY - 21200082120008
      RABBIT ANTI- SORTILIN POLYCLONAL ANTIBODY - 21459072145907
      RABBIT ANTI- SORTILIN POLYCLONAL ANTIBODY - 23846382384638
      RABBIT ANTI- SORTILIN - 34488303448830
      RABBIT ANTI- SORTILIN -26043162604316
      RABBIT ANTI- SORTILIN -26681022668102
      RABBIT ANTI- SORTILIN POLYCLONAL ANTIBODY3045108
      RABBIT ANTI- SORTILIN POLYCLONAL ANTIBODY - 20076772007677
      RABBIT ANTI- SORTILIN POLYCLONAL ANTIBODY - 20139272013927

      References

      Reference overviewPub Med ID
      Distribution of NTS3 receptor/sortilin mRNA and protein in the rat central nervous system
      Sarret, P.
      J. Comp. Neurol., 461:483-505 (2003)  2003

      12746864 12746864

      Data Sheet

      Title
      RABBIT ANTI- SORTILIN POLYCLONAL ANTIBODY

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies